Human Glutathione S-Transferase pi 1/GSTP1 Antibody

Catalog # Availability Size / Price Qty
AF6455
AF6455-SP
Human Glutathione S-Transferase pi 1/GSTP1 Antibody in Immunocytochemistry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Glutathione S-Transferase pi 1/GSTP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
Met1-Glu210
Accession # AAC51280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line. View Larger

Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line.
Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Glutathione
S-Transferase pi 1/GSTP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).

References
  1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
  2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
  3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
  4. Hu, X. et al. (1998) Cancer Res. 58:5340.
  5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
  6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
  7. Adler, V. et al. (1999) EMBO J. 18:1321.
Entrez Gene IDs
2950 (Human)
Alternate Names
deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

There are currently no reviews for this product. Be the first to review Human Glutathione S-Transferase pi 1/GSTP1 Antibody and earn rewards!

Have you used Human Glutathione S-Transferase pi 1/GSTP1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review