Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GGT5 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GGT5
Ser30-Tyr586
Accession # P36269

Specificity

Detects human GGT5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GGT5 Antibody

Detection of Human GGT5 antibody by Western Blot.

Detection of Human GGT5 by Western Blot.

Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GGT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GGT5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GGT5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human kidney (medulla) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GGT5

GGT5 (Gamma GlutamylTranspeptidase 5; also GGL, GGT-rel and gamma -leukotrienease) is a 74-77 kDa member of the gamma -glutamyltransferase family of enzymes. It is suggested that multiple cell types express GGT5, including neurons, lymphocytes, myocardium and bronchial epithelium. It is also expressed on the surface of endothelial cells, and serves to both metabolize GSH to CysGly, and convert LTC4 to LTD4, an action that appears necessary for targeted inflammatory cell migration. Human GGT5 is a 586 amino acid (aa) type II transmembrane (TM) glycoprotein. It undergoes processing to generate a disulfide-linked heterodimer composed of a 56-58 kDa membrane-embedded heavy chain (aa 1-387, with the TM segment lying between aa 9-29) and a 20-21 kDa light chain (aa 388-586). There is one potential splice variant that shows a deletion of aa 112-133. Over aa 30-586, human GGT5 shares 78% aa identity with mouse GGT5.

Long Name

gamma-Glutamyltransferase 5

Alternate Names

GGT-rel, GGTLA1

Entrez Gene IDs

2687 (Human); 23887 (Mouse)

Gene Symbol

GGT5

UniProt

P36269 (Human)

Additional GGT5 Products

Product Documents for Human GGT5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GGT5 Antibody

For research use only

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