Human Histamine N‑Methyltransferase/HNMT Antibody

Catalog # Availability Size / Price Qty
AF7637
AF7637-SP
Detection of Human Histamine N-Methyltransferase/HNMT by Western Blot.
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Human Histamine N‑Methyltransferase/HNMT Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histamine N-Methyltransferase/HNMT in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Histamine N-Methyltransferase/HNMT
Ser7-Ala292
Accession # P50135
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Histamine N-Methyltransferase/HNMT by Western Blot. View Larger

Detection of Human Histamine N-Methyltransferase/HNMT by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human stomach tissue, human kidney tissue, and human lung tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Histamine N-Methyltransferase/HNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histamine N-Methyltransferase/HNMT at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine N-Methyltransferase/HNMT

HNMT (Histamine N-MethylTransferase/HMT) is a 32-34 kDa monomeric member of the HNMT family, methyltransferase superfamily of enzymes. It is found in the cytosol of a variety of cell types including visceral smooth muscle cells, bronchial epithelium, erythrocytes and neurons of the central and peripheral nervous system. HNMT is one of two histamine-processing enzymes in mammals. Following transport of histamine into the cell, HNMT catalyzes the addition of adenosyl-L-Met to histamine, generating N-methylhistamine. Human HNMT is 292 amino acids (aa) in length. It contains one methyl transferase domain (aa 32-216) and three potential Ser phosphorylation sites. The HNMT gene is polymorphic, with single aa changes known to impact activity. There are also two splice variants. One shows a five aa substitution for aa 47-292, while another contains a 63 aa substitution for aa 64-292. The latter isoform is reported to possess a GPI-linkage but show no activity on histamine. Over aa 2-292, human HNMT shares 83% aa sequence identity with mouse HNMT.

Long Name
Histamine N-Methyltransferase
Entrez Gene IDs
3176 (Human); 140483 (Mouse); 81676 (Rat)
Alternate Names
EC 2.1.1.8; Histamine NMethyltransferase; Histamine N-Methyltransferase; HMT; HNMT; HNMT-S1; HNMT-S2

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