Human gp130 Antibody

Catalog # Availability Size / Price Qty
MAB228-SP
MAB228-500
MAB228-100
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody.
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Product Details
Citations (10)
FAQs
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Human gp130 Antibody Summary

Species Reactivity
Human
Specificity
Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.
Source
Monoclonal Mouse IgG1 Clone # 28126
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04‑0.2 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. View Larger

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228). The ND50 is typically 0.04-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

References
  1. Wang, Y. et al. (1992) Genomics 14:666.
  2. Muller-Newen, G. (2003) Sci. STKE pe40.
  3.  Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
  4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
  5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Human gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Autocrine STAT3 activation in HPV positive cervical cancer through a virus-driven Rac1—NF kappa B—IL-6 signalling axis
    Authors: Ethan L. Morgan, Andrew Macdonald
    PLOS Pathogens
  2. Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130
    Authors: AN Wilkinson, KH Gartlan, G Kelly, LD Samson, SD Olver, J Avery, N Zomerdijk, SK Tey, JS Lee, S Vuckovic, GR Hill
    J. Immunol., 2018-04-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
    Authors: Ambarus C, Krausz S, van Eijk M, Hamann J, Radstake T, Reedquist K, Tak P, Baeten D
    J Immunol Methods, 2011-10-29;375(1):196-206.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009-01-01;69(1):329-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002-10-15;280(1):24-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  6. Novel IL-6-secreting gamma δT cells increased in patients with atherosclerotic cerebral infarction
    Authors: YOUDI HE, NING MA, CHEN XING, XIAOQIAN WANG, HE XIAO, MINGKE ZHENG et al.
    Molecular Medicine Reports
  7. Structural basis of activation and antagonism of receptor signaling mediated by interleukin-27
    Authors: Katarzyna Składanowska, Yehudi Bloch, Jamie Strand, Kerry F. White, Jing Hua, Daniel Aldridge et al.
    Cell Reports
  8. IL-27 induces IFN/STAT1-dependent genes and enhances function of TIGIT+ HIVGag-specific T cells
    Authors: Jie Cheng, Timothy G. Myers, Callie Levinger, Princy Kumar, Jai Kumar, Bruktawit A. Goshu et al.
    iScience
  9. Chlorin e6 Conjugated Interleukin-6 Receptor Aptamers Selectively Kill Target Cells Upon Irradiation
    Authors: Sven Kruspe, Cindy Meyer, Ulrich Hahn
    Molecular Therapy - Nucleic Acids
  10. Interleukin-6 receptor specific RNA aptamers for cargo delivery into target cells
    Authors: Cindy Meyer, Katja Eydeler, Eileen Magbanua, Tijana Zivkovic, Nicolas Piganeau, Inken Lorenzen et al.
    RNA Biology

FAQs

  1. Has the human gp130 monoclonal antibody (Clone 28126; Catalog # MAB228) been tested in Western blot?

    • Our scientists have found that this antibody does not detect linearized, denatured protein.

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