Human Helios Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Helios in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Helios
    Ile6-Gln97
    Accession # Q9UKS7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Immunoprecipitated material from Jurkat human acute T cell leukemia cell line lysates and MOLT‑4 human acute lymphoblastic leukemia cell line lysates
  • Immunoprecipitation
    4 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation

Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat lysates, MOLT‑4 lysates, or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Immunoprecipitation

Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat or MOLT‑4 lysates were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Helios
Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (amino acids 112-219) and two C-terminal zinc finger domains (amino acids 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within amino acids 6-97, human and mouse Helios share 96% aa sequence identity.
  • Long Name:
    DNA-binding Protein Helios
  • Entrez Gene IDs:
    22807 (Human); 22779 (Mouse)
  • Alternate Names:
    Helios; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; subfamily 1A, 2 (Helios); zinc finger DNA binding protein Helios; zinc finger protein Helios; ZNF1A2; ZNFN1A2
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