Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit

(9 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.989 ng/mL
  • Assay Range
    3.9 - 250 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human high molecular weight Adiponectin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC235, Quantikine Immunoassay Control Group 235 - Please Inquire
Product Summary
The Quantikine Human HMW Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HMW Adiponectin in serum and plasma. It contains NS0-expressed recombinant human HMW Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HMW Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HMW Adiponectin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean25.670.614127.770.4134
Standard Deviation0.672.643.972.4611.1
CV%2.63.72.88.68.58.3


Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean25.670.614127.770.4134
Standard Deviation0.672.643.972.4611.1
CV%2.63.72.88.68.58.3

Linearity
To assess the linearity of the assay, samples containing high concentrations of HMW Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

    • Entrez Gene IDs
      9370 (Human); 11450 (Mouse); 246253 (Rat);
    • Alternate Names
      ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    9 Citations: Showing 1 - 9
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    Species
    Sample Type
    1. Effects of cold sensitivity in the extremities on circulating adiponectin levels and metabolic syndrome in women
      Authors: AY Park, S Cha
      BMC Complement Altern Med, 2017;17(1):150.
      Species: Human
      Sample Type: Serum
    2. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
      Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
      J Immunol Res, 2017;2017(0):6468097.
      Species: Human
      Sample Type: Plasma
    3. Serum levels of leptin and high molecular weight adiponectin are inversely associated with radiographic spinal progression in patients with ankylosing spondylitis: results from the ENRADAS trial
      Authors: A Hartl, J Sieper, U Syrbe, J Listing, KG Hermann, M Rudwaleit, D Poddubnyy
      Arthritis Res. Ther., 2017;19(1):140.
      Species: Human
      Sample Type: Serum
    4. Gender-Specific Associations between Circulating T-Cadherin and High Molecular Weight-Adiponectin in Patients with Stable Coronary Artery Disease.
      Authors: Schoenenberger A, Pfaff D, Dasen B, Frismantiene A, Erne P, Resink T, Philippova M
      PLoS ONE, 2015;10(6):e0131140.
      Species: Human
      Sample Type: Plasma
    5. Azelnidipine, but not amlodipine, reduces urinary albumin excretion and carotid atherosclerosis in subjects with type 2 diabetes: blood pressure control with olmesartan and azelnidipine in Type 2 diabetes (BOAT2 study).
      Authors: Tawaramoto K, Kaneto H, Hashiramoto M, Kawasaki F, Tatsumi F, Shimoda M, Kamei S, Matsuki M, Mune T, Kaku K
      Diabetol Metab Syndr, 2015;7(0):80.
      Species: Human
      Sample Type: Plasma
    6. Cord blood adipokines, neonatal anthropometrics and postnatal growth in offspring of Hispanic and Native American women with diabetes mellitus.
      Authors: Teague A, Fields D, Aston C, Short K, Lyons T, Chernausek S
      Reprod Biol Endocrinol, 2015;13(0):68.
      Species: Human
      Sample Type: Serum
    7. Adding glimepiride to current insulin therapy increases high-molecular weight adiponectin levels to improve glycemic control in poorly controlled type 2 diabetes.
      Authors: Li C, Zhang J, Yu D, Zhang Q
      Diabetol Metab Syndr, 2014;6(1):41.
      Species: Human
      Sample Type: Plasma
    8. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    9. A prospective study of circulating adipokine levels and risk of multiple myeloma.
      Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
      Blood, 2012;120(22):4418-20.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
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    Quantikine Immunoassay Control Group 235

    QC235
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 5 (Based on 1 review)

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     Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit DHWAD0
    : Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit [DHWAD0]

    Summary

    Sample TestedSerum

    Other Experimental Details

    Other Experimental Detailssample dilution used-1:100

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