ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
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Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
R&D Systems | Catalog # DCD540
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Key Product Details
Assay Length
2 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.254 ng/mL
Assay Range
1.56-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
The Quantikine Human sICAM-1 immunoassay is a 2.0 hour solid phase ELISA designed to measure sICAM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human sICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sICAM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human sICAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 4.61 | 12.1 | 19.6 | 4.77 | 12.5 | 20.5 |
| Standard Deviation | 0.17 | 0.63 | 0.98 | 0.32 | 0.67 | 0.90 |
| CV% | 3.7 | 5.2 | 5.0 | 6.7 | 5.4 | 4.4 |
Recovery for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
The recovery of sICAM-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 90-109 |
Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of sICAM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
Human ICAM-1/CD54 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
Product Specific Notices for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
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Sample Tested: EDTA Plasma and EDTA PlasmaVerified Customer | Posted 06/27/2023
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Sample Tested: SerumVerified Customer | Posted 12/06/2021
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Sample Tested: Serum and PlasmaVerified Customer | Posted 10/21/2021Incredibly easy method and good results
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Sample Tested: SerumVerified Customer | Posted 10/14/2021Easy to use, quick assay, good results
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Sample Tested: PlasmaVerified Customer | Posted 02/04/2021Curve developed beautifully, with clear instructions and QC reflecting the sample type.
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Sample Tested: Serum-free Cell Culture SupernatesVerified Customer | Posted 06/08/2020
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Sample Tested: Colon cancer cell lineVerified Customer | Posted 02/15/2020
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 01/13/2020
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Sample Tested: Cell culture supernatantVerified Customer | Posted 12/08/2019
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Sample Tested: HPMECVerified Customer | Posted 09/07/2017
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Protocols
View specific protocols for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit (DCD540):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Conjugate to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Conjugate
100 µL Standard, Control, or Sample
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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