Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP was stained with either (A) Mouse Anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB6300) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.
G Protein-coupled Receptor, Family D, Group 5, Member D
Entrez Gene IDs:
55507 (Human); 93746 (Mouse); 500349 (Rat)
G protein-coupled receptor, family C, group 5, member D; GPRC5D; G-protein coupled receptor family C group 5 member D; MGC129713; MGC129714; orphan G-protein coupled receptor
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