GLI3 is a zinc finger protein. The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling. GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.
Human GLI-3 Antibody (2352B)
R&D Systems | Catalog # MAB3690
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2352B
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Product Specifications
Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Met1-Glu479
Accession # P10071
Specificity
Detects human GLI-3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human GLI-3 Antibody (2352B)
Detection of GLI-3 in Human SW480 cell line.
GLI‑3 was detected in immersion fixed SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GLI-3 Antibody (2352B)
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GLI-3
References
Rupert JM.,
Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton
Protein with Multiple Regions of GLI Similarity". Molecular and
Cellular Biology. 1990 Oct; 10(10): 5408-15.
Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling
Pathways in Cancer". Nature. 2001 May;411(6835): 349-54.
Rash BG., Grove EA., "Patterning the Dorsal Telencephalon:
a Role for Sonic Hedgehog ?". The
Journal of Neuroscience. 2007 Oct; 27(43):
11595-603.
Long Name
GLI-Kruppel family member GLI3
Alternate Names
ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt
Gene Symbol
GLI3
UniProt
Additional GLI-3 Products
Product Documents for Human GLI-3 Antibody (2352B)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GLI-3 Antibody (2352B)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
