Human/Rat GFAP Antibody

GFAP in Rat Cortical Stem Cells.

GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human GFAP by Simple Western^TM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Rat GFAP by Simple Western^TM.

Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of GFAP by Immunocytochemistry/ Immunofluorescence

Expression of cell markers in primary human Müller cell isolates. (A). Photomicrographs of primary human Müller cell monolayers isolated from eyes of three male cadaveric donors. Scale bars 100 µm. (B). Fluorescent photomicrographs of human Müller cell monolayers immunolabelled for Müller cell markers: intermediate filaments glial fibrillary acidic protein (GFAP, red), vimentin (VIM, green), or glutamine synthetase (GS, green). Negative controls were labelled with species-matched polyclonal or monoclonal antibodies targeted to irrelevant antigens. Primary antibodies: sheep anti-GFAP IgG, mouse anti-VIM IgG1K, rabbit anti-GS IgG. Secondary antibodies: Alexa Fluor 594 donkey anti-sheep IgG (red), Alexa Fluor 488 goat anti-mouse (green), Alexa Fluor 488 goat anti-rabbit (green). Nuclei counterstained with DAPI (blue). Scale bars 50 µm. Abbreviations: DAPI = 4′,6-diamidino-2-phenylindole, GFAP = glial fibrillary acidic protein, GS = glutamine synthetase, Ig = immunoglobulin, VIM = vimentin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37515098), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of GFAP by Immunocytochemistry/ Immunofluorescence

Expression of cell markers in primary human Müller cell isolates. (A). Photomicrographs of primary human Müller cell monolayers isolated from eyes of three male cadaveric donors. Scale bars 100 µm. (B). Fluorescent photomicrographs of human Müller cell monolayers immunolabelled for Müller cell markers: intermediate filaments glial fibrillary acidic protein (GFAP, red), vimentin (VIM, green), or glutamine synthetase (GS, green). Negative controls were labelled with species-matched polyclonal or monoclonal antibodies targeted to irrelevant antigens. Primary antibodies: sheep anti-GFAP IgG, mouse anti-VIM IgG1K, rabbit anti-GS IgG. Secondary antibodies: Alexa Fluor 594 donkey anti-sheep IgG (red), Alexa Fluor 488 goat anti-mouse (green), Alexa Fluor 488 goat anti-rabbit (green). Nuclei counterstained with DAPI (blue). Scale bars 50 µm. Abbreviations: DAPI = 4′,6-diamidino-2-phenylindole, GFAP = glial fibrillary acidic protein, GS = glutamine synthetase, Ig = immunoglobulin, VIM = vimentin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37515098), licensed under a CC-BY license. Not internally tested by R&D Systems.