Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2352B
View all GLI-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071

Specificity

Detects human GLI-3 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human GLI‑3 Antibody

Detection of GLI-3 in Human SW480 cell line.

GLI‑3 was detected in immersion fixed  SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GLI‑3 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLI-3

GLI3 is a zinc finger protein.  The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling.  GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.

References

Rupert JM., Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton Protein with Multiple Regions of GLI Similarity". Molecular and Cellular Biology. 1990 Oct; 10(10):  5408-15. Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling Pathways in Cancer".  Nature.  2001 May;411(6835):  349-54. Rash BG., Grove EA., "Patterning the Dorsal Telencephalon: a Role for Sonic Hedgehog ?".  The Journal of Neuroscience.  2007 Oct; 27(43):  11595-603.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Entrez Gene IDs

2737 (Human); 14634 (Mouse); 140588 (Rat)

Gene Symbol

GLI3

UniProt

P10071

Additional GLI-3 Products

Product Documents for Human GLI‑3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GLI‑3 Antibody

For research use only

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail