Human IFN-gamma Quantikine QuicKit ELISA Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 198 | 1156 | 211 | 1233 |
| Standard Deviation | 3.76 | 20.2 | 20.4 | 56.9 |
| CV% | 1.9 | 1.8 | 9.6 | 4.6 |
Recovery
The recovery of human IFN-gamma spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 92 | 83-102 |
| EDTA Plasma (n=4) | 107 | 88-132 |
| Serum (n=4) | 93 | 74-114 |
Linearity
Scientific Data
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Human IFN-gamma QuicKit ELISA Spiked Recovery Competitor Comparison IFN-gamma is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 115% compared to 144% for the top competitor. Plasma recovery is 120% compared to 135% for the top competitor. Cell Culture Supernates recovery is 118% compared to 79% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
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Human IFN-gamma QuicKit ELISA Spiked Linearity Competitor Comparison IFN-gamma is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-126% compared to 71%-85% for the top competitor.
Product Datasheets
Preparation and Storage
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Citation for Human IFN-gamma Quantikine QuicKit ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Gene expression profiles and bioinformatics analysis in lung samples from ovalbumin-induced asthmatic mice
Authors: Y Song, J Jiang, Q Bai, S Liu, Y Zhang, C Xu, H Piao, L Li, G Yan
BMC pulmonary medicine, 2023-02-02;23(1):50.
Species: Mouse
Sample Types: BALF
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Average Rating: 4 (Based on 1 Review)
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Quick, easy to follow protocol. Urine tested at 2-fold dilution and did not observe a matrix effect.
