Human IFN-alpha / beta  R2 C-Terminus Antibody

Catalog # Availability Size / Price Qty
AF7014
AF7014-SP
Detection of Human IFN‑ alpha / beta  R2 by Western Blot.
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Human IFN-alpha / beta  R2 C-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-alpha / beta R2 C-Terminus in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IFN-alpha / beta R2a, rhIFN-alpha / beta R alpha, and recombinant mouse IFN-alpha / beta R2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IFN-alpha / beta R2 C-Terminus
Ser351-Met514
Accession # P48551
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IFN‑ alpha / beta  R2 by Western Blot. View Larger

Detection of Human IFN‑ alpha / beta  R2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, untreated (-) or treated with PMA. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IFN‑ alpha / beta  R2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7014) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN‑ alpha / beta  R2 C-terminus at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 (Interferon alpha/beta receptor 2/IFNAR2; also IFN alpha binding protein) is a 90-102 kDa member of the class II cytokine receptor family of molecules. It is expressed on hematopoietic cells, including T cells, NK cells, B cells and monocytes. It serves as a ligand binding subunit for type I IFN, and generates a functional IFN receptor by complexing with 125 kDa IFNAR1. Multiple type I IFNs signal through this heterodimeric receptor with distinct outcomes. This may be attributable to unique structural conformations created by the Type I IFN:IFNAR1 interaction, or the use of a truncated isoform of IFNAR2. Mature human IFNAR2 is a 489 amino acid (aa) type I transmembrane glycoprotein (aa 27-515). It contains a 217 aa extracellular region (aa 27-243) that contains an Ig-like domain (aa 39-118), a fibronectin type III domain, and a 251 aa cytoplasmic tail. There are two isoform variants. The first is 50-55 kDa in size and contains a 51 aa substitution for aa 281-515. The second is a 40 kDa soluble form that shows a two aa substitution for aa 238-515. IFNAR2 also undergoes proteolysis. Cleavage of the 92 kDa isoform generates a 58 kDa membrane-embedded stub that can be further cleaved into 27-35 kDa fragments that undergo nuclear translocation. Over aa 351-514. human IFNAR2 shares 48% aa identity with mouse IFNAR2.

Long Name
Interferon alpha/beta Receptor 2
Entrez Gene IDs
3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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