Human IGF-I Quantikine ELISA Kit

(33 citations)
(2 Reviews)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
  • Sensitivity
    0.056 ng/mL
  • Assay Range
    0.1 - 6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IGF-I
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0170.0540.1030.0310.080.145


The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 97-102
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IGF-I Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

    • Long Name
      Insulin-like Growth Factor I
    • Entrez Gene IDs
      3479 (Human); 16000 (Mouse); 24482 (Rat);
    • Alternate Names
      IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Assay Diluent
    4.   Add 150 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL cold Conjugate
    9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 33
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    Sample Type
    1. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
      Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
      PLoS ONE, 2015;10(2):e0116256.
      Species: Human
      Sample Type: Serum
    2. A randomized phase II presurgical trial of transdermal 4-hydroxytamoxifen gel versus oral tamoxifen in women with ductal carcinoma in situ of the breast.
      Authors: Lee O, Page K, Ivancic D, Helenowski I, Parini V, Sullivan M, Margenthaler J, Chatterton R, Jovanovic B, Dunn B, Heckman-Stoddard B, Foster K, Muzzio M, Shklovskaya J, Skripkauskas S, Kulesza P, Green D, Hansen N, Bethke K, Jeruss J, Bergan R, Khan S
      Clin Cancer Res, 2014;20(14):3672-82.
      Species: Human
      Sample Type: Plasma
    3. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Arsenic exposure affects plasma insulin-like growth factor 1 (IGF-1) in children in rural Bangladesh.
      Authors: Ahmed S, Rekha R, Ahsan K, Doi M, Grander M, Roy A, Ekstrom E, Wagatsuma Y, Vahter M, Raqib R
      PLoS ONE, 2013;8(11):e81530.
      Species: Human
      Sample Type: Plasma
    5. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
      Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
      Stem Cells, 2013;31(1):146-55.
      Species: Human
      Sample Type: Tissue Lysate
    6. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
      Authors: Weimer A, Madry H, Venkatesan JK
      Mol. Med., 2012;18(1):346-58.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
      Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
      PLoS ONE, 2012;7(2):e29164.
      Species: Human
      Sample Type: Plasma
    8. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    9. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
      Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
      Br. J. Cancer, 2011;104(1):51-9.
      Species: Human
      Sample Type: Serum
    10. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
      Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
      Obesity (Silver Spring), 2011;19(11):2130-6.
      Species: Human
      Sample Type: Plasma
    11. Prognostic significance of angiogenic factors in uterine cervical cancer.
      Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2589-95.
      Species: Human
      Sample Type: Serum
    12. The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
      Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
      PLoS ONE, 2011;6(6):e20533.
      Species: Human
      Sample Type: Serum
    13. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
      Authors: Sesso R, Franco MC
      Am. J. Hypertens., 2010;23(1):6-11.
      Species: Human
      Sample Type: Plasma
    15. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
      Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
      Cancer Res., 2009;69(7):2996-3003.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Insulin-like growth factor-1 and neurotrophin-3 gene therapy prevents motor decline in an X-linked adrenoleukodystrophy mouse model.
      Authors: Mastroeni R, Bensadoun JC, Charvin D, Aebischer P, Pujol A, Raoul C
      Ann. Neurol., 2009;66(1):117-22.
      Species: Human
      Sample Type: CSF
    17. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
      Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Identification of transforming growth factor beta1-driven genetic programs of acute lung fibrosis.
      Authors: Pulichino AM, Wang IM, Caron A, Mortimer J, Auger A, Boie Y, Elias JA, Kartono A, Xu L, Menetski J, Sayegh CE
      Am. J. Respir. Cell Mol. Biol., 2008;39(3):324-36.
      Species: Human
      Sample Type: BALF
    19. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
      Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
      Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
      Species: Human
      Sample Type: Serum
    21. Peripheral elevation of IGF-1 fails to alter Abeta clearance in multiple in vivo models.
      Authors: Lanz TA, Salatto CT, Semproni AR, Marconi M, Brown TM, Richter KE, Schmidt K, Nelson FR, Schachter JB
      Biochem. Pharmacol., 2007;75(5):1093-103.
      Species: Canine
      Sample Type: Plasma
    22. Serum concentrations of insulin-like growth factor-I (igf-I) as a marker of liver fibrosis in patients with chronic hepatitis C.
      Authors: Lorenzo-Zuniga V, Bartoli R, Masnou H, Montoliu S, Morillas RM, Planas R
      Dig. Dis. Sci., 2007;52(11):3245-50.
      Species: Human
      Sample Type: Serum
    23. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
      Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
      Clin. Cancer Res., 2007;13(3):1000-9.
      Species: Human
      Sample Type: Plasma
    24. Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
      Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
      Fertil. Steril., 2007;88(1):139-44.
      Species: Human
      Sample Type: Serum
    25. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
      Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
      Fertil. Steril., 2007;90(1):60-4.
      Species: Human
      Sample Type: Follicular Fluid
    26. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
      Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
      Am. J. Respir. Crit. Care Med., 2007;176(3):261-9.
      Species: Human
      Sample Type: Plasma
    27. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
      Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
      Fertil. Steril., 2006;86(5):1392-401.
      Species: Human
      Sample Type: Follicular Fluid
    28. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Tissue Homogenates
    29. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
      Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
      Glia, 2006;53(8):845-57.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Growth hormone-induced stimulation of multilineage human hematopoiesis.
      Authors: Hanley MB, Napolitano LA, McCune JM
      Stem Cells, 2005;23(8):1170-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
      Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
      Orthopedics, 2004;27(1):161-5.
    32. Dose response of bone mass to dietary arachidonic acid in piglets fed cow milk-based formula.
      Authors: Blanaru JL, Kohut JR, Fitzpatrick-Wong SC, Weiler HA
      Am. J. Clin. Nutr., 2004;79(1):139-47.
      Species: Porcine
      Sample Type: Plasma
    33. Insulin growth factor-1 protects against excitotoxicity in the rat striatum.
      Authors: Escartin C, Boyer F, Bemelmans AP, Hantraye P, Brouillet E
      Neuroreport, 2004;15(14):2251-4.
      Species: Human
      Sample Type: recombinant IGF-I
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    Images Ratings Applications Species Reviewed By Date Details
     Human IGF-I Quantikine ELISA Kit DG100
      Anonymous 10/14/2016
    Image Details
     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]


    Sample TestedCell Culture Supernates
     Human IGF-I Quantikine ELISA Kit DG100
      Anonymous 07/12/2016
    Image Details
     Human IGF-I Quantikine ELISA Kit DG100
    : Human IGF-I Quantikine ELISA Kit [DG100]


    Sample TestedCartilage tissue

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