Human Haptoglobin Quantikine ELISA Kit

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Human Haptoglobin ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human Haptoglobin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (20 uL)
Sensitivity
0.529 ng/mL
Assay Range
3.1 - 200 ng/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural human haptoglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Haptoglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure haptoglobin in serum-free cell culture supernates, serum, plasma, saliva, and urine

Precision

Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 18.7 58.3 116 18.8 57.8 113
Standard Deviation 0.69 1.42 3.66 1.4 3.47 6.3
CV% 3.7 2.4 3.2 7.4 6 5.6

Recovery

The recovery of human haptoglobin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Serum-free Cell Culture Media (n=4) 101 94-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Haptoglobin ELISA Linearity

Scientific Data

Human Haptoglobin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Haptoglobin

Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.

Entrez Gene IDs:
3240 (Human); 15439 (Mouse); 24464 (Rat)
Alternate Names:
binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 200 µL Assay Diluent
  4.   Add 200 µL of Assay Diluent to each well.

  5. 20 µL Standard, Control, or Sample
  6.   Add 20 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Haptoglobin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The Role of Fractalkine in the Regulation of Endometrial Iron Metabolism in Iron Deficiency
    Authors: Pandur, E;Pap, R;Jánosa, G;Horváth, A;Sipos, K;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Biomarkers of inflammation and epithelial barrier function in multiple sclerosis
    Authors: A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar
    Mult Scler Relat Disord, 2020-09-19;46(0):102520.
    Species: Human
    Sample Types: Serum
  3. Long-lasting severe immune dysfunction in Ebola virus disease survivors
    Authors: A Wiedemann, E Foucat, H Hocini, C Lefebvre, BP Hejblum, M Durand, M Krüger, AK Keita, A Ayouba, S Mély, JC Fernandez, A Touré, S Fourati, C Lévy-March, H Raoul, E Delaporte, L Koivogui, R Thiébaut, C Lacabaratz, Y Lévy
    Nat Commun, 2020-07-24;11(1):3730.
    Species: Human
    Sample Types: Serum
  4. Lipid metabolism impairment in patients with sepsis secondary to hospital acquired pneumonia, a proteomic analysis
    Authors: NK Sharma, BL Ferreira, AK Tashima, MKC Brunialti, RJS Torquato, A Bafi, M Assuncao, LCP Azevedo, R Salomao
    Clin Proteomics, 2019-07-16;16(0):29.
    Species: Human
    Sample Types: Plasma
  5. Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
    Authors: S Wang, J Wang, R Zhang, A Zhao, X Zheng, D Yan, F Jiang, W Jia, C Hu, W Jia
    Cardiovasc Diabetol, 2019-01-11;18(1):8.
    Species: Human
    Sample Types: Serum
  6. Genetic markers for urine haptoglobin is associated with decline in renal function in type 2 diabetes in East Asians
    Authors: RL Gurung, R Dorajoo, S Liu, Y M, JJ Liu, L Wang, L Guo, X Yu, JJ Liu, SC Lim
    Sci Rep, 2018-03-23;8(1):5109.
    Species: Human
    Sample Types: Urine
  7. Mendelian randomization analysis to assess a causal effect of haptoglobin on macroangiopathy in Chinese type 2 diabetes patients
    Authors: S Wang, J Wang, R Zhang, T Wang, D Yan, Z He, F Jiang, C Hu, W Jia
    Cardiovasc Diabetol, 2018-01-16;17(1):14.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human Haptoglobin Quantikine ELISA Kit

Average Rating: 4.5 (Based on 2 Reviews)

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Human Haptoglobin Quantikine ELISA Kit
By Anonymous on 11/16/2022
Sample Tested: Plasma,Urine

Plasma diluted 20,000-fold.
Urine diluted 10-fold with good linearity up to 40-fold as long as values remain in range.


Human Haptoglobin Quantikine ELISA Kit
By Anonymous on 06/29/2018
Sample Tested: EDTA Plasma

HIV-positive samples (ART-naive and ART-stablized)