Human GGT5 Antibody

Catalog # Availability Size / Price Qty
Detection of Human GGT5 by Western Blot.
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Product Details
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Human GGT5 Antibody Summary

Species Reactivity
Detects human GGT5 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GGT5
Accession # P36269
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GGT5 antibody by Western Blot. View Larger

Detection of Human GGT5 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GGT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GGT5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GGT5

GGT5 (Gamma GlutamylTranspeptidase 5; also GGL, GGT-rel and gamma -leukotrienease) is a 74-77 kDa member of the gamma -glutamyltransferase family of enzymes. It is suggested that multiple cell types express GGT5, including neurons, lymphocytes, myocardium and bronchial epithelium. It is also expressed on the surface of endothelial cells, and serves to both metabolize GSH to CysGly, and convert LTC4 to LTD4, an action that appears necessary for targeted inflammatory cell migration. Human GGT5 is a 586 amino acid (aa) type II transmembrane (TM) glycoprotein. It undergoes processing to generate a disulfide-linked heterodimer composed of a 56-58 kDa membrane-embedded heavy chain (aa 1-387, with the TM segment lying between aa 9-29) and a 20-21 kDa light chain (aa 388-586). There is one potential splice variant that shows a deletion of aa 112-133. Over aa 30-586, human GGT5 shares 78% aa identity with mouse GGT5.

Long Name
gamma-Glutamyltransferase 5
Entrez Gene IDs
2687 (Human); 23887 (Mouse)
Alternate Names
DKFZp566O011; EC; gamma-glutamyl cleaving enzyme; Gamma-glutamyl transpeptidase-related enzyme; gamma-glutamyl transpeptidase-related protein; gamma-glutamyltransferase 5; Gamma-glutamyltransferase-like activity 1FLJ92733; Gamma-glutamyltranspeptidase 5; GGT 5; GGT5; GGTLA1; GGT-rel

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