Human Glutathione S-Transferase pi 1/GSTP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
    Met1-Glu210
    Accession # AAC51280
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line.
Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Glutathione
S-Transferase pi 1/GSTP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione S-Transferase pi 1/GSTP1
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).
  • References:
    1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
    2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
    3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
    4. Hu, X. et al. (1998) Cancer Res. 58:5340.
    5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
    6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
    7. Adler, V. et al. (1999) EMBO J. 18:1321.
  • Entrez Gene IDs:
    2950 (Human)
  • Alternate Names:
    deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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