Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04‑0.2 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody.
Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF‑1 human erythroleukemic cell line (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228). The ND50 is typically 0.04-0.2 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta,
oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I
transmembrane glycoprotein and member of the type II subfamily, type I
cytokine receptor family. Functionally, it is responsible for
transduction of the IL-6 signal across the plasma membrane (1). Rat
gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa
signal sequence, a 596 aa extracellular domain (ECD), a 22 aa
transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential
N-linked glycosylation sites are found within the rat gp130 ECD (1). The
ECD also contains an N terminal immunoglobulin (Ig)-like C2-type
domain, followed by the cytokine receptor homology region (CHR) which is
made up of two fibronectin type III-like domains and a WSXWS motif, and
three additional fibronectin type III-like domains (2). The domains in
the CHR are the structural hallmarks of the hematopoietic cytokine
receptor family (2). Human gp130 shares 73% and 79% aa sequence identity
with mouse and rat gp130, respectively. Gp130 serves as the signal
transducing receptor subunit for the IL-6-type cytokines consisting of
interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M
(OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1
(NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine
(CLC) (2 - 5). These cytokines are involved in a variety of functions
including the modulation of inflammatory and immune responses, heart
development, fertility, and many other activities (2).
Wang, Y. et al. (1992) Genomics 14:666.
Muller-Newen, G. (2003) Sci. STKE pe40.
Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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