Human gp130 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB228P-100
FAB228P-025
Detection of gp130 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (6)
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Human gp130 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.
Source
Monoclonal Mouse IgG1 Clone # 28126
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of gp130 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of gp130 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human gp130 PE-conjugated Monoclonal Antibody (Catalog # FAB228P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

References
1. Wang, Y. et al. (1992) Genomics 14:666.
2. Muller-Newen, G. (2003) Sci. STKE pe40.
3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Human gp130 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Interleukin 6/gp130 axis promotes neural invasion in pancreatic cancer
    Authors: H Suzuki, S Mitsunaga, M Ikeda, T Aoyama, K Yoshizawa, M Yamaguchi, M Suzuki, M Narita, T Kawasaki, A Ochiai
    Cancer Medicine, 2022-05-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer
    Authors: MH Kubala, V Punj, VR Placencio-, H Fang, GE Fernandez, R Sposto, YA DeClerck
    Cell Rep, 2018-11-20;25(8):2177-2191.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The IL-6 trans-signaling-STAT3 pathway mediates ECM and cellular proliferation in fibroblasts from hypertrophic scar.
    Authors: Ray S, Ju X, Sun H, Finnerty C, Herndon D, Brasier A
    J Invest Dermatol, 2013-01-10;133(5):1212-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2011-12-23;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010-06-28;17(0):4175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
    Authors: Marino M, Scuderi F, Provenzano C, Scheller J, Rose-John S, Bartoccioni E
    J. Neuroimmunol., 2008-04-08;196(1):41-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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