Human Granulysin DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human Granulysin ELISA Standard Curve
Preparation and Storage
Granulysin, formerly known as NKG5 or Lymphokine LAG-2, is a membrane-lytic protein that is expressed in granules of natural killer and activated cytotoxic T cells. It exhibits cytolytic activity against intracellular or extracellular microbes and also tumors, either alone or in synergy with Perforin. IL-15 is necessary and sufficient for Granulysin release from CD8+ T cells. Granulysin promotes chemotaxis and increases production of chemokines by monocytic cells as well as target cell lysis. It binds and disrupts microbial membranes through charge interactions.
Citations for Human Granulysin DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax-infected reticulocytes
Authors: C Junqueira, CRR Barbosa, PAC Costa, A Teixeira-C, G Castro, S Sen Santar, RP Barbosa, F Dotiwala, DB Pereira, LR Antonelli, J Lieberman, RT Gazzinelli
Nat. Med., 2018;0(0):.
Sample Types: Plasma
Heightened circulating levels of antimicrobial peptides in tuberculosis-Diabetes co-morbidity and reversal upon treatment
Authors: NP Kumar, K Moideen, V Viswanatha, S Sivakumar, PA Menon, H Kornfeld, S Babu
PLoS ONE, 2017;12(9):e0184753.
Sample Types: Plasma
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