Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 736423
View all Helios Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Helios
Thr3-Gln97
Accession # Q9UKS7

Specificity

Detects human Helios in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Helios Antibody

Detection of Human Helios antibody by Western Blot.

Detection of Human Helios by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Helios at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Helios antibody in Human Thymus by Immunohistochemistry (IHC-P).

Helios in Human Thymus.

Helios was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cells around Hassall's corpuscles. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Helios Antibody

Application
Recommended Usage

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB73091 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Helios

Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (aa 112-219) and two C-terminal zinc finger domains (aa 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within aa 1-97, human and mouse Helios share 96% aa sequence identity.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Entrez Gene IDs

22807 (Human); 22779 (Mouse)

Gene Symbol

IKZF2

UniProt

Q9UKS7 (Human)

Additional Helios Products

Product Documents for Human Helios Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Helios Antibody

For research use only

Related Research Areas

Regulatory T Cells (Tregs)

Citations for Human Helios Antibody

Customer Reviews for Human Helios Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

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  • Human Helios Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 03/07/2022
    Human Helios Antibody MAB73091
  • Human Helios Antibody
    Name: Masahiro Hirata
    Application: Immunohistochemistry
    Sample Tested: Thymus tissue
    Species: Human
    Verified Customer | Posted 03/03/2021
    HIAR Leica ER2 solution primary Ab MAB73091 0.5ug/mL using Leica Bond Refine Detection kit
    Human Helios Antibody MAB73091

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Protocols

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