Human Granulysin DuoSet ELISA

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DY3138
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Human Granulysin ELISA Standard Curve
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Product Details
Citations (6)
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Human Granulysin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Granulysin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Granulysin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granulysin

Granulysin, formerly known as NKG5 or Lymphokine LAG-2, is a membrane-lytic protein that is expressed in granules of natural killer and activated cytotoxic T cells. It exhibits cytolytic activity against intracellular or extracellular microbes and also tumors, either alone or in synergy with Perforin. IL-15 is necessary and sufficient for Granulysin release from CD8+ T cells. Granulysin promotes chemotaxis and increases production of chemokines by monocytic cells as well as target cell lysis. It binds and disrupts microbial membranes through charge interactions.

Entrez Gene IDs:
10578 (Human)
Alternate Names:
D2S69Elymphocyte-activation gene 2; GNLY; Granulysin; LAG2; LAG-2; Lymphokine LAG-2; Lymphokine; NKG5; NKG5519; Protein NKG5; TLA519; TLA519T-cell activation protein 519; T-lymphocyte activation gene 519

Citations for Human Granulysin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Cytotoxic CD8+ T Cells Are Involved in the Thrombo-Inflammatory Response during First-Diagnosed Atrial Fibrillation
    Authors: J Friebel, M Witkowski, M Wegner, L Blöbaum, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, V Moos, HP Schutheiss, U Landmesser, U Rauch
    Cells, 2022-12-29;12(1):.
    Species: Human
    Sample Types: Plasma
  2. Functional and structural characteristics of HLA-B*13:01-mediated specific T cells reaction in dapsone-induced drug hypersensitivity
    Authors: H Jiang, CW Wang, Z Wang, Y Dai, Y Zhu, YS Lee, Y Cao, WH Chung, S Ouyang, H Wang
    Journal of Biomedical Science, 2022-08-13;29(1):58.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. CD127+ CD94+ innate lymphoid cells expressing granulysin and perforin are expanded in patients with Crohn's disease
    Authors: L Krabbendam, BA Heesters, CMA Kradolfer, NJE Haverkate, MAJ Becker, CJ Buskens, WA Bemelman, JH Bernink, H Spits
    Nature Communications, 2021-10-06;12(1):5841.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. &gamma&delta T cells suppress Plasmodium falciparum blood-stage infection by direct killing and phagocytosis
    Authors: C Junqueira, RB Polidoro, G Castro, S Absalon, Z Liang, S Sen Santar, Â Crespo, DB Pereira, RT Gazzinelli, JD Dvorin, J Lieberman
    Nature Immunology, 2021-01-11;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax-infected reticulocytes
    Authors: C Junqueira, CRR Barbosa, PAC Costa, A Teixeira-C, G Castro, S Sen Santar, RP Barbosa, F Dotiwala, DB Pereira, LR Antonelli, J Lieberman, RT Gazzinelli
    Nat. Med., 2018-07-23;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Heightened circulating levels of antimicrobial peptides in tuberculosis-Diabetes co-morbidity and reversal upon treatment
    Authors: NP Kumar, K Moideen, V Viswanatha, S Sivakumar, PA Menon, H Kornfeld, S Babu
    PLoS ONE, 2017-09-14;12(9):e0184753.
    Species: Human
    Sample Types: Plasma

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