Human IGFBP-3 Quantikine ELISA Kit

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Human IGFBP-3 ELISA Standard Curve
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Product Details
Procedure
Citations (44)
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Human IGFBP-3 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.14 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IGFBP-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGFBP-3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IGFBP-3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 4.13 12.7 31.1 4.06 11.8 28.5
Standard Deviation 0.2 0.64 0.71 0.22 0.76 2.27
CV% 4.8 5 2.3 5.4 6.4 8

Recovery

The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGFBP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IGFBP-3 ELISA Linearity

Scientific Data

Human IGFBP-3 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name:
Insulin-like Growth Factor Binding Protein 3
Entrez Gene IDs:
3486 (Human); 16009 (Mouse)
Alternate Names:
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IGFBP-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Obese Asthma Phenotype Is Associated with hsa-miR-26a-1-3p and hsa-miR-376a-3p Modulating the IGF Axis
    Authors: Gil-Martínez, M;Lorente-Sorolla, C;Rodrigo-Muñoz, JM;Naharro, S;García-de Castro, Z;Sastre, J;Valverde-Monge, M;Quirce, S;Caballero, ML;Olaguibel, JM;Del Pozo, V;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  2. Selected Parameters of Bone Turnover in Neuroendocrine Tumors-A Potential Clinical Use?
    Authors: Strzelczyk, J;Wójcik-Giertuga, M;Strzelczyk, JK;Seńkowska, AP;Biernacki, K;Kos-Kudła, B;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  3. Energy Homeostasis Gene Nucleotide Variants and Survival of Hemodialysis Patients-A Genetic Cohort Study
    Authors: MK ?widerska, A Mostowska, D Skrypnik, PP Jagodzi?sk, P Bogda?ski, AE Grzegorzew
    Journal of Clinical Medicine, 2022-09-18;11(18):.
    Species: Human
    Sample Types: Plasma
  4. IGFBP-3 Regulates Mitochondrial Hyperfusion and Metabolic Activity in Ocular Surface Epithelia during Hyperosmolar Stress
    Authors: WL Stuard, MK Guner, DM Robertson
    International Journal of Molecular Sciences, 2022-04-06;23(7):.
    Species: Human
    Sample Types: Conditioned Media
  5. Visceral Adiposity, Inflammation, and Testosterone Predict Skeletal Muscle Mitochondrial Mass and Activity in Chronic Spinal Cord Injury
    Authors: JA Goldsmith, RE Lai, RS Garten, Q Chen, EJ Lesnefsky, RA Perera, AS Gorgey
    Frontiers in Physiology, 2022-02-10;13(0):809845.
    Species: Human
    Sample Types: Serum
  6. IGFBP-3 Mediates Metabolic Homeostasis During Hyperosmolar Stress in the Corneal Epithelium
    Authors: ED Bogdan, WL Stuard, R Titone, DM Robertson
    Investigative Ophthalmology & Visual Science, 2021-06-01;62(7):11.
    Species: Human
    Sample Types: Cell Lysates
  7. Discovery and validation of PZP as a novel serum biomarker for screening lung adenocarcinoma in type 2 diabetes mellitus patients
    Authors: J Yang, C Yang, H Shen, W Wu, Z Tian, Q Xu, C Cao, S Ye, L Ban, X Tong, J Mei
    Cancer Cell International, 2021-03-10;21(1):162.
    Species: Human
    Sample Types: Serum
  8. Circulating growth factor concentrations and breast cancer risk: a nested case-control study of IGF-1, IGFBP-3, and breast cancer in a family-based cohort
    Authors: KR Monson, M Goldberg, HC Wu, RM Santella, WK Chung, MB Terry
    Breast Cancer Res, 2020-10-22;22(1):109.
    Species: Human
    Sample Types: Plasma
  9. High intensity interval training combined with L-citrulline supplementation: Effects on physical performance in healthy older adults
    Authors: F Buckinx, LP Carvalho, V Marcangeli, M Dulac, G Hajj Boutr, G Gouspillou, P Gaudreau, P Noirez, M Aubertin-L
    Exp. Gerontol., 2020-07-25;0(0):111036.
    Species: Human
    Sample Types: Serum
  10. Physiological Disturbance in Fatty Liver Energy Metabolism Converges on IGFBP2 Abundance and Regulation in Mice and Men
    Authors: P Fahlbusch, B Knebel, T Hörbelt, DM Barbosa, A Nikolic, S Jacob, H Al-Hasani, F Van de Vel, Y Van Nieuwe, D Müller-Wie, B Lapauw, DM Ouwens, J Kotzka
    Int J Mol Sci, 2020-06-10;21(11):.
    Species: Human
    Sample Types: Serum
  11. PM2.5-bound PAHs exposure linked with low plasma insulin-like growth factor 1 levels and reduced child height
    Authors: Z Zeng, X Huo, Q Wang, C Wang, MN Hylkema, X Xu
    Environ Int, 2020-03-18;138(0):105660.
    Species: Human
    Sample Types: Plasma
  12. Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma
    Authors: J Sakata, A Hirosue, R Yoshida, Y Matsuoka, K Kawahara, H Arita, H Nakashima, T Yamamoto, M Nagata, S Kawaguchi, S Gohara, Y Nagao, K Yamana, R Toya, R Murakami, Y Kuwahara, M Fukumoto, H Nakayama
    Cancers (Basel), 2020-02-20;12(2):.
    Species: Human
    Sample Types: Plasma
  13. Early-Life Cadmium Exposure and Bone-Related Biomarkers: A Longitudinal Study in Children
    Authors: A Malin Igra, M Vahter, R Raqib, M Kippler
    Environ. Health Perspect., 2019-03-01;127(3):37003.
    Species: Human
    Sample Types: Plasma
  14. Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
    Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
    Sci Rep, 2019-02-19;9(1):2304.
    Species: Human
    Sample Types: Plasma
  15. The effects of a combined bodyweight-based and elastic bands resistance training, with or without protein supplementation, on muscle mass, signaling and heat shock response in healthy older people
    Authors: M Krause, D Crognale, K Cogan, S Contarelli, B Egan, P Newsholme, G De Vito
    Exp. Gerontol., 2018-12-05;115(0):104-113.
    Species: Human
    Sample Types: Plasma
  16. Increases in IGF-1 after Anti-TNF-? Therapy are Associated with Bone and Muscle Accrual in Pediatric Crohn's Disease
    Authors: MD DeBoer, AM Lee, K Herbert, J Long, M Thayu, LM Griffin, RN Baldassano, LA Denson, BS Zemel, MR Denburg, R Herskovitz, MB Leonard
    J. Clin. Endocrinol. Metab., 2018-03-01;0(0):.
    Species: Human
    Sample Types: Serum
  17. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
    Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
    Oxid Med Cell Longev, 2017-06-21;2017(0):3937842.
    Species: Human
    Sample Types: Plasma
  18. A phase II randomized, double-blind, presurgical trial of Polyphenon E in bladder cancer patients to evaluate pharmacodynamics and bladder tissue biomarkers
    Authors: JR Gee, DR Saltzstein, K Kim, JM Kolesar, W Huang, TC Havighurst, BW Wollmer, J Stublaski, T Downs, H Mukhtar, M House, HL Parnes, HH Bailey
    Cancer Prev Res (Phila), 2017-03-21;0(0):.
    Species: Human
    Sample Types: Serum
  19. CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect
    Authors: S Sakimoto, V Marchetti, E Aguilar, K Lee, Y Usui, S Murinello, F Bucher, JK Trombley, R Fallon, R Wagey, C Peters, EL Scheppke, PD Westenskow, M Friedlande
    JCI Insight, 2017-01-26;2(2):e89906.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Relationships of insulin-like growth factor-1, its binding proteins, and cardiometabolic risk in hypertensive perimenopausal women
    Authors: A Olszanecka, A Dragan, K Kawecka-Ja, D Fedak, D Czarnecka
    Metab. Clin. Exp., 2017-01-12;69(0):96-106.
    Species: Human
    Sample Types: Plasma
  21. Genetic variation in IGF1 predicts renal cell carcinoma susceptibility and prognosis in Chinese population
    Sci Rep, 2016-12-15;6(0):39014.
    Species: Human
    Sample Types: Serum
  22. Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells
    Authors: Ge Sheng Cheng
    Int. J. Mol. Med, 2016-12-07;39(1):223-230.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Elevations in growth hormone and glucagon-like peptide-2 levels on admission are associated with increased mortality in trauma patients.
    Authors: Matthew P Rowan, Darrick J Beckman, Julie A Rizzo, Claire L Isbell, Christopher E White, Stephen M Cohn, Kevin K Chung
    Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 2016-10-04;0(0):1757-7241.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  24. Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy
    Authors: Howard H Bailey
    Am J Clin Exp Urol, 2016-09-20;4(2):17-27.
    Species: Human
    Sample Types: Serum
  25. The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
    PLoS ONE, 2016-09-01;11(9):e0161500.
    Species: Human
    Sample Types: Serum
  26. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
    Authors: Matthew N Svalina
    Sci Rep, 2016-06-03;6(0):27012.
    Species: Human
    Sample Types: CSF
  27. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
    Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
    PLoS ONE, 2015-02-06;10(2):e0116256.
    Species: Human
    Sample Types: Serum
  28. Glucose intolerance in aging male IGFBP-3 transgenic mice: differential effects of human IGFBP-3 and its mutant IGFBP-3 devoid of IGF binding ability.
    Authors: Nguyen K, Yao X, Erickson A, Mishra S, Nyomba B
    Endocrinology, 2014-12-09;156(2):462-74.
    Species: Human
    Sample Types: Serum
  29. Role of IGF-binding protein 3 in the resistance of EGFR mutant lung cancer cells to EGFR-tyrosine kinase inhibitors.
    Authors: Choi Y, Park G, Rho J, Kim S, So G, Kim H, Choi C, Lee J
    PLoS ONE, 2013-12-05;8(12):e81393.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013-02-14;61(3):755-8.
    Species: Human
    Sample Types: Serum
  31. FOXA1 promotes tumor progression in prostate cancer via the insulin-like growth factor binding protein 3 pathway.
    Authors: Imamura, Yusuke, Sakamoto, Shinichi, Endo, Takumi, Utsumi, Takanobu, Fuse, Miki, Suyama, Takahito, Kawamura, Koji, Imamoto, Takashi, Yano, Kojiro, Uzawa, Katsuhir, Nihei, Naoki, Suzuki, Hiroyosh, Mizokami, Atsushi, Ueda, Takeshi, Seki, Naohiko, Tanzawa, Hideki, Ichikawa, Tomohiko
    PLoS ONE, 2012-08-03;7(8):e42456.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
    Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
    Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Administration of omega-3 fatty acids and Raloxifene to women at high risk of breast cancer: interim feasibility and biomarkers analysis from a clinical trial.
    Eur J Clin Nutr, 2012-06-06;66(8):878-84.
    Species: Human
    Sample Types: Serum
  34. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
    Authors: Weimer A, Madry H, Venkatesan JK
    Mol. Med., 2012-05-09;18(1):346-58.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
    Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
    PLoS ONE, 2012-02-17;7(2):e29164.
    Species: Human
    Sample Types: Plasma
  36. Prognostic significance of angiogenic factors in uterine cervical cancer.
    Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011-08-01;31(8):2589-95.
    Species: Human
    Sample Types: Serum
  37. The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
    Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
    PLoS ONE, 2011-06-02;6(6):e20533.
    Species: Human
    Sample Types: Serum
  38. Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.
    Authors: Oy GF, Slipicevic A, Davidson B, Solberg Faye R, Maelandsmo GM, Florenes VA
    Int. J. Cancer, 2010-01-15;126(2):350-61.
    Species: Human
    Sample Types: Plasma
  39. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
    Authors: Sesso R, Franco MC
    Am. J. Hypertens., 2009-11-05;23(1):6-11.
    Species: Human
    Sample Types: Plasma
  40. Role of insulin-like growth factor binding protein-3 in allergic airway remodeling.
    Authors: Veraldi KL, Gibson BT, Yasuoka H, Myerburg MM, Kelly EA, Balzar S, Jarjour NN, Pilewski JM, Wenzel SE, Feghali-Bostwick CA
    Am. J. Respir. Crit. Care Med., 2009-07-16;180(7):611-7.
    Species: Human
    Sample Types: BALF
  41. Grape seed proanthocyanidins inhibit the growth of human non-small cell lung cancer xenografts by targeting insulin-like growth factor binding protein-3, tumor cell proliferation, and angiogenic factors.
    Authors: Akhtar S, Meeran SM, Katiyar N, Katiyar SK
    Clin. Cancer Res., 2009-02-01;15(3):821-31.
    Species: Human
    Sample Types: Plasma
  42. Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
    Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
    Fertil. Steril., 2007-02-22;88(1):139-44.
    Species: Human
    Sample Types: Serum
  43. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007-02-01;13(3):1000-9.
    Species: Human
    Sample Types: Plasma
  44. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
    Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
    Fertil. Steril., 2006-11-01;86(5):1392-401.
    Species: Human
    Sample Types: Follicular Fluid

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Human IGFBP-3 Quantikine ELISA Kit
By Lilit Baronikian on 05/16/2017
Sample Tested: Human Plasma

Human IGFBP-3 Quantikine ELISA Kit
By Lilit Baronikian on 05/16/2017
Sample Tested: Human Plasma

Human IGFBP-3 Quantikine ELISA Kit
By Anonymous on 10/24/2016
Sample Tested: Serum

the results are stable.