Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136

Specificity

Detects human GFAP in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GFAP Biotinylated Antibody

GFAP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

GFAP in Rat Cortical Stem Cells.

GFAP was detected in immersion fixed differentiated rat cortical stem cells using Sheep Anti-Human GFAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GFAP Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human astrocytes and immersion fixed rat cortical stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human GFAP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFAP

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

P14136

Additional GFAP Products

Product Documents for Human GFAP Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GFAP Biotinylated Antibody

For research use only

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Protocols

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