IGF-1 (IGF-I, insulin-like growth factor-1 or somatomedin C) is a secreted factor that circulates in complex with IGFBP-3 or IGFBP-5 with ALS. It signals through dimeric IGF1R, dimeric Insulin R, or a heterodimer of the two receptors.
Human IGF-I/IGF-1 Quantikine ELISA Kit
R&D Systems | Catalog # DG100
Discontinued Product
DG100 has been discontinued.
An alternative/replacement product is available:
DG100B. View all IGF-I/IGF-1 products.
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
0.056 ng/mL
Assay Range
0.094-6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human IGF-I/IGF-1 Quantikine ELISA Kit
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.490 | 1.243 | 2.373 | 0.382 | 0.967 | 1.941 |
| Standard Deviation | 0.017 | 0.054 | 0.103 | 0.031 | 0.080 | 0.145 |
| CV% | 3.5 | 4.3 | 4.3 | 8.1 | 8.3 | 7.5 |
Recovery for Human IGF-I/IGF-1 Quantikine ELISA Kit
The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 97-102 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IGF-I/IGF-1 Quantikine ELISA Kit
Human IGF-I ELISA Standard Curve
Human IGF-I/IGF-1 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Human IGF-I/IGF-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IGF-I/IGF-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human IGF-I/IGF-1 Quantikine ELISA Kit
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Customer Reviews for Human IGF-I/IGF-1 Quantikine ELISA Kit (14)
4.9 out of 5
14 Customer Ratings
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Sample Tested: Human serumVerified Customer | Posted 06/19/2019
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Sample Tested: PlasmaVerified Customer | Posted 05/24/2019
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Sample Tested: SerumVerified Customer | Posted 01/10/2019
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Sample Tested: SerumVerified Customer | Posted 03/31/2018
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Sample Tested: blood products and Recombinant proteinVerified Customer | Posted 03/22/2018
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Sample Tested: EDTA PlasmaVerified Customer | Posted 12/01/2017
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Sample Tested: SerumVerified Customer | Posted 12/01/2017
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Sample Tested: PlasmaVerified Customer | Posted 09/27/2017
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Sample Tested: Human PlasmaVerified Customer | Posted 05/16/2017
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Sample Tested: Human serumVerified Customer | Posted 05/09/2017
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Verified Customer | Posted 11/17/2016
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Sample Tested: SerumVerified Customer | Posted 10/24/2016The values are always stable.
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 10/14/2016
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Sample Tested: Cartilage tissueVerified Customer | Posted 07/12/2016
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Protocols
View specific protocols for Human IGF-I/IGF-1 Quantikine ELISA Kit (DG100):
Refer to the product for complete assay procedure.
The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 150 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
150 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL cold Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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