Human IGFBP-3 Quantikine ELISA Kit

  (26 citations)
(3 Reviews)
    
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Assay Procedure
Citations (26)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.14 ng/mL
  • Assay Range
    0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IGFBP-3
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23 , Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGFBP-3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IGFBP-3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-3.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 4.13 12.7 31.1 4.06 11.8 28.5
Standard Deviation 0.2 0.64 0.71 0.22 0.76 2.27
CV% 4.8 5 2.3 5.4 6.4 8

Recovery

The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGFBP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IGFBP-3 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGFBP-3
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 3
  • Entrez Gene IDs:
    3486 (Human); 16009 (Mouse)
  • Alternate Names:
    acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Sample Type
  1. Relationships of insulin-like growth factor-1, its binding proteins, and cardiometabolic risk in hypertensive perimenopausal women
    Authors: A Olszanecka, A Dragan, K Kawecka-Ja, D Fedak, D Czarnecka
    Metab. Clin. Exp., 2017;69(0):96-106.
    Species: Human
    Sample Type: Plasma
  2. Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells
    Authors: Ge Sheng Cheng
    Int. J. Mol. Med, 2017;39(1):223-230.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. A phase II randomized, double-blind, presurgical trial of Polyphenon E in bladder cancer patients to evaluate pharmacodynamics and bladder tissue biomarkers
    Authors: JR Gee, DR Saltzstein, K Kim, JM Kolesar, W Huang, TC Havighurst, BW Wollmer, J Stublaski, T Downs, H Mukhtar, M House, HL Parnes, HH Bailey
    Cancer Prev Res (Phila), 2017;0(0):.
    Species: Human
    Sample Type: Serum
  4. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
    Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
    Oxid Med Cell Longev, 2017;2017(0):3937842.
    Species: Human
    Sample Type: Plasma
  5. CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect
    Authors: S Sakimoto, V Marchetti, E Aguilar, K Lee, Y Usui, S Murinello, F Bucher, JK Trombley, R Fallon, R Wagey, C Peters, EL Scheppke, PD Westenskow, M Friedlande
    JCI Insight, 2017;2(2):e89906.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Genetic variation in IGF1 predicts renal cell carcinoma susceptibility and prognosis in Chinese population
    Sci Rep, 2016;6(0):39014.
    Species: Human
    Sample Type: Serum
  7. The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
    PLoS ONE, 2016;11(9):e0161500.
    Species: Human
    Sample Type: Serum
  8. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
    Authors: Matthew N Svalina
    Sci Rep, 2016;6(0):27012.
    Species: Human
    Sample Type: CSF
  9. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
    Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
    PLoS ONE, 2015;10(2):e0116256.
    Species: Human
    Sample Type: Serum
  10. Glucose intolerance in aging male IGFBP-3 transgenic mice: differential effects of human IGFBP-3 and its mutant IGFBP-3 devoid of IGF binding ability.
    Authors: Nguyen K, Yao X, Erickson A, Mishra S, Nyomba B
    Endocrinology, 2015;156(2):462-74.
    Species: Human
    Sample Type: Serum
  11. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013;61(3):755-8.
    Species: Human
    Sample Type: Serum
  12. Role of IGF-binding protein 3 in the resistance of EGFR mutant lung cancer cells to EGFR-tyrosine kinase inhibitors.
    Authors: Choi Y, Park G, Rho J, Kim S, So G, Kim H, Choi C, Lee J
    PLoS ONE, 2013;8(12):e81393.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. FOXA1 promotes tumor progression in prostate cancer via the insulin-like growth factor binding protein 3 pathway.
    Authors: Imamura, Yusuke, Sakamoto, Shinichi, Endo, Takumi, Utsumi, Takanobu, Fuse, Miki, Suyama, Takahito, Kawamura, Koji, Imamoto, Takashi, Yano, Kojiro, Uzawa, Katsuhir, Nihei, Naoki, Suzuki, Hiroyosh, Mizokami, Atsushi, Ueda, Takeshi, Seki, Naohiko, Tanzawa, Hideki, Ichikawa, Tomohiko
    PLoS ONE, 2012;7(8):e42456.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
    Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
    PLoS ONE, 2012;7(2):e29164.
    Species: Human
    Sample Type: Plasma
  15. Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
    Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
    Proc Natl Acad Sci U S A, 2012;109(30):12052-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
    Authors: Weimer A, Madry H, Venkatesan JK
    Mol. Med., 2012;18(1):346-58.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Administration of omega-3 fatty acids and Raloxifene to women at high risk of breast cancer: interim feasibility and biomarkers analysis from a clinical trial.
    Eur J Clin Nutr, 2012;66(8):878-84.
    Species: Human
    Sample Type: Serum
  18. The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
    Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
    PLoS ONE, 2011;6(6):e20533.
    Species: Human
    Sample Type: Serum
  19. Prognostic significance of angiogenic factors in uterine cervical cancer.
    Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011;31(8):2589-95.
    Species: Human
    Sample Type: Serum
  20. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
    Authors: Sesso R, Franco MC
    Am. J. Hypertens., 2010;23(1):6-11.
    Species: Human
    Sample Type: Plasma
  21. Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.
    Authors: Oy GF, Slipicevic A, Davidson B, Solberg Faye R, Maelandsmo GM, Florenes VA
    Int. J. Cancer, 2010;126(2):350-61.
    Species: Human
    Sample Type: Plasma
  22. Role of insulin-like growth factor binding protein-3 in allergic airway remodeling.
    Authors: Veraldi KL, Gibson BT, Yasuoka H, Myerburg MM, Kelly EA, Balzar S, Jarjour NN, Pilewski JM, Wenzel SE, Feghali-Bostwick CA
    Am. J. Respir. Crit. Care Med., 2009;180(7):611-7.
    Species: Human
    Sample Type: BALF
  23. Grape seed proanthocyanidins inhibit the growth of human non-small cell lung cancer xenografts by targeting insulin-like growth factor binding protein-3, tumor cell proliferation, and angiogenic factors.
    Authors: Akhtar S, Meeran SM, Katiyar N, Katiyar SK
    Clin. Cancer Res., 2009;15(3):821-31.
    Species: Human
    Sample Type: Plasma
  24. Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
    Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
    Fertil. Steril., 2007;88(1):139-44.
    Species: Human
    Sample Type: Serum
  25. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007;13(3):1000-9.
    Species: Human
    Sample Type: Plasma
  26. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
    Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
    Fertil. Steril., 2006;86(5):1392-401.
    Species: Human
    Sample Type: Follicular Fluid
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Average Rating: 5 (Based on 3 reviews)

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 Human IGFBP-3 Quantikine ELISA Kit DGB300 Array DGB300
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 Human IGFBP-3 Quantikine ELISA Kit DGB300
: Human IGFBP-3 Quantikine ELISA Kit [DGB300].

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