Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | ||||||
---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
Mean | 12.6 | 30.4 | 69.2 | 192 | 30.8 | 120 | 194 |
Standard Deviation | 0.3 | 1 | 1.6 | 6.5 | 2.2 | 4.1 | 7.9 |
CV% | 2.4 | 3.3 | 2.3 | 3.4 | 7.1 | 3.4 | 4.1 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | ||||||
---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
Mean | 18.9 | 30.2 | 122 | 181 | 29.8 | 118 | 193 |
Standard Deviation | 1.6 | 1 | 5.4 | 5 | 2.5 | 5 | 8 |
CV% | 8.5 | 3.3 | 4.4 | 2.8 | 8.4 | 4.2 | 4.1 |
Recovery
The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 97 | 80-111 |
Citrate Plasma (n=10) | 93 | 83-110 |
EDTA Plasma (n=10) | 86 | 81-100 |
Heparin Plasma (n=10) | 82 | 76-100 |
Serum (n=10) | 95 | 87-110 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: IL-1 beta/IL-1F2
The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1 alpha, IL-1 beta, and IL-1ra, plus IL-18, IL-33 and IL-1F5-F10. IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions. IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is observed. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples: Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well.
- For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours. - Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.





Citations for Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Tumor-neuroglia interaction promotes pancreatic cancer metastasis
Authors: D Su, X Guo, L Huang, H Ye, Z Li, L Lin, R Chen, Q Zhou
Theranostics, 2020;10(11):5029-5047.
Species: Human
Sample Types: Cell Culture Supernants
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Interleukin-31 interaction with inflammasome: A promising diagnostic and prognostic panel for early sepsis identification in critically ill patients
Authors: MM Watany, MI Elmazny, EM Nasif
Cytokine, 2020;131(0):155102.
Species: Human
Sample Types: Serum
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LINC01116 promotes tumor proliferation and neutrophil recruitment via DDX5-mediated regulation of IL-1&beta in glioma cell
Authors: T Wang, L Cao, X Dong, F Wu, W De, L Huang, Q Wan
Cell Death Dis, 2020;11(5):302.
Species: Human
Sample Types: Cell Culture Supernates
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Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
J Immunol Res, 2020;2020(0):8074183.
Species: Human
Sample Types: Plasma
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Dysfunctional Nurr1 promotes high glucose-induced M�ller cell activation by up-regulating the NF-?B/NLRP3 inflammasome axis
Authors: W Li, X Liu, Y Tu, D Ding, Q Yi, X Sun, Y Wang, K Wang, M Zhu, J Mao
Neuropeptides, 2020;0(0):102057.
Species: Human
Sample Types: Cell Culture Supernates
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Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
Chin. Med. J., 2020;0(6):679-686.
Species: Human
Sample Types: Cell Culture Supernates
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Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease
Authors: N Lalaoui, SE Boyden, H Oda, GM Wood, DL Stone, D Chau, L Liu, M Stoffels, T Kratina, KE Lawlor, KJM Zaal, PM Hoffmann, N Etemadi, K Shield-Art, C Biben, WL Tsai, MD Blake, HS Kuehn, D Yang, H Anderton, N Silke, L Wachsmuth, L Zheng, NS Moura, DB Beck, G Gutierrez-, AK Ombrello, GP Pinto-Pata, AJ Kueh, MJ Herold, C Hall, H Wang, JJ Chae, NI Dmitrieva, M McKenzie, A Light, BK Barham, A Jones, TM Romeo, Q Zhou, I Aksentijev, JC Mullikin, AJ Gross, AK Shum, ED Hawkins, SL Masters, MJ Lenardo, M Boehm, SD Rosenzweig, M Pasparakis, AK Voss, M Gadina, DL Kastner, J Silke
Nature, 2020;577(7788):103-108.
Species: Human
Sample Types: Serum
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Glycated albumin stimulates expression of inflammatory cytokines in muscle cells
Authors: IV Kravchenko, VA Furalyov, VO Popov
Cytokine, 2020;128(0):154991.
Species: Human
Sample Types: Cell Culture Supernates
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Dental pulp cell-derived powerful inducer of TNF-? comprises PKR containing stress granule rich microvesicles
Authors: S Suzuki, T Fukuda, S Nagayasu, J Nakanishi, K Yoshida, S Hirata-Tsu, Y Nakao, T Sano, A Yamashita, S Yamada, K Ohta, H Shiba, F Nishimura
Sci Rep, 2019;9(1):3825.
Species: Human
Sample Types: Cell Culture Supernates
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Genome-wide association study in Turkish and Iranian populations identify rare familial Mediterranean fever gene (MEFV) polymorphisms associated with ankylosing spondylitis
Authors: Z Li, S Akar, H Yarkan, SK Lee, P Çetin, G Can, G Kenar, F Çapa, ON Pamuk, Y Pehlivan, K Cremin, E De Guzman, J Harris, L Wheeler, A Jamshidi, M Vojdanian, E Farhadi, N Ahmadzadeh, Z Yüce, E Dalk?l?ç, D Solmaz, B Ak?n, S Dönmez, ? Sar?, PJ Leo, TJ Kenna, F Önen, M Mahmoudi, MA Brown, N Akkoc
PLoS Genet., 2019;15(4):e1008038.
Species: Human
Sample Types: Serum
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Interplay between pro-inflammatory cytokines, childhood trauma, and executive function in depressed adolescents
Authors: AT Peters, X Ren, KL Bessette, BI Goldstein, AE West, SA Langenecke, GN Pandey
J Psychiatr Res, 2019;114(0):1-10.
Species: Human
Sample Types: Serum
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Sulodexide counteracts endothelial dysfunction induced by metabolic or non-metabolic stresses through activation of the autophagic program
Authors: F De Felice, F Megiorni, I Pietranton, P Tini, G Lessiani, D Mastroiaco, P Mattana, C Antinozzi, L Di Luigi, S Delle Mona, A Angelucci, C Festuccia, A Fanzani, R Maggio, V Tombolini, GL Gravina, F Marampon
Eur Rev Med Pharmacol Sci, 2019;23(6):2669-2680.
Species: Human
Sample Types: Cell Culture Supernates
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Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release
Authors: TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone
PLoS ONE, 2019;14(4):e0214999.
Species: Human
Sample Types: Cell Culture Supernates
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Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in�vitro
Authors: J Li, J Zheng
Mol Med Rep, 2019;19(2):821-830.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of high temperature on pandemic and seasonal human influenza viral replication and infection-induced damage in primary human tracheal epithelial cell cultures
Authors: M Yamaya, H Nishimura, N Lusamba Ka, X Deng, H Momma, Y Shimotai, R Nagatomi
Heliyon, 2019;5(2):e01149.
Species: Human
Sample Types: Cell Culture Supernates
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The Pros1/Tyro3 axis protects against periodontitis by modulating STAT/SOCS signalling
Authors: L Jiang, XQ Chen, MJ Gao, W Lee, J Zhou, YF Zhao, GD Wang
J. Cell. Mol. Med., 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Stimulated hepatic stellate cell promotes progression of hepatocellular carcinoma due to protein kinase R activation
Authors: Y Imai, O Yoshida, T Watanabe, A Yukimoto, Y Koizumi, Y Ikeda, Y Tokumoto, M Hirooka, M Abe, Y Hiasa
PLoS ONE, 2019;14(2):e0212589.
Species: Human
Sample Types: Cell Lysates
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Multilevel defects in the hematopoietic niche in essential thrombocythemia
Authors: T Sun, M Ju, X Dai, H Dong, W Gu, Y Gao, R Fu, X Liu, Y Huang, W Liu, Y Chi, W Wang, H Li, Y Zhou, L Shi, R Yang, L Zhang
Haematologica, 2019;0(0):.
Species: Human
Sample Types: Bone Marrow Fluid
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Downregulated Caveolin-1 expression in circulating monocytes may contribute to the pathogenesis of psoriasis
Authors: N Takamura, Y Yamaguchi, Y Watanabe, M Asami, N Komitsu, M Aihara
Sci Rep, 2019;9(1):125.
Species: Human
Sample Types: Cell Culture Supernates
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Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
Authors: SA Ingelse, J Juschten, MAW Maas, G Matute-Bel, NP Juffermans, JBM van Woense, RA Bem
PLoS ONE, 2019;14(1):e0210172.
Species: Human
Sample Types: Tissue Homogenates
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cAMP metabolism controls caspase-11 inflammasome activation and pyroptosis in sepsis
Authors: R Chen, L Zeng, S Zhu, J Liu, HJ Zeh, G Kroemer, H Wang, TR Billiar, J Jiang, D Tang, R Kang
Sci Adv, 2019;5(5):eaav5562.
Species: Human
Sample Types: Cell Culture Supernates
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Particulate matter induces inflammatory cytokine production via activation of NF?B by TLR5-NOX4-ROS signaling in human skin keratinocyte and mouse skin
Authors: YS Ryu, KA Kang, MJ Piao, MJ Ahn, JM Yi, YM Hyun, SH Kim, MK Ko, CO Park, JW Hyun
Redox Biol, 2019;21(0):101080.
Species: Human
Sample Types: Cell Culture Supernates
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Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
Allergy Asthma Clin Immunol, 2019;15(0):58.
Species: Human
Sample Types: Serum
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Epigenetic downregulation of STAT6 increases HIF-1α expression via mTOR/S6K/S6, leading to enhanced hypoxic viability of glioma cells
Authors: SJ Park, H Kim, SH Kim, EH Joe, I Jou
Acta Neuropathol Commun, 2019;7(1):149.
Species: Human
Sample Types: Cell Culture Supernates
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Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment-colorectal cancer network
Authors: T Ieda, H Tazawa, H Okabayashi, S Yano, K Shigeyasu, S Kuroda, T Ohara, K Noma, H Kishimoto, M Nishizaki, S Kagawa, Y Shirakawa, T Saitou, T Imamura, T Fujiwara
Sci Rep, 2019;9(1):16378.
Species: Human
Sample Types: Cell Culture Supernates
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Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation
Authors: Y He, Q Yang, X Wang, A Jia, W Xie, J Zhou
Mediators Inflamm., 2019;2019(0):5647074.
Species: Human
Sample Types: Plasma
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Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
J. Clin. Invest., 2019;129(7):2792-2806.
Species: Human
Sample Types: Cell Culture Supernates
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Stimulation of Phagocytic Activity in Cultured Human Corneal Fibroblasts by Plasminogen
Authors: T Sato, K Sugioka, A Kodama-Tak, J Murakami, A Saito, H Mishima, T Nishida, S Kusaka
Invest. Ophthalmol. Vis. Sci., 2019;60(13):4205-4214.
Species: Human
Sample Types: Cell Culture Supernates
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Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner
Authors: X Zhang, YS Hwang
Oncol Lett, 2019;18(5):4645-4650.
Species: Human
Sample Types: Cell Culture Supernates
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Evaluation of immunological, inflammatory, and oxidative stress biomarkers in gasoline station attendants
Authors: AM Moro, E Sauer, N Brucker, MF Charão, B Gauer, SN do Nascime, G Goethel, MMMF Duarte, SC Garcia
BMC Pharmacol Toxicol, 2019;20(0):75.
Species: Human
Sample Types: Serum
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Association between body composition and inflammation: A central role of IL-17 and IL-10 in diabetic and hypertensive elderly women
Authors: IS Ribeiro, ÍS Pereira, DP Santos, DS Lopes, AO Prado, SPM Calado, CV Gonçalves, MPL Galantini, IPR Muniz, GS Santos, RAA Silva
Exp. Gerontol., 2019;127(0):110734.
Species: Human
Sample Types: Plasma
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Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome
Authors: H Bai, QF Zhang, JJ Duan, DJ Yu, LJ Liu
Neural Regen Res, 2018;13(12):2147-2155.
Species: Human
Sample Types: Cell Culture Supernates
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Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-?B and IL-1? signal pathways
Authors: Y Wang, J Liu, X Chen, H Sun, S Peng, Y Kuang, J Pi, T Zhuang, L Zhang, Z Yu, B Tomlinson, P Chan, Y Chen, Y Zhang, Y Li
J. Cell. Mol. Med., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Elucidating the Role of CD84 and AHR in Modulation of LPS-Induced Cytokines Production by Cruciferous Vegetable-Derived Compounds Indole-3-Carbinol and 3,3'-Diindolylmethane
Authors: TTY Wang, Q Pham, YS Kim
Int J Mol Sci, 2018;19(2):.
Species: Human
Sample Types: Cell Culture Supernates
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ABCG2 contributes to the development of gout and hyperuricemia in a genome-wide association study
Authors: CJ Chen, CC Tseng, JH Yen, JG Chang, WC Chou, HW Chu, SJ Chang, WT Liao
Sci Rep, 2018;8(1):3137.
Species: Human
Sample Types: Cell Culture Supernates
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Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes
Authors: Y Tanaka, H Uchi, A Hashimoto-, M Furue
Oxid Med Cell Longev, 2018;2018(0):9298052.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-inflammatory cytokine and angiogenic factors levels in vitreous samples of diabetic retinopathy patients
Authors: T Tsai, S Kuehn, N Tsiampalis, MK Vu, V Kakkassery, G Stute, HB Dick, SC Joachim
PLoS ONE, 2018;13(3):e0194603.
Species: Human
Sample Types: Vitreous Humor
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Comparative Analysis of Peptide Composition and Bioactivity of Different Collagen Hydrolysate Batches on Human Osteoarthritic Synoviocytes
Authors: VS Simons, G Lochnit, J Wilhelm, B Ishaque, M Rickert, J Steinmeyer
Sci Rep, 2018;8(1):17733.
Species: Human
Sample Types: Cell Culture Supernates
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Second generation atypical antipsychotics olanzapine and aripiprazole reduce expression and secretion of inflammatory cytokines in human immune cells
Authors: B Stapel, I Sieve, CS Falk, S Bleich, D Hilfiker-K, KG Kahl
J Psychiatr Res, 2018;105(0):95-102.
Species: Human
Sample Types: Cell Culture Supernates
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Aggression, impulsivity and inflammatory markers as risk factors for suicidal behavior
Authors: W Coryell, H Wilcox, SJ Evans, GN Pandey, L Jones-Bran, F Dickerson, R Yolken
J Psychiatr Res, 2018;106(0):38-42.
Species: Human
Sample Types: Plasma
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HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
PLoS ONE, 2018;13(3):e0195335.
Species: Human
Sample Types: Serum
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?-Nicotinamide Adenine Dinucleotide (?-NAD) Inhibits ATP-Dependent IL-1? Release from Human Monocytic Cells
Authors: SD Hiller, S Heldmann, K Richter, I Jurastow, M Küllmar, A Hecker, S Wilker, G Fuchs-Moll, I Manzini, G Schmalzing, W Kummer, W Padberg, JM McIntosh, J Damm, A Zakrzewicz, V Grau
Int J Mol Sci, 2018;19(4):.
Species: Human
Sample Types: Cell Culture Supernates
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Nanoparticle decoration impacts airborne fungal pathobiology
Authors: D Westmeier, D Solouk-Sar, C Vallet, S Siemer, D Docter, H Götz, L Männ, A Hasenberg, A Hahlbrock, K Erler, C Reinhardt, O Schilling, S Becker, M Gunzer, M Hasenberg, SK Knauer, RH Stauber
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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TRAF3-interacting JNK-activating modulator promotes inflammation by stimulating� translocation of Toll-like receptor 4 to lipid rafts
Authors: Y Li, J Guan, W Wang, C Hou, L Zhou, J Ma, Y Cheng, S Jiao, Z Zhou
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Serum
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Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185.
Species: Rat
Sample Types: Serum
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Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
Oncotarget, 2018;9(1):293-305.
Species: Human
Sample Types: Cell Culture Supernates
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Wheat amylase/trypsin inhibitors exacerbate intestinal and airway allergic immune responses in humanized mice
Authors: I Bellinghau, B Weigmann, V Zevallos, J Maxeiner, S Rei beta ig, A Waisman, D Schuppan, J Saloga
J. Allergy Clin. Immunol., 2018;0(0):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Effects of loxoprofen on the apical root resorption during orthodontic tooth movement in rats
Authors: T Yamamoto, M Kaku, H Sumi, Y Yashima, J Izumino, K Tanimoto
PLoS ONE, 2018;13(4):e0194453.
Species: Human
Sample Types: Cell Culture Supernates
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NT-proBNP and CA 125 levels are associated with increased pro-inflammatory cytokines in coronary sinus serum of patients with chronic heart failure
Authors: AE Stanciu, MM Stanciu, RG Vatasescu
Cytokine, 2018;111(0):13-19.
Species: Human
Sample Types: Serum
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Specific Interactions Measured by AFM on Living Cells between Peroxiredoxin-5 and TLR4: Relevance for Mechanisms of Innate Immunity
Authors: B Knoops, S Becker, MA Poncin, J Glibert, S Derclaye, A Clippe, D Alsteens
Cell Chem Biol, 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Adipose-derived stem cells ameliorate colitis by suppression of inflammasome formation and regulation of M1-macrophage population through prostaglandin E2
Authors: HJ Park, J Kim, FT Saima, KJ Rhee, S Hwang, MY Kim, SK Baik, YW Eom, HS Kim
Biochem. Biophys. Res. Commun., 2018;0(0):.
Species: Human
Sample Types: THP-1 cells
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BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity
Authors: RJW Arts, SJCFM Moorlag, B Novakovic, Y Li, SY Wang, M Oosting, V Kumar, RJ Xavier, C Wijmenga, LAB Joosten, CBEM Reusken, CS Benn, P Aaby, MP Koopmans, HG Stunnenber, R van Crevel, MG Netea
Cell Host Microbe, 2018;23(1):89-100.e5.
Species: Human
Sample Types: Cell Culture Supernates
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Does the Use of a "Walking Bleaching" Technique Increase Bone Resorption Markers?
Authors: C Bersezio, P Vildósola, M Sáez, F Sánchez, R Vernal, OB Oliveira, G Jorquera, J Basualdo, A Loguercio, E Fernández
Oper Dent, 2018;0(0):.
Species: Human
Sample Types: Gingival Crevicular Fluid
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Celecoxib-mediated reduction of prostanoid release in Hoffa's fat pad from donors with cartilage pathology results in an attenuated inflammatory phenotype
Authors: UT Timur, MMJ Caron, YM Bastiaanse, TJM Welting, LW van Rhijn, GJVM van Osch, PJ Emans
Osteoarthr. Cartil., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Changes in the Anti-Allergic Activities of Sesame by Bioconversion
Authors: TD Jung, SI Choi, SH Choi, BY Cho, WS Sim,, SJ Lee, SJ Park, DB Kim, YC Kim, JH Lee, OH Lee
Nutrients, 2018;10(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia
Authors: X Yang, J Yan, J Feng
Mol Med Rep, 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Epstein-Barr virus infection-induced inflammasome activation in human monocytes
Authors: Y Torii, JI Kawada, T Murata, H Yoshiyama, H Kimura, Y Ito
PLoS ONE, 2017;12(4):e0175053.
Species: Human
Sample Types: Cell Culture Supernates
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Abnormal gene and protein expression of inflammatory cytokines in the postmortem brain of schizophrenia patients
Authors: GN Pandey, HS Rizavi, H Zhang, X Ren
Schizophr. Res., 2017;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
Dis. Markers, 2017;2017(0):8248175.
Species: Human
Sample Types: Tissue Homogenates
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Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients
Authors: D Heftrig, R Sturm, E Oppermann, K Kontradowi, K Jurida, L Schimunek, M Woschek, I Marzi, B Relja
Mediators Inflamm, 2017;2017(0):2608349.
Species: Human
Sample Types: Complex Sample Type
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A comparison of blood and cerebrospinal fluid cytokines (IL-1?, IL-6, IL-18, TNF-?) in neonates with perinatal hypoxia
Authors: D Šumanovi?-, F ?ulo, MI ?ulo, P Konjevoda, M Jerkovi?-R
Bosn J Basic Med Sci, 2017;0(0):.
Species: Human
Sample Types: Serum
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The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
J Neuroinflammation, 2017;14(1):126.
Species: Human
Sample Types: Serum
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High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
BMC Gastroenterol, 2017;17(1):6.
Species: Human
Sample Types: Serum
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The long non-coding RNA TP73-AS1 modulates HCC cell proliferation through miR-200a-dependent HMGB1/RAGE regulation
Authors: S Li, Y Huang, Y Huang, Y Fu, D Tang, R Kang, R Zhou, XG Fan
J. Exp. Clin. Cancer Res., 2017;36(1):51.
Species: Human
Sample Types: Cell Culture Supernates
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P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
Sci Rep, 2017;7(1):4872.
Species: Human
Sample Types: Tissue Culture Supernates
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Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
Sci Rep, 2017;7(1):16760.
Species: Human
Sample Types: Cell Culture Supernates
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Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1? via Different Combinations of Nicotinic Acetylcholine Receptor Subunits ?7, ?9 and ?10
Authors: A Zakrzewicz, K Richter, A Agné, S Wilker, K Siebers, B Fink, G Krasteva-C, M Althaus, W Padberg, AJ Hone, JM McIntosh, V Grau
Front Cell Neurosci, 2017;11(0):189.
Species: Human
Sample Types: Cell Culture Supernates
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Elastin?derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
Authors: K Kobayashi, R Jokaji, M Miyazawa-H, S Takatsuka, A Tanaka, K Ooi, H Nakamura, S Kawashiri
Mol Med Rep, 2017;16(3):3147-3154.
Species: Human
Sample Types: Synovial Fluid
-
Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells
Authors: T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano
Biochem. Biophys. Res. Commun., 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Stress fibers, autophagy and necrosis by persistent exposure to PM2.5 from biomass combustion
Authors: R Dornhof, C Maschowski, A Osipova, R Gieré, M Seidl, I Merfort, M Humar
PLoS ONE, 2017;12(7):e0180291.
Species: Human
Sample Types: Cell Culture Supernates
-
Simple Plex() : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
Authors: Aldo P, Marusov G, Svancara D, David J, Mor G
Am J Reprod Immunol, 2016;75(6):678-93.
Species: Human
Sample Types: Plasma
-
Pre-treatment serum inflammatory cytokines as survival predictors of patients with nasopharyngeal carcinoma receiving chemoradiotherapy
Authors: AF Al-Kholy, OA Abdullah, MZ Abadier, MM Hassaan, MF Shindy, DM Nor El-Die, A Hasaneen
Mol Clin Oncol, 2016;5(6):811-816.
Species: Human
Sample Types: Serum
-
Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes
Authors: Tatsuya Hasegawa
Biochem Biophys Res Commun, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Cross-talk between intestinal epithelial cells and immune cells in inflammatory bowel disease
Sci Rep, 2016;6(0):29783.
Species: Human
Sample Types: Cell Culture Supernates
-
Clarithromycin Decreases IL-6 Concentration in Serum and BAL Fluid in Patients with Cryptogenic Organizing Pneumonia
Authors: El?bieta Radzikowsk
Adv Clin Exp Med, 2016;25(5):871-878.
Species: Human
Sample Types: Serum
-
Association between mannose-binding lectin variants, haplotypes and risk of hepatocellular carcinoma: A case-control study
Sci Rep, 2016;6(0):32147.
Species: Human
Sample Types: Plasma
-
Simple Plex(�) : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines
Authors: Gil Mor
Am. J. Reprod. Immunol., 2016;75(6):678-93.
Species: Human
Sample Types: Serum
-
Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis
Authors: Qing Li
Sci Rep, 2016;6(0):27566.
Species: Human
Sample Types: Cell Culture Supernates
-
Circulating Fibrocytes Are Increased in Neonates with Bronchopulmonary Dysplasia
Authors: Chun Li
PLoS ONE, 2016;11(6):e0157181.
Species: Human
Sample Types: Plasma
-
Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review
Authors: Z Nazar Maje, K Philip, AM Alabsi, S Pushparaja, D Swaminatha
Dis. Markers, 2016;2016(0):1804727.
Species: Human
Sample Types: Saliva
-
Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
J Cell Mol Med, 2016;0(0):.
Species: Human
Sample Types: Serum
-
A point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains
Authors: S Mairpady S, N Siemens, IR Monk, DB Mohan, S Mukundan, KC Krishnan, S Prabhakara, J Snäll, A Kearns, F Vandenesch, M Svensson, M Kotb, B Gopal, G Arakere, A Norrby-Teg
Sci Rep, 2016;6(0):31360.
Species: Human
Sample Types: Tissue Culture Supernates
-
Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
PLoS ONE, 2016;11(8):e0160153.
Species: Human
Sample Types: Plasma
-
NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
Infect. Immun, 2016;84(9):2422-8.
Species: Human
Sample Types: Cell Culture Supernates
-
The NLRP3 inflammasome and bruton's tyrosine kinase in platelets co-regulate platelet activation, aggregation, and in�vitro thrombus formation
Authors: Pranav Murthy
Biochem. Biophys. Res. Commun, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Glucagon-like peptide 1 improves insulin resistance in vitro through anti-inflammation of macrophages
Authors: X Gu
Braz. J. Med. Biol. Res., 2016;49(12):e5826.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Activation and IL-1? secretion of human peripheral phagocytes infected with Actinomadura madurae, Nocardia asteroides and Candida albicans
Asian Pac J Trop Med, 2016;9(10):962-967.
Species: Human
Sample Types: Cell Culture Supernates
-
Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
PLoS ONE, 2015;10(4):e0121626.
Species: Human
Sample Types: Cell Culture Supernates
-
Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.
Authors: Tokumoto M, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K
Br J Cancer, 2015;113(7):1046-54.
Species: Human
Sample Types: Cell Culture Supernates
-
Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
J Biol Chem, 2015;290(40):24067-78.
Species: Human
Sample Types: Cell Culture Supernates
-
Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
Infect Immun, 2015;83(4):1487-96.
Species: Human
Sample Types: Cell Culture Supernates
-
MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
Authors: Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A
Cancer Res, 2015;75(8):1657-67.
Species: Human
Sample Types: Cell Culture Supernates
-
Phosphocholine-Modified Macromolecules and Canonical Nicotinic Agonists Inhibit ATP-Induced IL-1beta Release.
Authors: Hecker A, Kullmar M, Wilker S, Richter K, Zakrzewicz A, Atanasova S, Mathes V, Timm T, Lerner S, Klein J, Kaufmann A, Bauer S, Padberg W, Kummer W, Janciauskiene S, Fronius M, Schweda E, Lochnit G, Grau V
J Immunol, 2015;195(5):2325-34.
Species: Human
Sample Types: Cell Culture Supernates
-
An Allergic Lung Microenvironment Suppresses Carbon Nanotube-Induced Inflammasome Activation via STAT6-Dependent Inhibition of Caspase-1.
Authors: Shipkowski K, Taylor A, Thompson E, Glista-Baker E, Sayers B, Messenger Z, Bauer R, Jaspers I, Bonner J
PLoS ONE, 2015;10(6):e0128888.
Species: Human
Sample Types: Cell Culture Supernates
-
Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
J Immunol, 2015;194(11):5472-87.
Species: Human
Sample Types: Cell Culture Supernates
-
A novel carboline derivative inhibits nitric oxide formation in macrophages independent of effects on tumor necrosis factor alpha and interleukin-1beta expression.
Authors: Grodzki A, Poola B, Pasupuleti N, Nantz M, Lein P, Gorin F
J Pharmacol Exp Ther, 2015;352(3):438-47.
Species: Human
Sample Types: Cell Culture Supernates
-
Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
PLoS ONE, 2014;9(5):e98517.
Species: Human
Sample Types: Cell Culture Supernates
-
Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
J Diabetes Investig, 2014;5(5):597-605.
Species: Human
Sample Types: Plasma
-
Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
Diabetol Metab Syndr, 2014;6(1):28.
Species: Human
Sample Types: Plasma
-
Adipocyte hypertrophy, inflammation and fibrosis characterize subcutaneous adipose tissue of healthy, non-obese subjects predisposed to type 2 diabetes.
Authors: Henninger A, Eliasson B, Jenndahl L, Hammarstedt A
PLoS ONE, 2014;9(8):e105262.
Species: Human
Sample Types: Serum
-
Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
BMC Biotechnol, 2014;14(0):63.
Species: Human
Sample Types: Serum
-
Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?
Authors: Naik, Pooja, Fofaria, Neel, Prasad, Shikha, Sajja, Ravi K, Weksler, Babette, Couraud, Pierre-O, Romero, Ignacio, Cucullo, Luca
BMC Neurosci, 2014;15(0):51.
Species: Human
Sample Types: Cell Culture Supernates
-
Looking beyond endotoxin: a comparative study of pyrogen retention by ultrafilters used for the preparation of sterile dialyis fluid.
Authors: Glorieux, Griet, Hulko, Michael, Speidel, Rose, Brodbeck, Katrin, Krause, Bernd, Vanholder, Raymond
Sci Rep, 2014;4(0):6390.
Species: Human
Sample Types: Cell Culture Supernates
-
Chlorogenic acid inhibits human platelet activation and thrombus formation.
Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
PLoS ONE, 2014;9(3):e90699.
Species: Human
Sample Types: Cell Culture Supernates
-
Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease.
Authors: Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E
J Clin Invest, 2014;124(9):4115-22.
Species: Human
Sample Types: Plasma
-
beta-glucan induces reactive oxygen species production in human neutrophils to improve the killing of Candida albicans and Candida glabrata isolates from vulvovaginal candidiasis.
Authors: Bonfim-Mendonca P, Ratti B, Godoy J, Negri M, Lima N, Fiorini A, Hatanaka E, Consolaro M, de Oliveira Silva S, Svidzinski T
PLoS ONE, 2014;9(9):e107805.
Species: Human
Sample Types: Cell Culture Supernates
-
Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
PLoS Pathog, 2014;10(8):e1004311.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Tissue Homogenates
-
Preventive effect of Daiokanzoto (TJ-84) on 5-fluorouracil-induced human gingival cell death through the inhibition of reactive oxygen species production.
Authors: Yoshida K, Yoshioka M, Okamura H, Moriyama S, Kawazoe K, Grenier D, Hinode D
PLoS ONE, 2014;9(11):e112689.
Species: Human
Sample Types: Cell Culture Supernates
-
CREB-induced inflammation is important for malignant mesothelioma growth.
Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
Am J Pathol, 2014;184(10):2816-27.
Species: Human
Sample Types: Cell Culture Supernates
-
The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
Authors: Chou C, Lin F, Tsai H, Chang S
BMC Pulm Med, 2014;14(0):182.
Species: Human
Sample Types: BALF
-
Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
PLoS ONE, 2014;9(6):e99612.
Species: Human
Sample Types: Cell Culture Supernates
-
CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
Blood, 2013;121(1):95-106.
Species: Human
Sample Types: Cell Culture Supernates
-
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
J Lipid Res, 2013;54(11):2998-3008.
Species: Human
Sample Types: Cell Culture Supernates
-
Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
Stem Cells, 2013;31(1):146-55.
Species: Human
Sample Types: Tissue Homogenates
-
An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
J Neuroinflammation, 2013;10(0):29.
Species: Human
Sample Types: CSF
-
A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
Acta Pharmacol. Sin., 2012;33(9):1204-16.
Species: Human
Sample Types: Cell Culture Supernates
-
Validation and quality control of ELISAs for the use with human saliva samples.
Authors: Jaedicke KM, Taylor JJ, Preshaw PM
J. Immunol. Methods, 2012;377(1):62-5.
Species: Human
Sample Types: Saliva
-
The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
PLoS ONE, 2012;7(7):e41383.
Species: Human
Sample Types: Cell Culture Supernates
-
High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
J. Bone Miner. Res., 2011;26(9):2082-95.
Species: Human
Sample Types: Cell Culture Supernates
-
IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease.
Authors: Pathak S, Goldofsky E, Vivas EX, Bonagura VR, Vambutas A
J. Immunol., 2011;186(3):1870-9.
Species: Human
Sample Types: Plasma
-
Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
Eur. Respir. J., 2011;38(5):1019-28.
Species: Human
Sample Types: Sputum
-
Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
J. Allergy Clin. Immunol., 2010;126(5):962.
Species: Human
Sample Types: Tissue Homogenates
-
The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
J. Immunol., 2010;185(7):4457-69.
Species: Human
Sample Types: Serum
-
Bacillus anthracis capsule activates caspase-1 and induces interleukin-1beta release from differentiated THP-1 and human monocyte-derived dendritic cells.
Authors: Cho MH, Ahn HJ, Ha HJ, Park J, Chun JH, Kim BS, Oh HB, Rhie GE
Infect. Immun., 2010;78(1):387-92.
Species: Human
Sample Types: Cell Culture Supernates
-
Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
Atherosclerosis, 2009;203(2):503-8.
Species: Human
Sample Types: Plasma
-
Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
J. Immunol., 2009;182(1):547-53.
Species: Human
Sample Types: Cell Culture Supernates
-
Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
J. Exp. Med., 2009;206(3):535-48.
Species: Human
Sample Types: Cell Culture Supernates
-
Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.
Authors: Dossus L, Becker S, Achaintre D, Kaaks R, Rinaldi S
J. Immunol. Methods, 2009;350(1):125-32.
Species: Human
Sample Types: Serum
-
Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
Authors: Chase JC, Celli J, Bosio CM
Infect. Immun., 2009;77(1):180-95.
Species: Human
Sample Types: Cell Culture Supernates
-
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Authors: Semiramoth N, Gleizes A, Turbica I, Sandre C, Gorges R, Kansau I, Servin A, Chollet-Martin S
J. Leukoc. Biol., 2009;85(2):310-21.
Species: Human
Sample Types: Cell Culture Supernates
-
RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
Cancer Res., 2009;69(20):8191-9.
-
Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome.
Authors: Duncan JA, Gao X, Huang MT, O'Connor BP, Thomas CE, Willingham SB, Bergstralh DT, Jarvis GA, Sparling PF, Ting JP
J. Immunol., 2009;182(10):6460-9.
Species: Human
Sample Types: Cell Culture Supernates
-
Characteristics of dengue virus-infected peripheral blood mononuclear cell death that correlates with the severity of illness.
Authors: Jaiyen Y, Masrinoul P, Kalayanarooj S, Pulmanausahakul R, Ubol S
Microbiol. Immunol., 2009;53(8):442-50.
Species: Human
Sample Types: Plasma
-
Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
Clin. Sci., 2009;116(10):781-9.
Species: Human
Sample Types: Plasma
-
Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
Arthritis Rheum., 2008;58(11):3430-5.
Species: Human
Sample Types: Synovial Fluid
-
Decreased lubricin concentrations and markers of joint inflammation in the synovial fluid of patients with anterior cruciate ligament injury.
Authors: Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD
Arthritis Rheum., 2008;58(6):1707-15.
Species: Human
Sample Types: Synovial Fluid
-
The role of nerve growth factor in hyperosmolar stress induced apoptosis.
Authors: Chang EJ, Im YS, Kay EP
J. Cell. Physiol., 2008;216(1):69-77.
Species: Human
Sample Types: Cell Culture Supernates
-
In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
J. Interferon Cytokine Res., 2008;28(5):297-310.
Species: Human
Sample Types: Cell Culture Supernates
-
Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
Anal. Chem., 2008;80(12):4741-51.
Species: Human
Sample Types: Reference Standard
-
Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
Species: Human
Sample Types: BALF
-
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Authors: Rao DA, Tracey KJ, Pober JS
J. Immunol., 2007;179(10):6536-46.
Species: Human
Sample Types: Cell Culture Supernates
-
The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
Fertil. Steril., 2007;90(1):60-4.
Species: Human
Sample Types: Follicular Fluid
-
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
Stem Cells, 2007;26(1):279-89.
Species: Human
Sample Types: Cell Culture Supernates
-
TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
Am. J. Respir. Crit. Care Med., 2007;177(7):763-70.
Species: Human
Sample Types: Whole Cells
-
Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
FEBS Lett., 2007;581(9):1928-32.
Species: Human
Sample Types: Cell Culture Supernates
-
Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
Int. J. Oncol., 2007;31(1):97-102.
Species: Human
Sample Types:
-
C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
Atherosclerosis, 2007;196(1):129-35.
Species: Human
Sample Types: Cell Culture Supernates
-
Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
Int. J. Cancer, 2006;119(10):2313-21.
Species: Human
Sample Types: Cell Culture Supernates
-
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Authors: Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proc. Natl. Acad. Sci. U.S.A., 2006;103(26):9982-7.
Species: Human
Sample Types: Cell Culture Supernates
-
Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
Clin. Cancer Res., 2006;12(7):2055-60.
Species: Human
Sample Types: Plasma
-
Non-esterified fatty acid concentrations are independently associated with hepatic steatosis in obese subjects.
Authors: Holt HB, Wild SH, Wood PJ, Zhang J, Darekar AA, Dewbury K, Poole RB, Holt RI, Phillips DI, Byrne CD
Diabetologia, 2006;49(1):141-8.
Species: Human
Sample Types: Serum
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C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
Arch. Intern. Med., 2006;166(3):366-8.
Species: Human
Sample Types: Plasma
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Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
Authors: Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
J. Immunol., 2006;176(9):5284-92.
Species: Human
Sample Types: Cell Culture Supernates
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The potential of adiponectin in driving arthritis.
Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
J. Immunol., 2006;176(7):4468-78.
Species: Human
Sample Types: Cell Culture Supernates
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Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
Authors: Peterson ML, Schlievert PM
Biochemistry, 2006;45(7):2387-97.
Species: Human
Sample Types: Cell Culture Supernates
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The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
Cell, 2006;124(6):1225-39.
Species: Human
Sample Types: Cell Culture Supernates
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Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells.
Authors: Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P
Cytokine, 2006;36(5):211-7.
Species: Human
Sample Types: Cell Culture Supernates
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Dental plaque, gingival inflammation, and elevated levels of interleukin-6 and cortisol in gingival crevicular fluid from women with stress-related depression and exhaustion.
Authors: Johannsen A, Rylander G, Soder B, Asberg M
J. Periodontol., 2006;77(8):1403-9.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
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Kinin receptor expression during Staphylococcus aureus infection.
Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
Blood, 2006;108(6):2055-63.
Species: Human
Sample Types: Cell Culture Supernates
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The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
Blood, 2006;108(3):947-55.
Species: Human
Sample Types: Cell Culture Supernates
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Humoral and cellular factors responsible for coronary collateral formation.
Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
Am. J. Cardiol., 2006;98(9):1194-7.
Species: Human
Sample Types: Plasma
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Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
J. Orthop. Res., 2005;23(2):397-405.
Species: Human
Sample Types: Synovial Fluid
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Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(3):276-84.
Species: Human
Sample Types: Synovial Fluid
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Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition.
Authors: Witte RP, Kao WJ
Biomaterials, 2005;26(17):3673-82.
Species: Human
Sample Types: Whole Cells
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Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
J. Allergy Clin. Immunol., 2005;116(4):827-35.
Species: Human
Sample Types: Cell Culture Supernates
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Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.
Authors: Bedaiwy MA, Falcone T, Goldberg JM, Attaran M, Sharma R, Miller K, Nelson DR, Agarwal A
J. Assist. Reprod. Genet., 2005;22(4):161-5.
Species: Human
Sample Types: Hypersalpingeal Fluid (HSF)
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Interleukin-1beta tear concentration in glaucomatous and ocular hypertensive patients treated with preservative-free nonselective beta-blockers.
Authors: Manni G, Centofanti M, Oddone F, Parravano M, Bucci MG
Am. J. Ophthalmol., 2005;139(1):72-7.
Species: Human
Sample Types: Tears
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Aseptic peritonitis due to peptidoglycan contamination of pharmacopoeia standard dialysis solution.
Authors: Martis L, Patel M, Giertych J, Mongoven J, Taminne M, Perrier MA, Mendoza O, Goud N, Costigan A, Denjoy N, Verger C, Owen WF
Lancet, 2005;365(9459):588-94.
Species: Human
Sample Types: Complex Sample Type
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Mucosal wound healing in a rabbit model of subglottic stenosis: biochemical analysis of secretions.
Authors: Branski RC, Sandulache VC, Dohar JE, Hebda PA
Arch. Otolaryngol. Head Neck Surg., 2005;131(2):153-7.
Species: Rabbit
Sample Types: Tissue Secretion
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Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
J. Periodont. Res., 2004;39(3):158-67.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
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Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury.
Authors: Hayakata T, Shiozaki T, Tasaki O, Ikegawa H, Inoue Y, Toshiyuki F, Hosotubo H, Kieko F, Yamashita T, Tanaka H, Shimazu T, Sugimoto H
Shock, 2004;22(2):102-7.
Species: Human
Sample Types: Serum
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Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
J. Androl., 2004;25(2):250-4.
Species: Human
Sample Types: Seminal Plasma
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Tissue adhesiveness and host response of in situ photopolymerizable interpenetrating networks containing methylprednisolone acetate.
Authors: Zilinski JL, Kao WJ
J Biomed Mater Res A, 2004;68(2):392-400.
Species: Human
Sample Types: Tissue Perfusion Fluid
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Determinants of plasma leptin concentration in postmenopausal women.
Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
Mech. Ageing Dev., 2004;125(2):117-9.
Species: Human
Sample Types: Whole Cells
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The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases.
Authors: Wei PF, Ho KY, Ho YP, Wu YM, Yang YH, Tsai CC
J. Periodont. Res., 2004;39(5):287-93.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
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Guiding the vaginal microbicide trials with biomarkers of inflammation.
Authors: Fichorova RN
J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
Species: Human
Sample Types: Vaginal Lavage Fluid
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Markers of wound healing in vocal fold secretions from patients with laryngeal pathology.
Authors: Branski RC, Rosen CA, Verdolini K, Hebda PA
Ann. Otol. Rhinol. Laryngol., 2004;113(1):23-9.
Species: Human
Sample Types: Tissue Secretion
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Shifts in biochemical markers associated with wound healing in laryngeal secretions following phonotrauma: a preliminary study.
Authors: Verdolini K, Rosen CA, Branski RC, Hebda PA
Ann. Otol. Rhinol. Laryngol., 2003;112(12):1021-5.
Species: Human
Sample Types: Tissue Secretion
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Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
Int. J. Cancer, 2002;101(2):183-9.
Species: Human
Sample Types: Ascites Fluid
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T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002;110(4):624-33.
Species: Human
Sample Types: Cell Culture Supernates
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Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
Eur. J. Immunol., 2002;32(5):1464-71.
Species: Human
Sample Types: Cell Culture Supernates
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Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
J. Immunol., 2001;167(8):4693-700.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
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Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
Blood, 2001;97(9):2758-63.
Species: Human
Sample Types: Cell Culture Supernates
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Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
Authors: Carstanjen D, Regenfus M, Muller C, Salama A
Exp. Hematol., 2001;29(7):812-21.
Species: Human
Sample Types: Plasma
-
Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins.
Authors: Attur MG, Dave MN, Clancy RM, Patel IR, Abramson SB, Amin AR
J. Immunol., 2000;164(5):2684-91.
Species: Human
Sample Types: Cell Culture Supernates
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Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.
Authors: Welty-Wolf KE, 2016, Carraway MS, Ghio A, Kantrow SP, Huang YC, Piantadosi CA
1-23, 2000;13(5):404-9.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Serum
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Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
J. Exp. Med., 2000;192(2):219-26.
Species: Human
Sample Types: Cell Culture Supernates
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Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis.
Authors: Rasmussen L, Hanstrom L, Lerner UH
J. Clin. Periodontol., 2000;27(1):41-52.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
-
Luminal neutrophil migration in ileoanal pouches studied by whole gut lavage.
Authors: Evgenikos NL, Bartolo DC, Hamer-Hodges DW, Ghosh S
Eur J Gastroenterol Hepatol, 2000;12(5):553-7.
Species: Human
Sample Types: Gut Lavage Fluid
-
Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
Eur. J. Oral Sci., 1998;106(3):778-87.
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
-
Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
J Clin Invest, 1998;101(4):890-8.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
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Does the Human IL-1 beta/IL-1F2 Quantikine® ELISA kit (Catalog # DLB50) detect pro or mature IL-1 beta?
The standard included in the kit is mature recombinant IL-1 beta (p17). This is also the protein used as the immunogen for the antibodies in the kit.
For these reasons, the kit has been designed to measure mature IL-1 beta. In theory, since the same amino acids present in mature IL-1 beta are also present in pro IL-1 beta (p31), it may be possible for the assay to detect pro IL-1 beta, either fully or partially. However, recombinant pro IL-1 beta is not available for R&D Systems to perform cross-reactivity testing.
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Reviews for Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
Average Rating: 4.8 (Based on 10 Reviews)
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Among best ELISAs I've worked with. Great consistency lot to lot, high sensitivity, easy to work with small amount of samples (I've diluted samples 1:100 in the past). Appropriate for concentration response curves. The only thing that can be improved is expanding the dynamic range of the assay (highest standard is at 250pg/ml and in our case we sometimes have 20,000pg/ml so need to make significant dilution to fit within the standard curve).
The Human IL-1beta Quantikine ELISA kit is used for diagnostic use.
Freshly isolated PBMCs from buffy coats stimulated with LPS (1ug/mL) for 3h together with Simvastatin. Total amount of 3 million cells/well. Volume supernatant used: 100uL. Two donors. Great production of IL1B in supernatant. Increased when statins are present as expected. Experiment used as a positive control