Human Histamine N‑Methyltransferase/HNMT Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Histamine N-Methyltransferase/HNMT in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Histamine N-Methyltransferase/HNMT
    Ser7-Ala292
    Accession # P50135
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Histamine N-Methyltransferase/HNMT by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human stomach tissue, human kidney tissue, and human lung tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Histamine N-Methyltransferase/HNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histamine N-Methyltransferase/HNMT at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histamine N-Methyltransferase/HNMT

HNMT (Histamine N-MethylTransferase/HMT) is a 32-34 kDa monomeric member of the HNMT family, methyltransferase superfamily of enzymes. It is found in the cytosol of a variety of cell types including visceral smooth muscle cells, bronchial epithelium, erythrocytes and neurons of the central and peripheral nervous system. HNMT is one of two histamine-processing enzymes in mammals. Following transport of histamine into the cell, HNMT catalyzes the addition of adenosyl-L-Met to histamine, generating N-methylhistamine. Human HNMT is 292 amino acids (aa) in length. It contains one methyl transferase domain (aa 32-216) and three potential Ser phosphorylation sites. The HNMT gene is polymorphic, with single aa changes known to impact activity. There are also two splice variants. One shows a five aa substitution for aa 47-292, while another contains a 63 aa substitution for aa 64-292. The latter isoform is reported to possess a GPI-linkage but show no activity on histamine. Over aa 2-292, human HNMT shares 83% aa sequence identity with mouse HNMT.

  • Long Name:
    Histamine N-Methyltransferase
  • Entrez Gene IDs:
    3176 (Human); 140483 (Mouse); 81676 (Rat)
  • Alternate Names:
    EC 2.1.1.8; Histamine NMethyltransferase; Histamine N-Methyltransferase; HMT; HNMT; HNMT-S1; HNMT-S2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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