Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.
Monoclonal Mouse IgG1 Clone # 28126
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
Human whole blood monocytes
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04‑0.2 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF‑1 human erythroleukemic cell line (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228). The ND50 is typically 0.04-0.2 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta,
oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I
transmembrane glycoprotein and member of the type II subfamily, type I
cytokine receptor family. Functionally, it is responsible for
transduction of the IL-6 signal across the plasma membrane (1). Rat
gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa
signal sequence, a 596 aa extracellular domain (ECD), a 22 aa
transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential
N-linked glycosylation sites are found within the rat gp130 ECD (1). The
ECD also contains an N terminal immunoglobulin (Ig)-like C2-type
domain, followed by the cytokine receptor homology region (CHR) which is
made up of two fibronectin type III-like domains and a WSXWS motif, and
three additional fibronectin type III-like domains (2). The domains in
the CHR are the structural hallmarks of the hematopoietic cytokine
receptor family (2). Human gp130 shares 73% and 79% aa sequence identity
with mouse and rat gp130, respectively. Gp130 serves as the signal
transducing receptor subunit for the IL-6-type cytokines consisting of
interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M
(OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1
(NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine
(CLC) (2 - 5). These cytokines are involved in a variety of functions
including the modulation of inflammatory and immune responses, heart
development, fertility, and many other activities (2).
Wang, Y. et al. (1992) Genomics 14:666.
Muller-Newen, G. (2003) Sci. STKE pe40.
Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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