|Detection of Human GGT5 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GGT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GGT5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
GGT5 (Gamma GlutamylTranspeptidase 5; also GGL, GGT-rel and gamma -leukotrienease) is a 74-77 kDa member of the gamma -glutamyltransferase family of enzymes. It is suggested that multiple cell types express GGT5, including neurons, lymphocytes, myocardium and bronchial epithelium. It is also expressed on the surface of endothelial cells, and serves to both metabolize GSH to CysGly, and convert LTC4 to LTD4, an action that appears necessary for targeted inflammatory cell migration. Human GGT5 is a 586 amino acid (aa) type II transmembrane (TM) glycoprotein. It undergoes processing to generate a disulfide-linked heterodimer composed of a 56-58 kDa membrane-embedded heavy chain (aa 1-387, with the TM segment lying between aa 9-29) and a 20-21 kDa light chain (aa 388-586). There is one potential splice variant that shows a deletion of aa 112-133. Over aa 30-586, human GGT5 shares 78% aa identity with mouse GGT5.
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