Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540: R&D Systems

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit

(39 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.254 ng/mL
  • Assay Range
    1.6 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human sICAM-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC236, Quantikine Immunoassay Control Group 236 - Please Inquire
Product Summary
The Quantikine Human sICAM-1 immunoassay is a 2.0 hour solid phase ELISA designed to measure sICAM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human sICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sICAM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4.6112.119.64.7712.520.5
Standard Deviation0.170.630.980.320.670.9
CV%3.75.256.75.44.4

Recovery

The recovery of sICAM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 90-109
Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of sICAM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ICAM-1/CD54
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
    • Long Name
      Intercellular Adhesion Molecule 1
    • Entrez Gene IDs
      3383 (Human); 15894 (Mouse); 25464 (Rat);
    • Alternate Names
      BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Conjugate
    4.   Add 100 µL of Conjugate to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Substrate Solution
    9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    10. 50 µL Stop Solution
    11.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    39 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
      Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
      Open Forum Infect Dis, 2016;3(4):ofw224.
      Species: Human
      Sample Type: Plasma
    2. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
      Int J Mol Sci, 2016;17(8):.
      Species: Human
      Sample Type: Serum
    3. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
      Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
      Diabetol Metab Syndr, 2014;6(1):28.
      Species: Human
      Sample Type: Plasma
    4. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    5. The type and quantity of dietary fat and carbohydrate alter faecal microbiome and short-chain fatty acid excretion in a metabolic syndrome 'at-risk' population.
      Authors: Fava, F, Gitau, R, Griffin, B A, Gibson, G R, Tuohy, K M, Lovegrove, J A
      Int J Obes (Lond), 2013;37(2):216-23.
      Species: Human
      Sample Type: Plasma
    6. Biomarker results from the AVADO phase 3 trial of first-line bevacizumab plus docetaxel for HER2-negative metastatic breast cancer.
      Authors: Miles D, de Haas S, Dirix L, Romieu G, Chan A, Pivot X, Tomczak P, Provencher L, Cortes J, Delmar P, Scherer S
      Br J Cancer, 2013;108(5):1052-60.
    7. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
      Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
      Cytokine, 2013;61(3):755-8.
      Species: Human
      Sample Type: Serum
    8. Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting.
      Authors: Lee, Chien-Hs, Shieh, Yi-Shing, Tsai, Chien-Su, Hung, Yi-Jen, Tsai, Yi-Ting, Lin, Chih-Yua
      PLoS ONE, 2013;8(11):e79452.
      Species: Human
      Sample Type: Plasma
    9. Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
      BMC Infect Dis, 2012;12(0):144.
      Species: Human
      Sample Type: Serum
    10. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
      Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
      PLoS ONE, 2012;7(4):e36056.
      Species: Human
      Sample Type: Serum
    11. Suppression of NF-kappaB Reduces Myocardial No-Reflow.
      Authors: Zeng M, Yan H, Chen Y, Zhao H, Lv Y, Liu C, Zhou P, Zhao B
      PLoS ONE, 2012;7(10):e47306.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
      Authors: Tiberti N, Hainard A, Lejon V, Courtioux B, Matovu E, Enyaru JC, Robin X, Turck N, Kristensson K, Ngoyi DM, Vatunga GM, Krishna S, Buscher P, Bisser S, Ndung'u JM, Sanchez JC
      PLoS ONE, 2012;7(7):e40909.
      Species: Human
      Sample Type: CSF
    13. Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
      Authors: Yu SC, Kuo CL, Huang CS, Chang CS, Wu SL, Su SL, Liu CS
      Biomarkers, 2012;17(4):319-24.
      Species: Human
      Sample Type: Plasma
    14. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
      Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
      Int J Obes (Lond), 2011;35(6):800-9.
      Species: Human
      Sample Type: Serum
    15. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
      Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
      Oncol Rep, 2011;26(4):825-31.
      Species: Human
      Sample Type: Serum
    16. Prognostic significance of angiogenic factors in uterine cervical cancer.
      Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2589-95.
      Species: Human
      Sample Type: Serum
    17. Plasma markers for identifying patients with metastatic melanoma.
      Authors: Kluger HM, Hoyt K, Bacchiocchi A, Mayer T, Kirsch J, Kluger Y, Sznol M, Ariyan S, Molinaro A, Halaban R
      Clin. Cancer Res., 2011;17(8):2417-25.
      Species: Human
      Sample Type: Plasma
    18. Influence of a dose-dense adjuvant chemotherapy on sVCAM-1/sICAM-1 serum levels in breast cancer patients with 1-3 positive lymph nodes.
      Authors: Eggeman H, Stoblen F, Thill M, Korlach S, Schmid P, Luftner D, Elling D, Taran FA, Kummel S, Landt S
      Anticancer Res., 2011;31(8):2617-22.
      Species: Human
      Sample Type: Serum
    19. Divergent anti-inflammatory effects of different oil acute consumption on healthy individuals.
      Authors: Papageorgiou N, Tousoulis D, Psaltopoulou T, Giolis A, Antoniades C, Tsiamis E, Miliou A, Toutouzas K, Siasos G, Stefanadis C
      Eur J Clin Nutr, 2011;65(4):514-9.
      Species: Human
      Sample Type: Serum
    20. Evaluation of endothelial function and subclinical atherosclerosis in association with hepatitis C virus in HIV-infected patients: a cross-sectional study.
      Authors: Masia M, Padilla S, Robledano C, Ramos J, Gutierrez F
      BMC Infect Dis, 2011;11(0):265.
      Species: Human
      Sample Type: Plasma
    21. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
      Authors: Hung YJ, Lee CH, Chu NF
      Diabetes Care, 2010;33(8):1840-4.
      Species: Human
      Sample Type: Plasma
    22. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
      Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
      Mol. Cell Proteomics, 2010;9(2):327-35.
      Species: Human
      Sample Type: Plasma
    23. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
      Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
      Am. J. Cardiol., 2010;105(3):362-7.
      Species: Human
      Sample Type: Serum
    24. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    25. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Serum
    26. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
      Authors: Kawut SM, Barr RG, Johnson WC
      Biomarkers, 2010;15(8):731-8.
      Species: Human
      Sample Type: Plasma
    27. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
      Authors: Rhind SG, Crnko NT, Baker AJ
      J Neuroinflammation, 2010;7(0):5.
      Species: Human
      Sample Type: Serum
    28. Cross-sectional relations of multiple inflammatory biomarkers to peripheral arterial disease: The Framingham Offspring Study.
      Authors: Murabito JM, Keyes MJ, Guo CY, Keaney JF, Vasan RS, D'Agostino RB, Benjamin EJ
      Atherosclerosis, 2009;203(2):509-14.
      Species: Human
      Sample Type: Plasma
    29. Non-conventional markers of atherosclerosis before and after gastric banding surgery.
      Authors: Hanusch-Enserer U, Zorn G, Wojta J, Kopp CW, Prager R, Koenig W, Schillinger M, Roden M, Huber K
      Eur. Heart J., 2009;30(12):1516-24.
      Species: Human
      Sample Type: Plasma
    30. Macrophage inflammatory protein-1beta induced cell adhesion with increased intracellular reactive oxygen species.
      Authors: Tatara Y, Ohishi M, Yamamoto K, Shiota A, Hayashi N, Iwamoto Y, Takeda M, Takagi T, Katsuya T, Ogihara T, Rakugi H
      J. Mol. Cell. Cardiol., 2009;47(1):104-11.
      Species: Human
      Sample Type: Plasma
    31. Intensive insulin therapy decreases urinary MCP-1 and ICAM-1 excretions in incipient diabetic nephropathy.
      Authors: Ye SD, Zheng M, Zhao LL, Qian Y, Yao XM, Ren A, Li SM, Jing CY
      Eur. J. Clin. Invest., 2009;39(11):980-5.
      Species: Human
      Sample Type: Urine
    32. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
      Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
      Clin. Sci., 2009;116(10):781-9.
      Species: Human
      Sample Type: Plasma
    33. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
      Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
      Atherosclerosis, 2009;203(2):503-8.
      Species: Human
      Sample Type: Plasma
    34. Consumption of isoflavone-rich soy protein does not alter homocysteine or markers of inflammation in postmenopausal women.
      Authors: Greany KA, Nettleton JA, Wangen KE, Thomas W, Kurzer MS
      Eur J Clin Nutr, 2008;62(0):1419-25.
      Species: Human
      Sample Type: Serum
    35. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
      Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(44):17097-102.
      Species: Human
      Sample Type: Plasma
    36. Association of soluble cell adhesion molecules with ankle-brachial index in a biethnic cohort of predominantly hypertensive individuals.
      Authors: Khaleghi M, Ali Z, Mosley TH, Turner ST, Kullo IJ
      Clin. Chem., 2008;54(11):1788-95.
      Species: Human
      Sample Type: Plasma
    37. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
      Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
      Atherosclerosis, 2007;199(1):215-21.
      Species: Human
      Sample Type: Plasma
    38. Rosiglitazone produces a greater reduction in circulating platelet activity compared with gliclazide in patients with type 2 diabetes mellitus--an effect probably mediated by direct platelet PPARgamma activation.
      Authors: Khanolkar MP, Morris RH, Thomas AW, Bolusani H, Roberts AW, Geen J, Jackson SK, Evans LM
      Atherosclerosis, 2007;197(2):718-24.
      Species: Human
      Sample Type: Serum
    39. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
      Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
      Crit. Care Med., 0;0(0):.
      Species: Human
      Sample Type: Plasma
    Expand to show all 39 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 236

    QC236
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 5 (Based on 1 review)

    Have you used Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (1)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
      Excellent
      Anonymous 09/07/2017
    Show

    Summary

    Sample TestedHPMEC

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information