Human Glycoprotein V/CD42d Antibody

Catalog # Availability Size / Price Qty
AF4249
AF4249-SP
Human Glycoprotein V/CD42d Antibody in Flow Cytometry
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Human Glycoprotein V/CD42d Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glycoprotein V/CD42d in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human GPVI is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
Gln17-Gly523
Accession # P40197
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Glycoprotein V/CD42d (Catalog # 4249-GP)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Human GPV in Human Whole Blood Platelets by Flow Cytometry. View Larger

Detection of Human GPV in Human Whole Blood Platelets by Flow Cytometry.

Human whole blood platelets were stained with Sheep Anti-Human GPV Polyclonal Antibody (Catalog # AF4249) followed by NL637-conjugated anti-sheep antibody (Catalog # NL011) and Human CD41 FITC-conjugated Monoclonal Antibody.Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycoprotein V/CD42d

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

References
  1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
  2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
  3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
  4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
  5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
  6. Wolff, V. et al. (2005) Stroke 36:e17.
  7. Javela, K. et al. (2005) Transfusion 45:1504.
  8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
  9. Kahn, M.L. (1999) Blood 94:4112.
  10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
  11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
  12. Moog, S. et al. (2001) Blood 98:1038.
  13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
  14. Ni, H. et al. (2001) Blood 98:368.
  15. Andrews, R.K. et al. (2001) Blood 98:681.
Entrez Gene IDs
2814 (Human); 14729 (Mouse); 25259 (Rat)
Alternate Names
platelet glycoprotein V; CD42d; glycoprotein V (platelet); Glycoprotein V; GP5; GPV

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