Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

  (140 citations)
(7 Reviews)
    
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Assay Procedure
Citations (140)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    1 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC01-1 , Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.

Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3
n 20 20 20 20 20 20 20
Mean 12.6 30.4 69.2 192 30.8 120 194
Standard Deviation 0.3 1 1.6 6.5 2.2 4.1 7.9
CV% 2.4 3.3 2.3 3.4 7.1 3.4 4.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3
n 20 20 20 20 20 20 20
Mean 18.9 30.2 122 181 29.8 118 193
Standard Deviation 1.6 1 5.4 5 2.5 5 8
CV% 8.5 3.3 4.4 2.8 8.4 4.2 4.1

Recovery

The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 80-111
Citrate Plasma (n=10) 93 83-110
EDTA Plasma (n=10) 86 81-100
Heparin Plasma (n=10) 82 76-100
Serum (n=10) 95 87-110
Linearity
To assess linearity of the assay, the following biological samples containing, or spiked with, high concentrations of IL-1 beta were diluted with the appropriate Calibrator Diluent and then assayed.
 IL-1 beta/IL-1F2 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples: Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

140 Citations: Showing 1 - 10
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Species
Sample Type
  1. HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
    Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
    PLoS ONE, 2018;13(3):e0195335.
    Species: Human
    Sample Type: Serum
  2. ?-Nicotinamide Adenine Dinucleotide (?-NAD) Inhibits ATP-Dependent IL-1? Release from Human Monocytic Cells
    Authors: SD Hiller, S Heldmann, K Richter, I Jurastow, M Küllmar, A Hecker, S Wilker, G Fuchs-Moll, I Manzini, G Schmalzing, W Kummer, W Padberg, JM McIntosh, J Damm, A Zakrzewicz, V Grau
    Int J Mol Sci, 2018;19(4):.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Anti-inflammatory cytokine and angiogenic factors levels in vitreous samples of diabetic retinopathy patients
    Authors: T Tsai, S Kuehn, N Tsiampalis, MK Vu, V Kakkassery, G Stute, HB Dick, SC Joachim
    PLoS ONE, 2018;13(3):e0194603.
    Species: Human
    Sample Type: Vitreous Humor
  4. Effects of loxoprofen on the apical root resorption during orthodontic tooth movement in rats
    Authors: T Yamamoto, M Kaku, H Sumi, Y Yashima, J Izumino, K Tanimoto
    PLoS ONE, 2018;13(4):e0194453.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
    Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
    Oncotarget, 2018;9(1):293-305.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. ABCG2 contributes to the development of gout and hyperuricemia in a genome-wide association study
    Authors: CJ Chen, CC Tseng, JH Yen, JG Chang, WC Chou, HW Chu, SJ Chang, WT Liao
    Sci Rep, 2018;8(1):3137.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Elucidating the Role of CD84 and AHR in Modulation of LPS-Induced Cytokines Production by Cruciferous Vegetable-Derived Compounds Indole-3-Carbinol and 3,3'-Diindolylmethane
    Authors: TTY Wang, Q Pham, YS Kim
    Int J Mol Sci, 2018;19(2):.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Elastin?derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
    Authors: K Kobayashi, R Jokaji, M Miyazawa-H, S Takatsuka, A Tanaka, K Ooi, H Nakamura, S Kawashiri
    Mol Med Rep, 2017;16(3):3147-3154.
    Species: Human
    Sample Type: Synovial Fluid
  9. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients
    Authors: D Heftrig, R Sturm, E Oppermann, K Kontradowi, K Jurida, L Schimunek, M Woschek, I Marzi, B Relja
    Mediators Inflamm, 2017;2017(0):2608349.
    Species: Human
    Sample Type: Complex Sample Type
  10. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
    Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
    BMC Gastroenterol, 2017;17(1):6.
    Species: Human
    Sample Type: Serum
  11. The long non-coding RNA TP73-AS1 modulates HCC cell proliferation through miR-200a-dependent HMGB1/RAGE regulation
    Authors: S Li, Y Huang, Y Huang, Y Fu, D Tang, R Kang, R Zhou, XG Fan
    J. Exp. Clin. Cancer Res., 2017;36(1):51.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia
    Authors: X Yang, J Yan, J Feng
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Epstein-Barr virus infection-induced inflammasome activation in human monocytes
    Authors: Y Torii, JI Kawada, T Murata, H Yoshiyama, H Kimura, Y Ito
    PLoS ONE, 2017;12(4):e0175053.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
    Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
    Dis. Markers, 2017;2017(0):8248175.
    Species: Human
    Sample Type: Tissue Homogenates
  15. The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
    Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
    J Neuroinflammation, 2017;14(1):126.
    Species: Human
    Sample Type: Serum
  16. Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1? via Different Combinations of Nicotinic Acetylcholine Receptor Subunits ?7, ?9 and ?10
    Authors: A Zakrzewicz, K Richter, A Agné, S Wilker, K Siebers, B Fink, G Krasteva-C, M Althaus, W Padberg, AJ Hone, JM McIntosh, V Grau
    Front Cell Neurosci, 2017;11(0):189.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Stress fibers, autophagy and necrosis by persistent exposure to PM2.5 from biomass combustion
    Authors: R Dornhof, C Maschowski, A Osipova, R Gieré, M Seidl, I Merfort, M Humar
    PLoS ONE, 2017;12(7):e0180291.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
    Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
    Sci Rep, 2017;7(1):4872.
    Species: Human
    Sample Type: Tissue Culture Supernates
  19. Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
    Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
    Sci Rep, 2017;7(1):16760.
    Species: Human
    Sample Type: Cell Culture Supernate
  20. A comparison of blood and cerebrospinal fluid cytokines (IL-1?, IL-6, IL-18, TNF-?) in neonates with perinatal hypoxia
    Authors: D Šumanovi?-, F ?ulo, MI ?ulo, P Konjevoda, M Jerkovi?-R
    Bosn J Basic Med Sci, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  21. Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells
    Authors: T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis
    Authors: Qing Li
    Sci Rep, 2016;6(0):27566.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. Circulating Fibrocytes Are Increased in Neonates with Bronchopulmonary Dysplasia
    Authors: Chun Li
    PLoS ONE, 2016;11(6):e0157181.
    Species: Human
    Sample Type: Plasma
  24. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
    J Cell Mol Med, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  25. Activation and IL-1? secretion of human peripheral phagocytes infected with Actinomadura madurae, Nocardia asteroides and Candida albicans
    Asian Pac J Trop Med, 2016;9(10):962-967.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes
    Authors: Tatsuya Hasegawa
    Biochem Biophys Res Commun, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review
    Authors: Z Nazar Maje, K Philip, AM Alabsi, S Pushparaja, D Swaminatha
    Dis. Markers, 2016;2016(0):1804727.
    Species: Human
    Sample Type: Saliva
  28. A point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains
    Authors: S Mairpady S, N Siemens, IR Monk, DB Mohan, S Mukundan, KC Krishnan, S Prabhakara, J Snäll, A Kearns, F Vandenesch, M Svensson, M Kotb, B Gopal, G Arakere, A Norrby-Teg
    Sci Rep, 2016;6(0):31360.
    Species: Human
    Sample Type: Tissue Culture Supernates
  29. Association between mannose-binding lectin variants, haplotypes and risk of hepatocellular carcinoma: A case-control study
    Sci Rep, 2016;6(0):32147.
    Species: Human
    Sample Type: Plasma
  30. NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
    Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
    Infect. Immun, 2016;84(9):2422-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  31. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016;11(8):e0160153.
    Species: Human
    Sample Type: Plasma
  32. Simple Plex() : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
    Authors: Aldo P, Marusov G, Svancara D, David J, Mor G
    Am J Reprod Immunol, 2016;75(6):678-93.
    Species: Human
    Sample Type: Plasma
  33. Cross-talk between intestinal epithelial cells and immune cells in inflammatory bowel disease
    Sci Rep, 2016;6(0):29783.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Pre-treatment serum inflammatory cytokines as survival predictors of patients with nasopharyngeal carcinoma receiving chemoradiotherapy
    Authors: AF Al-Kholy, OA Abdullah, MZ Abadier, MM Hassaan, MF Shindy, DM Nor El-Die, A Hasaneen
    Mol Clin Oncol, 2016;5(6):811-816.
    Species: Human
    Sample Type: Serum
  35. The NLRP3 inflammasome and bruton's tyrosine kinase in platelets co-regulate platelet activation, aggregation, and in�vitro thrombus formation
    Authors: Pranav Murthy
    Biochem. Biophys. Res. Commun, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. Clarithromycin Decreases IL-6 Concentration in Serum and BAL Fluid in Patients with Cryptogenic Organizing Pneumonia
    Authors: El?bieta Radzikowsk
    Adv Clin Exp Med, 2016;25(5):871-878.
    Species: Human
    Sample Type: Serum
  37. Phosphocholine-Modified Macromolecules and Canonical Nicotinic Agonists Inhibit ATP-Induced IL-1beta Release.
    Authors: Hecker A, Kullmar M, Wilker S, Richter K, Zakrzewicz A, Atanasova S, Mathes V, Timm T, Lerner S, Klein J, Kaufmann A, Bauer S, Padberg W, Kummer W, Janciauskiene S, Fronius M, Schweda E, Lochnit G, Grau V
    J Immunol, 2015;195(5):2325-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  38. Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.
    Authors: Tokumoto M, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K
    Br J Cancer, 2015;113(7):1046-54.
    Species: Human
    Sample Type: Cell Culture Supernates
  39. MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
    Authors: Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A
    Cancer Res, 2015;75(8):1657-67.
    Species: Human
    Sample Type: Cell Culture Supernates
  40. Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
    Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
    J Biol Chem, 2015;290(40):24067-78.
    Species: Human
    Sample Type: Cell Culture Supernates
  41. An Allergic Lung Microenvironment Suppresses Carbon Nanotube-Induced Inflammasome Activation via STAT6-Dependent Inhibition of Caspase-1.
    Authors: Shipkowski K, Taylor A, Thompson E, Glista-Baker E, Sayers B, Messenger Z, Bauer R, Jaspers I, Bonner J
    PLoS ONE, 2015;10(6):e0128888.
    Species: Human
    Sample Type: Cell Culture Supernates
  42. A novel carboline derivative inhibits nitric oxide formation in macrophages independent of effects on tumor necrosis factor alpha and interleukin-1beta expression.
    Authors: Grodzki A, Poola B, Pasupuleti N, Nantz M, Lein P, Gorin F
    J Pharmacol Exp Ther, 2015;352(3):438-47.
    Species: Human
    Sample Type: Cell Culture Supernates
  43. Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
    Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
    PLoS ONE, 2015;10(4):e0121626.
    Species: Human
    Sample Type: Cell Culture Supernates
  44. Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
    Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
    J Immunol, 2015;194(11):5472-87.
    Species: Human
    Sample Type: Cell Culture Supernates
  45. Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
    Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
    Infect Immun, 2015;83(4):1487-96.
    Species: Human
    Sample Type: Cell Culture Supernates
  46. Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease.
    Authors: Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E
    J Clin Invest, 2014;124(9):4115-22.
    Species: Human
    Sample Type: Plasma
  47. CREB-induced inflammation is important for malignant mesothelioma growth.
    Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
    Am J Pathol, 2014;184(10):2816-27.
    Species: Human
    Sample Type: Cell Culture Supernates
  48. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
    Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
    PLoS ONE, 2014;9(5):e98517.
    Species: Human
    Sample Type: Cell Culture Supernates
  49. Looking beyond endotoxin: a comparative study of pyrogen retention by ultrafilters used for the preparation of sterile dialyis fluid.
    Authors: Glorieux, Griet, Hulko, Michael, Speidel, Rose, Brodbeck, Katrin, Krause, Bernd, Vanholder, Raymond
    Sci Rep, 2014;4(0):6390.
    Species: Human
    Sample Type: Cell Culture Supernates
  50. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
    Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
    Diabetol Metab Syndr, 2014;6(1):28.
    Species: Human
    Sample Type: Plasma
  51. Preventive effect of Daiokanzoto (TJ-84) on 5-fluorouracil-induced human gingival cell death through the inhibition of reactive oxygen species production.
    Authors: Yoshida K, Yoshioka M, Okamura H, Moriyama S, Kawazoe K, Grenier D, Hinode D
    PLoS ONE, 2014;9(11):e112689.
    Species: Human
    Sample Type: Cell Culture Supernates
  52. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014;9(6):e99612.
    Species: Human
    Sample Type: Cell Culture Supernates
  53. Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?
    Authors: Naik, Pooja, Fofaria, Neel, Prasad, Shikha, Sajja, Ravi K, Weksler, Babette, Couraud, Pierre-O, Romero, Ignacio, Cucullo, Luca
    BMC Neurosci, 2014;15(0):51.
    Species: Human
    Sample Type: Cell Culture Supernates
  54. Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
    Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
    J Diabetes Investig, 2014;5(5):597-605.
    Species: Human
    Sample Type: Plasma
  55. Chlorogenic acid inhibits human platelet activation and thrombus formation.
    Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
    PLoS ONE, 2014;9(3):e90699.
    Species: Human
    Sample Type: Cell Culture Supernates
  56. beta-glucan induces reactive oxygen species production in human neutrophils to improve the killing of Candida albicans and Candida glabrata isolates from vulvovaginal candidiasis.
    Authors: Bonfim-Mendonca P, Ratti B, Godoy J, Negri M, Lima N, Fiorini A, Hatanaka E, Consolaro M, de Oliveira Silva S, Svidzinski T
    PLoS ONE, 2014;9(9):e107805.
    Species: Human
    Sample Type: Cell Culture Supernates
  57. Adipocyte hypertrophy, inflammation and fibrosis characterize subcutaneous adipose tissue of healthy, non-obese subjects predisposed to type 2 diabetes.
    Authors: Henninger A, Eliasson B, Jenndahl L, Hammarstedt A
    PLoS ONE, 2014;9(8):e105262.
    Species: Human
    Sample Type: Serum
  58. Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
    Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
    PLoS Pathog, 2014;10(8):e1004311.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Tissue Homogenates
  59. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
    Authors: Chou C, Lin F, Tsai H, Chang S
    BMC Pulm Med, 2014;14(0):182.
    Species: Human
    Sample Type: BALF
  60. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.
    Species: Human
    Sample Type: Serum
  61. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013;10(0):29.
    Species: Human
    Sample Type: CSF
  62. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
    Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
    Stem Cells, 2013;31(1):146-55.
    Species: Human
    Sample Type: Tissue Homogenates
  63. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2013;121(1):95-106.
    Species: Human
    Sample Type: Cell Culture Supernates
  64. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
    Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
    J Lipid Res, 2013;54(11):2998-3008.
    Species: Human
    Sample Type: Cell Culture Supernates
  65. Validation and quality control of ELISAs for the use with human saliva samples.
    Authors: Jaedicke KM, Taylor JJ, Preshaw PM
    J. Immunol. Methods, 2012;377(1):62-5.
    Species: Human
    Sample Type: Saliva
  66. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
    Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
    PLoS ONE, 2012;7(7):e41383.
    Species: Human
    Sample Type: Cell Culture Supernates
  67. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
    Acta Pharmacol. Sin., 2012;33(9):1204-16.
    Species: Human
    Sample Type: Cell Culture Supernates
  68. IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease.
    Authors: Pathak S, Goldofsky E, Vivas EX, Bonagura VR, Vambutas A
    J. Immunol., 2011;186(3):1870-9.
    Species: Human
    Sample Type: Plasma
  69. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
    Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
    Eur. Respir. J., 2011;38(5):1019-28.
    Species: Human
    Sample Type: Sputum
  70. High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
    Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
    J. Bone Miner. Res., 2011;26(9):2082-95.
    Species: Human
    Sample Type: Cell Culture Supernates
  71. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010;126(5):962.
    Species: Human
    Sample Type: Tissue Homogenates
  72. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
    Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
    J. Immunol., 2010;185(7):4457-69.
    Species: Human
    Sample Type: Serum
  73. Bacillus anthracis capsule activates caspase-1 and induces interleukin-1beta release from differentiated THP-1 and human monocyte-derived dendritic cells.
    Authors: Cho MH, Ahn HJ, Ha HJ, Park J, Chun JH, Kim BS, Oh HB, Rhie GE
    Infect. Immun., 2010;78(1):387-92.
    Species: Human
    Sample Type: Cell Culture Supernates
  74. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
    Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
    Atherosclerosis, 2009;203(2):503-8.
    Species: Human
    Sample Type: Plasma
  75. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
    Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
    Clin. Sci., 2009;116(10):781-9.
    Species: Human
    Sample Type: Plasma
  76. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
    Authors: Chase JC, Celli J, Bosio CM
    Infect. Immun., 2009;77(1):180-95.
    Species: Human
    Sample Type: Cell Culture Supernates
  77. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009;206(3):535-48.
    Species: Human
    Sample Type: Cell Culture Supernates
  78. Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
    Authors: Semiramoth N, Gleizes A, Turbica I, Sandre C, Gorges R, Kansau I, Servin A, Chollet-Martin S
    J. Leukoc. Biol., 2009;85(2):310-21.
    Species: Human
    Sample Type: Cell Culture Supernates
  79. Characteristics of dengue virus-infected peripheral blood mononuclear cell death that correlates with the severity of illness.
    Authors: Jaiyen Y, Masrinoul P, Kalayanarooj S, Pulmanausahakul R, Ubol S
    Microbiol. Immunol., 2009;53(8):442-50.
    Species: Human
    Sample Type: Plasma
  80. Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome.
    Authors: Duncan JA, Gao X, Huang MT, O'Connor BP, Thomas CE, Willingham SB, Bergstralh DT, Jarvis GA, Sparling PF, Ting JP
    J. Immunol., 2009;182(10):6460-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  81. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
    Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
    Cancer Res., 2009;69(20):8191-9.
  82. Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
    Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
    J. Immunol., 2009;182(1):547-53.
    Species: Human
    Sample Type: Cell Culture Supernates
  83. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008;28(5):297-310.
    Species: Human
    Sample Type: Cell Culture Supernates
  84. Decreased lubricin concentrations and markers of joint inflammation in the synovial fluid of patients with anterior cruciate ligament injury.
    Authors: Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD
    Arthritis Rheum., 2008;58(6):1707-15.
    Species: Human
    Sample Type: Synovial Fluid
  85. Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
    Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
    Arthritis Rheum., 2008;58(11):3430-5.
    Species: Human
    Sample Type: Synovial Fluid
  86. The role of nerve growth factor in hyperosmolar stress induced apoptosis.
    Authors: Chang EJ, Im YS, Kay EP
    J. Cell. Physiol., 2008;216(1):69-77.
    Species: Human
    Sample Type: Cell Culture Supernates
  87. Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
    Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
    Anal. Chem., 2008;80(12):4741-51.
    Species: Human
    Sample Type: Reference Standard
  88. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
    Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
    FEBS Lett., 2007;581(9):1928-32.
    Species: Human
    Sample Type: Cell Culture Supernates
  89. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
    Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
    Fertil. Steril., 2007;90(1):60-4.
    Species: Human
    Sample Type: Follicular Fluid
  90. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007;179(10):6536-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  91. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
    Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
    Am. J. Respir. Crit. Care Med., 2007;177(7):763-70.
    Species: Human
    Sample Type: Whole Cells
  92. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007;26(1):279-89.
    Species: Human
    Sample Type: Cell Culture Supernates
  93. Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
    Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
    Int. J. Oncol., 2007;31(1):97-102.
    Species: Human
    Sample Type:
  94. Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
    Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
    Species: Human
    Sample Type: BALF
  95. C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
    Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
    Atherosclerosis, 2007;196(1):129-35.
    Species: Human
    Sample Type: Cell Culture Supernates
  96. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
    Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
    Arch. Intern. Med., 2006;166(3):366-8.
    Species: Human
    Sample Type: Plasma
  97. Humoral and cellular factors responsible for coronary collateral formation.
    Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
    Am. J. Cardiol., 2006;98(9):1194-7.
    Species: Human
    Sample Type: Plasma
  98. Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
    Authors: Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
    J. Immunol., 2006;176(9):5284-92.
    Species: Human
    Sample Type: Cell Culture Supernates
  99. Dental plaque, gingival inflammation, and elevated levels of interleukin-6 and cortisol in gingival crevicular fluid from women with stress-related depression and exhaustion.
    Authors: Johannsen A, Rylander G, Soder B, Asberg M
    J. Periodontol., 2006;77(8):1403-9.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  100. Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
    Authors: Peterson ML, Schlievert PM
    Biochemistry, 2006;45(7):2387-97.
    Species: Human
    Sample Type: Cell Culture Supernates
  101. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Type: Cell Culture Supernates
  102. Kinin receptor expression during Staphylococcus aureus infection.
    Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
    Blood, 2006;108(6):2055-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  103. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006;108(3):947-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  104. Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells.
    Authors: Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P
    Cytokine, 2006;36(5):211-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  105. The potential of adiponectin in driving arthritis.
    Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
    J. Immunol., 2006;176(7):4468-78.
    Species: Human
    Sample Type: Cell Culture Supernates
  106. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Type: Cell Culture Supernates
  107. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
    Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
    Clin. Cancer Res., 2006;12(7):2055-60.
    Species: Human
    Sample Type: Plasma
  108. Non-esterified fatty acid concentrations are independently associated with hepatic steatosis in obese subjects.
    Authors: Holt HB, Wild SH, Wood PJ, Zhang J, Darekar AA, Dewbury K, Poole RB, Holt RI, Phillips DI, Byrne CD
    Diabetologia, 2006;49(1):141-8.
    Species: Human
    Sample Type: Serum
  109. The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
    Authors: Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(26):9982-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  110. Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(3):276-84.
    Species: Human
    Sample Type: Synovial Fluid
  111. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
    Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
    J. Orthop. Res., 2005;23(2):397-405.
    Species: Human
    Sample Type: Synovial Fluid
  112. Mucosal wound healing in a rabbit model of subglottic stenosis: biochemical analysis of secretions.
    Authors: Branski RC, Sandulache VC, Dohar JE, Hebda PA
    Arch. Otolaryngol. Head Neck Surg., 2005;131(2):153-7.
    Species: Rabbit
    Sample Type: Tissue Secretion
  113. Interleukin-1beta tear concentration in glaucomatous and ocular hypertensive patients treated with preservative-free nonselective beta-blockers.
    Authors: Manni G, Centofanti M, Oddone F, Parravano M, Bucci MG
    Am. J. Ophthalmol., 2005;139(1):72-7.
    Species: Human
    Sample Type: Tears
  114. Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.
    Authors: Bedaiwy MA, Falcone T, Goldberg JM, Attaran M, Sharma R, Miller K, Nelson DR, Agarwal A
    J. Assist. Reprod. Genet., 2005;22(4):161-5.
    Species: Human
    Sample Type: Hypersalpingeal Fluid (HSF)
  115. Aseptic peritonitis due to peptidoglycan contamination of pharmacopoeia standard dialysis solution.
    Authors: Martis L, Patel M, Giertych J, Mongoven J, Taminne M, Perrier MA, Mendoza O, Goud N, Costigan A, Denjoy N, Verger C, Owen WF
    Lancet, 2005;365(9459):588-94.
    Species: Human
    Sample Type: Complex Sample Type
  116. Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
    Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
    J. Allergy Clin. Immunol., 2005;116(4):827-35.
    Species: Human
    Sample Type: Cell Culture Supernates
  117. Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition.
    Authors: Witte RP, Kao WJ
    Biomaterials, 2005;26(17):3673-82.
    Species: Human
    Sample Type: Whole Cells
  118. Guiding the vaginal microbicide trials with biomarkers of inflammation.
    Authors: Fichorova RN
    J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
    Species: Human
    Sample Type: Vaginal Lavage
  119. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
    Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
    J. Periodont. Res., 2004;39(3):158-67.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  120. Determinants of plasma leptin concentration in postmenopausal women.
    Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
    Mech. Ageing Dev., 2004;125(2):117-9.
    Species: Human
    Sample Type: Whole Cells
  121. The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases.
    Authors: Wei PF, Ho KY, Ho YP, Wu YM, Yang YH, Tsai CC
    J. Periodont. Res., 2004;39(5):287-93.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  122. Tissue adhesiveness and host response of in situ photopolymerizable interpenetrating networks containing methylprednisolone acetate.
    Authors: Zilinski JL, Kao WJ
    J Biomed Mater Res A, 2004;68(2):392-400.
    Species: Human
    Sample Type: Tissue Perfusion Fluid
  123. Markers of wound healing in vocal fold secretions from patients with laryngeal pathology.
    Authors: Branski RC, Rosen CA, Verdolini K, Hebda PA
    Ann. Otol. Rhinol. Laryngol., 2004;113(1):23-9.
    Species: Human
    Sample Type: Tissue Secretion
  124. Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury.
    Authors: Hayakata T, Shiozaki T, Tasaki O, Ikegawa H, Inoue Y, Toshiyuki F, Hosotubo H, Kieko F, Yamashita T, Tanaka H, Shimazu T, Sugimoto H
    Shock, 2004;22(2):102-7.
    Species: Human
    Sample Type: Serum
  125. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004;25(2):250-4.
    Species: Human
    Sample Type: Seminal Plasma
  126. Shifts in biochemical markers associated with wound healing in laryngeal secretions following phonotrauma: a preliminary study.
    Authors: Verdolini K, Rosen CA, Branski RC, Hebda PA
    Ann. Otol. Rhinol. Laryngol., 2003;112(12):1021-5.
    Species: Human
    Sample Type: Tissue Secretion
  127. Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
    Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
    Eur. J. Immunol., 2002;32(5):1464-71.
    Species: Human
    Sample Type: Cell Culture Supernates
  128. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002;101(2):183-9.
    Species: Human
    Sample Type: Ascites Fluid
  129. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
    Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
    J. Allergy Clin. Immunol., 2002;110(4):624-33.
    Species: Human
    Sample Type: Cell Culture Supernates
  130. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
    Authors: Carstanjen D, Regenfus M, Muller C, Salama A
    Exp. Hematol., 2001;29(7):812-21.
    Species: Human
    Sample Type: Plasma
  131. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001;97(9):2758-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  132. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
    Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
    J. Immunol., 2001;167(8):4693-700.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  133. Luminal neutrophil migration in ileoanal pouches studied by whole gut lavage.
    Authors: Evgenikos NL, Bartolo DC, Hamer-Hodges DW, Ghosh S
    Eur J Gastroenterol Hepatol, 2000;12(5):553-7.
    Species: Human
    Sample Type: Gut Lavage Fluid
  134. Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.
    Authors: Welty-Wolf KE, 2016, Carraway MS, Ghio A, Kantrow SP, Huang YC, Piantadosi CA
    1-23, 2000;13(5):404-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Type: Serum
  135. Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis.
    Authors: Rasmussen L, Hanstrom L, Lerner UH
    J. Clin. Periodontol., 2000;27(1):41-52.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  136. Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins.
    Authors: Attur MG, Dave MN, Clancy RM, Patel IR, Abramson SB, Amin AR
    J. Immunol., 2000;164(5):2684-91.
    Species: Human
    Sample Type: Cell Culture Supernates
  137. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
  138. Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
    Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
    Eur. J. Oral Sci., 1998;106(3):778-87.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  139. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
    Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
    J Clin Invest, 1998;101(4):890-8.
    Species: Human
    Sample Type: Cell Culture Supernates
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FAQs

  1. Does the Human IL-1 beta/IL-1F2 Quantikine® ELISA kit (Catalog # DLB50) detect pro or mature IL-1 beta?

    • The standard included in the kit is mature recombinant IL-1 beta (p17). This is also the protein used as the immunogen for the antibodies in the kit.

      For these reasons, the kit has been designed to measure mature IL-1 beta. In theory, since the same amino acids present in mature IL-1 beta are also present in pro IL-1 beta (p31), it may be possible for the assay to detect pro IL-1 beta, either fully or partially. However, recombinant pro IL-1 beta is not available for R&D Systems to perform cross-reactivity testing.

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ELISA Controls
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Quantikine Immunoassay Control Group 1

Ctrl QC01-1  
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Average Rating: 4.9 (Based on 7 reviews)

Have you used Human IL-1 beta/IL-1F2 Quantikine ELISA Kit?

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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Excellent
  Anonymous 05/08/2018
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested Heparin Plasma

Other Experimental Details

Other Experimental Details Among best ELISAs I've worked with. Great consistency lot to lot, high sensitivity, easy to work with small amount of samples (I've diluted samples 1:100 in the past). Appropriate for concentration response curves. The only thing that can be improved is expanding the dynamic range of the assay (highest standard is at 250pg/ml and in our case we sometimes have 20,000pg/ml so need to make significant dilution to fit within the standard curve).
  Excellent
  Anonymous 04/24/2018
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Summary

Sample Tested CHO Chinese hamster ovary cell line
 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Very Good
  Anonymous 04/04/2018
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested Serum and Plasma
 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Excellent
  Anonymous 06/20/2017
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested THP-1 human acute monocytic leukemia cell line
 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Excellent
  Anonymous 04/24/2017
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested Serum

Other Experimental Details

Other Experimental Details The Human IL-1beta Quantikine ELISA kit is used for diagnostic use.
 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Excellent
  Anonymous 03/15/2017
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested Peripheral blood mononuclear cells (PBMCs)

Other Experimental Details

Other Experimental Details Freshly isolated PBMCs from buffy coats stimulated with LPS (1ug/mL) for 3h together with Simvastatin. Total amount of 3 million cells/well. Volume supernatant used: 100uL. Two donors. Great production of IL1B in supernatant. Increased when statins are present as expected. Experiment used as a positive control
 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50 Array DLB50
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Excellent
  Anonymous 04/13/2016
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 Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
: Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50].

Summary

Sample Tested uman plasma

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