Human IGF-I Quantikine ELISA Kit

(50 citations)
(9 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
  • Sensitivity
    0.056 ng/mL
  • Assay Range
    0.1 - 6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IGF-I
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean0.491.2432.3730.3820.9671.941
Standard Deviation0.0170.0540.1030.0310.080.145
CV%3.54.34.38.18.37.5

Recovery

The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 97-102
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IGF-I Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

    • Long Name
      Insulin-like Growth Factor I
    • Entrez Gene IDs
      3479 (Human); 16000 (Mouse); 24482 (Rat);
    • Alternate Names
      IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Assay Diluent
    4.   Add 150 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL cold Conjugate
    9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    50 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
      Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
      Oxid Med Cell Longev, 2017;2017(0):3937842.
      Species: Human
      Sample Type: Plasma
    2. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
      Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
      J Transl Med, 2017;15(1):90.
      Species: Human
      Sample Type: Cell Lysates
    3. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
      Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
      J. Cell. Mol. Med., 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
      Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
      Sci Rep, 2017;7(1):8484.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Metformin inhibits the proliferation of benign prostatic epithelial cells
      Authors: Z Wang, X Xiao, R Ge, J Li, CW Johnson, C Rassoulian, AF Olumi
      PLoS ONE, 2017;12(3):e0173335.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems
      Authors: P Gentile, JP Cole, MA Cole, S Garcovich, A Bielli, MG Scioli, A Orlandi, C Insalaco, V Cervelli
      Int J Mol Sci, 2017;18(2):.
      Species: Human
      Sample Type: Plasma
    7. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
      Stem Cell Res Ther, 2016;7(1):128.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
      Authors: Matthew N Svalina
      Sci Rep, 2016;6(0):27012.
      Species: Human
      Sample Type: CSF
    9. A preliminary study of pamidronic acid downregulation of angiogenic factors IGF-1/PECAM-1 expression in circulating level in bone metastatic breast cancer patients
      Authors: Zeng Wang
      Onco Targets Ther, 2016;9(0):3147-52.
      Species: Human
      Sample Type: Serum
    10. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
      Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
      J Am Heart Assoc, 2016;5(4):.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The tobacco-specific carcinogen-operated calcium channel promotes lung tumorigenesis via IGF2 exocytosis in lung epithelial cells
      Nat Commun, 2016;7(0):12961.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
      PLoS ONE, 2016;11(12):e0168058.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Genetic variation in IGF1 predicts renal cell carcinoma susceptibility and prognosis in Chinese population
      Sci Rep, 2016;6(0):39014.
      Species: Human
      Sample Type: Serum
    14. LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation.
      Authors: Tworkoski K, Raab-Traub N
      J Virol, 2015;89(5):2590-602.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Implantation of rAAV5-IGF-I transduced autologous chondrocytes improves cartilage repair in full-thickness defects in the equine model.
      Authors: Ortved K, Begum L, Mohammed H, Nixon A
      Mol Ther, 2015;23(2):363-73.
      Species: Equine
      Sample Type: Synovial Fluid
    16. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
      Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
      PLoS ONE, 2015;10(2):e0116256.
      Species: Human
      Sample Type: Serum
    17. Overexpression of human IGF-I via direct rAAV-mediated gene transfer improves the early repair of articular cartilage defects in vivo.
      Authors: Cucchiarini M, Madry H
      Gene Ther, 2014;21(9):811-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. A randomized phase II presurgical trial of transdermal 4-hydroxytamoxifen gel versus oral tamoxifen in women with ductal carcinoma in situ of the breast.
      Authors: Lee O, Page K, Ivancic D, Helenowski I, Parini V, Sullivan M, Margenthaler J, Chatterton R, Jovanovic B, Dunn B, Heckman-Stoddard B, Foster K, Muzzio M, Shklovskaya J, Skripkauskas S, Kulesza P, Green D, Hansen N, Bethke K, Jeruss J, Bergan R, Khan S
      Clin Cancer Res, 2014;20(14):3672-82.
      Species: Human
      Sample Type: Plasma
    19. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
      Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
      Stem Cells, 2013;31(1):146-55.
      Species: Human
      Sample Type: Tissue Homogenates
    20. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
      Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
      Cytokine, 2013;61(3):755-8.
      Species: Human
      Sample Type: Serum
    21. Arsenic exposure affects plasma insulin-like growth factor 1 (IGF-1) in children in rural Bangladesh.
      Authors: Ahmed S, Rekha R, Ahsan K, Doi M, Grander M, Roy A, Ekstrom E, Wagatsuma Y, Vahter M, Raqib R
      PLoS ONE, 2013;8(11):e81530.
      Species: Human
      Sample Type: Plasma
    22. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
      Authors: Weimer A, Madry H, Venkatesan JK
      Mol. Med., 2012;18(1):346-58.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
      Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
      PLoS ONE, 2012;7(2):e29164.
      Species: Human
      Sample Type: Plasma
    25. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
      Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
      Br. J. Cancer, 2011;104(1):51-9.
      Species: Human
      Sample Type: Serum
    26. The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
      Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
      PLoS ONE, 2011;6(6):e20533.
      Species: Human
      Sample Type: Serum
    27. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Prognostic significance of angiogenic factors in uterine cervical cancer.
      Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2589-95.
      Species: Human
      Sample Type: Serum
    29. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
      Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
      Obesity (Silver Spring), 2011;19(11):2130-6.
      Species: Human
      Sample Type: Plasma
    30. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    31. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
      Authors: Sesso R, Franco MC
      Am. J. Hypertens., 2010;23(1):6-11.
      Species: Human
      Sample Type: Plasma
    32. Insulin-like growth factor-1 and neurotrophin-3 gene therapy prevents motor decline in an X-linked adrenoleukodystrophy mouse model.
      Authors: Mastroeni R, Bensadoun JC, Charvin D, Aebischer P, Pujol A, Raoul C
      Ann. Neurol., 2009;66(1):117-22.
      Species: Human
      Sample Type: CSF
    33. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
      Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
      Cancer Res., 2009;69(7):2996-3003.
      Species: Human
      Sample Type: Cell Culture Supernates
    34. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Identification of transforming growth factor beta1-driven genetic programs of acute lung fibrosis.
      Authors: Pulichino AM, Wang IM, Caron A, Mortimer J, Auger A, Boie Y, Elias JA, Kartono A, Xu L, Menetski J, Sayegh CE
      Am. J. Respir. Cell Mol. Biol., 2008;39(3):324-36.
      Species: Human
      Sample Type: BALF
    36. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
      Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
      Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
      Species: Human
      Sample Type: Serum
    37. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
      Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
      Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
      Am. J. Respir. Crit. Care Med., 2007;176(3):261-9.
      Species: Human
      Sample Type: Plasma
    39. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
      Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
      Fertil. Steril., 2007;90(1):60-4.
      Species: Human
      Sample Type: Follicular Fluid
    40. Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
      Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
      Fertil. Steril., 2007;88(1):139-44.
      Species: Human
      Sample Type: Serum
    41. Serum concentrations of insulin-like growth factor-I (igf-I) as a marker of liver fibrosis in patients with chronic hepatitis C.
      Authors: Lorenzo-Zuniga V, Bartoli R, Masnou H, Montoliu S, Morillas RM, Planas R
      Dig. Dis. Sci., 2007;52(11):3245-50.
      Species: Human
      Sample Type: Serum
    42. Peripheral elevation of IGF-1 fails to alter Abeta clearance in multiple in vivo models.
      Authors: Lanz TA, Salatto CT, Semproni AR, Marconi M, Brown TM, Richter KE, Schmidt K, Nelson FR, Schachter JB
      Biochem. Pharmacol., 2007;75(5):1093-103.
      Species: Canine
      Sample Type: Plasma
    43. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
      Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
      Clin. Cancer Res., 2007;13(3):1000-9.
      Species: Human
      Sample Type: Plasma
    44. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
      Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
      Fertil. Steril., 2006;86(5):1392-401.
      Species: Human
      Sample Type: Follicular Fluid
    45. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Tissue Homogenates
    46. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
      Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
      Glia, 2006;53(8):845-57.
      Species: Human
      Sample Type: Cell Culture Supernates
    47. Growth hormone-induced stimulation of multilineage human hematopoiesis.
      Authors: Hanley MB, Napolitano LA, McCune JM
      Stem Cells, 2005;23(8):1170-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    48. Insulin growth factor-1 protects against excitotoxicity in the rat striatum.
      Authors: Escartin C, Boyer F, Bemelmans AP, Hantraye P, Brouillet E
      Neuroreport, 2004;15(14):2251-4.
      Species: Human
      Sample Type: Recombinant Protein
    49. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
      Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
      Orthopedics, 2004;27(1):161-5.
    50. Dose response of bone mass to dietary arachidonic acid in piglets fed cow milk-based formula.
      Authors: Blanaru JL, Kohut JR, Fitzpatrick-Wong SC, Weiler HA
      Am. J. Clin. Nutr., 2004;79(1):139-47.
      Species: Porcine
      Sample Type: Plasma
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     Human IGF-I Quantikine ELISA Kit DG100
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      Anonymous 12/01/2017
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    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample TestedEDTA Plasma
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    Sample TestedSerum
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     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample TestedPlasma
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    Sample TestedHuman plasma
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      Anonymous 05/09/2017
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     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample Testedhuman serum
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      Anonymous 11/17/2016
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     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]
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      Anonymous 10/24/2016
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     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample TestedSerum

    Other Experimental Details

    Other Experimental DetailsThe values are always stable.
     Human IGF-I Quantikine ELISA Kit DG100
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     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample TestedCell Culture Supernates
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    : Human IGF-I Quantikine ELISA Kit [DG100]

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    Sample TestedCartilage tissue

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