Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Helios Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Helios
Ile6-Gln97
Accession # Q9UKS7

Specificity

Detects human Helios in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Helios Antibody

Immunoprecipitation of Human Helios.

Immunoprecipitation of Human Helios.

Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat lysates, MOLT‑4 lysates, or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Immunoprecipitation of Human Helios.

Immunoprecipitation of Human Helios.

Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat or MOLT-4 lysates were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Applications for Human Helios Antibody

Application
Recommended Usage

Immunoprecipitation

4 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates

Western Blot

0.1 µg/mL
Sample: Immunoprecipitated material from Jurkat human acute T cell leukemia cell line lysates and MOLT‑4 human acute lymphoblastic leukemia cell line lysates

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Helios

Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (amino acids 112-219) and two C-terminal zinc finger domains (amino acids 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within amino acids 6-97, human and mouse Helios share 96% aa sequence identity.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Entrez Gene IDs

22807 (Human); 22779 (Mouse)

Gene Symbol

IKZF2

UniProt

Q9UKS7 (Human)

Additional Helios Products

Product Documents for Human Helios Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Helios Antibody

For research use only

Related Research Areas

Regulatory T Cells (Tregs)

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