Human IGFBP-3 Quantikine ELISA Kit

R&D Systems | Catalog # DGB300

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.14 ng/mL

Assay Range

0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human IGFBP-3 Quantikine ELISA Kit

The Quantikine Human IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGFBP-3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IGFBP-3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-3.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IGFBP-3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 4.13 12.7 31.1 4.06 11.8 28.5
Standard Deviation 0.20 0.64 0.71 0.22 0.76 2.27
CV% 4.8 5.0 2.3 5.4 6.4 8.0

Recovery for Human IGFBP-3 Quantikine ELISA Kit

The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGFBP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IGFBP-3 ELISA Linearity

Scientific Data Images for Human IGFBP-3 Quantikine ELISA Kit

Human IGFBP-3 ELISA Standard Curve

Human IGFBP-3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-3

IGFBP-3 (Insulin-like growth factor binding protein-3) modulates the biological activity of IGF. It circulates in a ternary complex with IGF and the acid-labile subunit (ALS). Proteolysis of IGFBP-3 by tissue plasminogen activator (tPA), a disintegrin and metalloproteases (ADAMs), and prostate specific antigen (PSA) contributes to IGFBP-3 degradation or a reduction in its affinity for IGF. The majority of soluble IGFBP-3 found in circulation is secreted from hepatic non-parenchymal cells. IGFBP-3 potentiates EGF-EGFR-mediated cell growth, modulates adipogenesis, and regulates TGF-beta signaling, DNA damage, apoptosis, autophagy, and gene transcription.

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Entrez Gene IDs

3486 (Human); 16009 (Mouse)

Gene Symbol

IGFBP3

Additional IGFBP-3 Products

Product Documents for Human IGFBP-3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IGFBP-3 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IGFBP-3 Quantikine ELISA Kit

Customer Reviews for Human IGFBP-3 Quantikine ELISA Kit (3)

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  • Human IGFBP-3 Quantikine ELISA Kit
    Name: Lilit Baronikian
    Sample Tested: Human Plasma
    Verified Customer | Posted 05/16/2017
  • Human IGFBP-3 Quantikine ELISA Kit
    Name: Lilit Baronikian
    Sample Tested: Human Plasma
    Verified Customer | Posted 05/16/2017
  • Human IGFBP-3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/24/2016
    the results are stable.
    Human IGFBP-3 Quantikine ELISA Kit DGB300

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Protocols

View specific protocols for Human IGFBP-3 Quantikine ELISA Kit (DGB300):

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL cold Conjugate
  6.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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