< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Human Haptoglobin Immunoassay is a 4.5 hour solid phase ELISA
designed to measure haptoglobin in serum-free cell culture supernates, serum, plasma, saliva, and urine
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components.
The recovery of human haptoglobin spiked to levels throughout the range
of the assay was evaluated.
Average % Recovery
Serum-free Cell Culture Media (n=4)
To assess the linearity of the assay, samples containing and/or spiked
with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within
the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.
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