Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

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Adiponectin/Acrp30 ELISA Kit ELISA
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Product Details
Procedure
Citations (14)
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Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.989 ng/mL
Assay Range
3.9 - 250 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human high molecular weight Adiponectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human HMW Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HMW Adiponectin in serum and plasma. It contains NS0-expressed recombinant human HMW Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HMW Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HMW Adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 25.6 70.6 141 27.7 70.4 134
Standard Deviation 0.67 2.64 3.97 2.4 6 11.1
CV% 2.6 3.7 2.8 8.6 8.5 8.3

Linearity

To assess the linearity of the assay, samples containing high concentrations of HMW Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HMW Adiponectin/Acrp30 ELISA Linearity

Data Examples

Human HMW Adiponectin/Acrp30 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Air pollution and diabetes-related biomarkers in non-diabetic adults: A pathway to impaired glucose metabolism?
    Authors: S Lucht, F Hennig, S Moebus, D Führer-Sak, C Herder, KH Jöckel, B Hoffmann,
    Environ Int, 2019;124(0):370-392.
    Species: Human
    Sample Types:
  2. Analysis of changes on adiponectin levels and abdominal obesity after smoking cessation
    Authors: M Komiyama, H Wada, H Yamakage, N Satoh-Asah, Y Sunagawa, T Morimoto, Y Ozaki, A Shimatsu, Y Takahashi, K Hasegawa
    PLoS ONE, 2018;13(8):e0201244.
    Species: Human
    Sample Types: Serum
  3. Effect of adiposity on tissue-specific adiponectin secretion
    Authors: J Reneau, M Goldblatt, J Gould, T Kindel, A Kastenmeie, R Higgins, LR Rengel, K Schoyer, R James, B Obi, A Moosreiner, K Nicholson, D Sahoo, S Kidambi
    PLoS ONE, 2018;13(6):e0198889.
    Species: Human
    Sample Types: Serum
  4. Serum levels of leptin and high molecular weight adiponectin are inversely associated with radiographic spinal progression in patients with ankylosing spondylitis: results from the ENRADAS trial
    Authors: A Hartl, J Sieper, U Syrbe, J Listing, KG Hermann, M Rudwaleit, D Poddubnyy
    Arthritis Res. Ther., 2017;19(1):140.
    Species: Human
    Sample Types: Serum
  5. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
  6. Alterations in 3-Hydroxyisobutyrate and FGF21 Metabolism are Associated with Protein Ingestion-Induced Insulin Resistance
    Authors: LLS Harris, GI Smith, BW Patterson, RS Ramaswamy, A Okunade, SC Kelly, LC Porter, S Klein, J Yoshino, B Mittendorf
    Diabetes, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  7. Effects of cold sensitivity in the extremities on circulating adiponectin levels and metabolic syndrome in women
    Authors: AY Park, S Cha
    BMC Complement Altern Med, 2017;17(1):150.
    Species: Human
    Sample Types: Serum
  8. Adiponectin, Insulin Sensitivity, ?-Cell Function, and Racial/Ethnic Disparity in Treatment Failure Rates in TODAY
    Diabetes Care, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  9. Gender-Specific Associations between Circulating T-Cadherin and High Molecular Weight-Adiponectin in Patients with Stable Coronary Artery Disease.
    Authors: Schoenenberger A, Pfaff D, Dasen B, Frismantiene A, Erne P, Resink T, Philippova M
    PLoS ONE, 2015;10(6):e0131140.
    Species: Human
    Sample Types: Plasma
  10. Azelnidipine, but not amlodipine, reduces urinary albumin excretion and carotid atherosclerosis in subjects with type 2 diabetes: blood pressure control with olmesartan and azelnidipine in Type 2 diabetes (BOAT2 study).
    Authors: Tawaramoto K, Kaneto H, Hashiramoto M, Kawasaki F, Tatsumi F, Shimoda M, Kamei S, Matsuki M, Mune T, Kaku K
    Diabetol Metab Syndr, 2015;7(0):80.
    Species: Human
    Sample Types: Plasma
  11. Cord blood adipokines, neonatal anthropometrics and postnatal growth in offspring of Hispanic and Native American women with diabetes mellitus.
    Authors: Teague A, Fields D, Aston C, Short K, Lyons T, Chernausek S
    Reprod Biol Endocrinol, 2015;13(0):68.
    Species: Human
    Sample Types: Serum
  12. Adding glimepiride to current insulin therapy increases high-molecular weight adiponectin levels to improve glycemic control in poorly controlled type 2 diabetes.
    Authors: Li C, Zhang J, Yu D, Zhang Q
    Diabetol Metab Syndr, 2014;6(1):41.
    Species: Human
    Sample Types: Plasma
  13. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013;8(12):e83042.
    Species: Human
    Sample Types: Plasma
  14. A prospective study of circulating adipokine levels and risk of multiple myeloma.
    Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
    Blood, 2012;120(22):4418-20.
    Species: Human
    Sample Types: Plasma

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Reviews for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

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Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 11/13/2018
Sample Tested: Adipose tissue

Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 08/03/2017
Sample Tested: Serum

sample dilution used-1:100