Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

(127 citations)
(4 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    1 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.

Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample1234123
n20202020202020
Mean18.930.212218129.8118193
Standard Deviation1.615.452.558
CV%8.53.34.42.88.44.24.1

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample1234123
n20202020202020
Mean12.630.469.219230.8120194
Standard Deviation0.311.66.52.24.17.9
CV%2.43.32.33.47.13.44.1

Recovery

The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 80-111
Citrate Plasma (n=10) 93 83-110
EDTA Plasma (n=10) 86 81-100
Heparin Plasma (n=10) 82 76-100
Serum (n=10) 95 87-110
Linearity
To assess linearity of the assay, the following biological samples containing, or spiked with, high concentrations of IL-1 beta were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
    • Long Name
      Interleukin 1 beta
    • Entrez Gene IDs
      3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig);
    • Alternate Names
      catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent for Serum & Plasma Samples Only
    4.   For Serum & Plasma Samples: Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well.
    10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
      For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    11.   Aspirate and wash 3 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    127 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
      Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
      Sci Rep, 2017;7(1):4872.
      Species: Human
      Sample Type: Tissue Culture Supernates
    2. Epstein-Barr virus infection-induced inflammasome activation in human monocytes
      Authors: Y Torii, JI Kawada, T Murata, H Yoshiyama, H Kimura, Y Ito
      PLoS ONE, 2017;12(4):e0175053.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients
      Authors: D Heftrig, R Sturm, E Oppermann, K Kontradowi, K Jurida, L Schimunek, M Woschek, I Marzi, B Relja
      Mediators Inflamm, 2017;2017(0):2608349.
      Species: Human
      Sample Type: Complex Sample Type
    4. The long non-coding RNA TP73-AS1 modulates HCC cell proliferation through miR-200a-dependent HMGB1/RAGE regulation
      Authors: S Li, Y Huang, Y Huang, Y Fu, D Tang, R Kang, R Zhou, XG Fan
      J. Exp. Clin. Cancer Res., 2017;36(1):51.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia
      Authors: X Yang, J Yan, J Feng
      Mol Med Rep, 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
      Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
      BMC Gastroenterol, 2017;17(1):6.
      Species: Human
      Sample Type: Serum
    7. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
      Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
      Dis. Markers, 2017;2017(0):8248175.
      Species: Human
      Sample Type: Tissue Homogenates
    8. The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
      Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
      J Neuroinflammation, 2017;14(1):126.
      Species: Human
      Sample Type: Serum
    9. Elastin?derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
      Authors: K Kobayashi, R Jokaji, M Miyazawa-H, S Takatsuka, A Tanaka, K Ooi, H Nakamura, S Kawashiri
      Mol Med Rep, 2017;16(3):3147-3154.
      Species: Human
      Sample Type: Synovial Fluid
    10. Stress fibers, autophagy and necrosis by persistent exposure to PM2.5 from biomass combustion
      Authors: R Dornhof, C Maschowski, A Osipova, R Gieré, M Seidl, I Merfort, M Humar
      PLoS ONE, 2017;12(7):e0180291.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1? via Different Combinations of Nicotinic Acetylcholine Receptor Subunits ?7, ?9 and ?10
      Authors: A Zakrzewicz, K Richter, A Agné, S Wilker, K Siebers, B Fink, G Krasteva-C, M Althaus, W Padberg, AJ Hone, JM McIntosh, V Grau
      Front Cell Neurosci, 2017;11(0):189.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis
      Authors: Qing Li
      Sci Rep, 2016;6(0):27566.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes
      Authors: Tatsuya Hasegawa
      Biochem Biophys Res Commun, 2016;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Cross-talk between intestinal epithelial cells and immune cells in inflammatory bowel disease
      Sci Rep, 2016;6(0):29783.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
      PLoS ONE, 2016;11(8):e0160153.
      Species: Human
      Sample Type: Plasma
    16. Association between mannose-binding lectin variants, haplotypes and risk of hepatocellular carcinoma: A case-control study
      Sci Rep, 2016;6(0):32147.
      Species: Human
      Sample Type: Plasma
    17. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
      J Cell Mol Med, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    18. Circulating Fibrocytes Are Increased in Neonates with Bronchopulmonary Dysplasia
      Authors: Chun Li
      PLoS ONE, 2016;11(6):e0157181.
      Species: Human
      Sample Type: Plasma
    19. Activation and IL-1? secretion of human peripheral phagocytes infected with Actinomadura madurae, Nocardia asteroides and Candida albicans
      Asian Pac J Trop Med, 2016;9(10):962-967.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Simple Plex() : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
      Authors: Aldo P, Marusov G, Svancara D, David J, Mor G
      Am J Reprod Immunol, 2016;75(6):678-93.
      Species: Human
      Sample Type: Plasma
    21. Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review
      Authors: Z Nazar Maje, K Philip, AM Alabsi, S Pushparaja, D Swaminatha
      Dis. Markers, 2016;2016(0):1804727.
      Species: Human
      Sample Type: Saliva
    22. A point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains
      Authors: S Mairpady S, N Siemens, IR Monk, DB Mohan, S Mukundan, KC Krishnan, S Prabhakara, J Snäll, A Kearns, F Vandenesch, M Svensson, M Kotb, B Gopal, G Arakere, A Norrby-Teg
      Sci Rep, 2016;6(0):31360.
      Species: Human
      Sample Type: Tissue Culture Supernates
    23. NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
      Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
      Infect. Immun, 2016;84(9):2422-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Phosphocholine-Modified Macromolecules and Canonical Nicotinic Agonists Inhibit ATP-Induced IL-1beta Release.
      Authors: Hecker A, Kullmar M, Wilker S, Richter K, Zakrzewicz A, Atanasova S, Mathes V, Timm T, Lerner S, Klein J, Kaufmann A, Bauer S, Padberg W, Kummer W, Janciauskiene S, Fronius M, Schweda E, Lochnit G, Grau V
      J Immunol, 2015;195(5):2325-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. A novel carboline derivative inhibits nitric oxide formation in macrophages independent of effects on tumor necrosis factor alpha and interleukin-1beta expression.
      Authors: Grodzki A, Poola B, Pasupuleti N, Nantz M, Lein P, Gorin F
      J Pharmacol Exp Ther, 2015;352(3):438-47.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
      Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
      PLoS ONE, 2015;10(4):e0121626.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
      Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
      J Immunol, 2015;194(11):5472-87.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
      Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
      Infect Immun, 2015;83(4):1487-96.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. An Allergic Lung Microenvironment Suppresses Carbon Nanotube-Induced Inflammasome Activation via STAT6-Dependent Inhibition of Caspase-1.
      Authors: Shipkowski K, Taylor A, Thompson E, Glista-Baker E, Sayers B, Messenger Z, Bauer R, Jaspers I, Bonner J
      PLoS ONE, 2015;10(6):e0128888.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
      Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
      J Biol Chem, 2015;290(40):24067-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
      Authors: Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A
      Cancer Res, 2015;75(8):1657-67.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.
      Authors: Tokumoto M, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K
      Br J Cancer, 2015;113(7):1046-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease.
      Authors: Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E
      J Clin Invest, 2014;124(9):4115-22.
      Species: Human
      Sample Type: Plasma
    34. Adipocyte hypertrophy, inflammation and fibrosis characterize subcutaneous adipose tissue of healthy, non-obese subjects predisposed to type 2 diabetes.
      Authors: Henninger A, Eliasson B, Jenndahl L, Hammarstedt A
      PLoS ONE, 2014;9(8):e105262.
      Species: Human
      Sample Type: Serum
    35. Chlorogenic acid inhibits human platelet activation and thrombus formation.
      Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
      PLoS ONE, 2014;9(3):e90699.
      Species: Human
      Sample Type: Cell Culture Supernates
    36. beta-glucan induces reactive oxygen species production in human neutrophils to improve the killing of Candida albicans and Candida glabrata isolates from vulvovaginal candidiasis.
      Authors: Bonfim-Mendonca P, Ratti B, Godoy J, Negri M, Lima N, Fiorini A, Hatanaka E, Consolaro M, de Oliveira Silva S, Svidzinski T
      PLoS ONE, 2014;9(9):e107805.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Preventive effect of Daiokanzoto (TJ-84) on 5-fluorouracil-induced human gingival cell death through the inhibition of reactive oxygen species production.
      Authors: Yoshida K, Yoshioka M, Okamura H, Moriyama S, Kawazoe K, Grenier D, Hinode D
      PLoS ONE, 2014;9(11):e112689.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
      Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
      J Diabetes Investig, 2014;5(5):597-605.
      Species: Human
      Sample Type: Plasma
    39. Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?
      Authors: Naik, Pooja, Fofaria, Neel, Prasad, Shikha, Sajja, Ravi K, Weksler, Babette, Couraud, Pierre-O, Romero, Ignacio, Cucullo, Luca
      BMC Neurosci, 2014;15(0):51.
      Species: Human
      Sample Type: Cell Culture Supernates
    40. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
      Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
      PLoS ONE, 2014;9(6):e99612.
      Species: Human
      Sample Type: Cell Culture Supernates
    41. CREB-induced inflammation is important for malignant mesothelioma growth.
      Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
      Am J Pathol, 2014;184(10):2816-27.
      Species: Human
      Sample Type: Cell Culture Supernates
    42. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
      Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
      Diabetol Metab Syndr, 2014;6(1):28.
      Species: Human
      Sample Type: Plasma
    43. Looking beyond endotoxin: a comparative study of pyrogen retention by ultrafilters used for the preparation of sterile dialyis fluid.
      Authors: Glorieux, Griet, Hulko, Michael, Speidel, Rose, Brodbeck, Katrin, Krause, Bernd, Vanholder, Raymond
      Sci Rep, 2014;4(0):6390.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
      Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
      PLoS ONE, 2014;9(5):e98517.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
      Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
      PLoS Pathog, 2014;10(8):e1004311.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Tissue Homogenates
    46. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
      Authors: Chou C, Lin F, Tsai H, Chang S
      BMC Pulm Med, 2014;14(0):182.
      Species: Human
      Sample Type: BALF
    47. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
      Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
      BMC Biotechnol, 2014;14(0):63.
      Species: Human
      Sample Type: Serum
    48. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
      Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
      J Lipid Res, 2013;54(11):2998-3008.
      Species: Human
      Sample Type: Cell Culture Supernates
    49. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
      Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
      Blood, 2013;121(1):95-106.
      Species: Human
      Sample Type: Cell Culture Supernates
    50. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
      Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
      J Neuroinflammation, 2013;10(0):29.
      Species: Human
      Sample Type: CSF
    51. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
      Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
      Stem Cells, 2013;31(1):146-55.
      Species: Human
      Sample Type: Tissue Homogenates
    52. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
      Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
      PLoS ONE, 2012;7(7):e41383.
      Species: Human
      Sample Type: Cell Culture Supernates
    53. Validation and quality control of ELISAs for the use with human saliva samples.
      Authors: Jaedicke KM, Taylor JJ, Preshaw PM
      J. Immunol. Methods, 2012;377(1):62-5.
      Species: Human
      Sample Type: Saliva
    54. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
      Acta Pharmacol. Sin., 2012;33(9):1204-16.
      Species: Human
      Sample Type: Cell Culture Supernates
    55. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
      Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
      Eur. Respir. J., 2011;38(5):1019-28.
      Species: Human
      Sample Type: Sputum
    56. High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
      Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
      J. Bone Miner. Res., 2011;26(9):2082-95.
      Species: Human
      Sample Type: Cell Culture Supernates
    57. IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease.
      Authors: Pathak S, Goldofsky E, Vivas EX, Bonagura VR, Vambutas A
      J. Immunol., 2011;186(3):1870-9.
      Species: Human
      Sample Type: Plasma
    58. Bacillus anthracis capsule activates caspase-1 and induces interleukin-1beta release from differentiated THP-1 and human monocyte-derived dendritic cells.
      Authors: Cho MH, Ahn HJ, Ha HJ, Park J, Chun JH, Kim BS, Oh HB, Rhie GE
      Infect. Immun., 2010;78(1):387-92.
      Species: Human
      Sample Type: Cell Culture Supernates
    59. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
      Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
      J. Immunol., 2010;185(7):4457-69.
      Species: Human
      Sample Type: Serum
    60. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
      Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
      J. Allergy Clin. Immunol., 2010;126(5):962.
      Species: Human
      Sample Type: Tissue Homogenates
    61. Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
      Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
      J. Immunol., 2009;182(1):547-53.
      Species: Human
      Sample Type: Cell Culture Supernates
    62. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
      Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
      Atherosclerosis, 2009;203(2):503-8.
      Species: Human
      Sample Type: Plasma
    63. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
      Authors: Chase JC, Celli J, Bosio CM
      Infect. Immun., 2009;77(1):180-95.
      Species: Human
      Sample Type: Cell Culture Supernates
    64. Characteristics of dengue virus-infected peripheral blood mononuclear cell death that correlates with the severity of illness.
      Authors: Jaiyen Y, Masrinoul P, Kalayanarooj S, Pulmanausahakul R, Ubol S
      Microbiol. Immunol., 2009;53(8):442-50.
      Species: Human
      Sample Type: Plasma
    65. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
      Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
      J. Exp. Med., 2009;206(3):535-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    66. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
      Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
      Cancer Res., 2009;69(20):8191-9.
    67. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
      Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
      Clin. Sci., 2009;116(10):781-9.
      Species: Human
      Sample Type: Plasma
    68. Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
      Authors: Semiramoth N, Gleizes A, Turbica I, Sandre C, Gorges R, Kansau I, Servin A, Chollet-Martin S
      J. Leukoc. Biol., 2009;85(2):310-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    69. Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome.
      Authors: Duncan JA, Gao X, Huang MT, O'Connor BP, Thomas CE, Willingham SB, Bergstralh DT, Jarvis GA, Sparling PF, Ting JP
      J. Immunol., 2009;182(10):6460-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    70. The role of nerve growth factor in hyperosmolar stress induced apoptosis.
      Authors: Chang EJ, Im YS, Kay EP
      J. Cell. Physiol., 2008;216(1):69-77.
      Species: Human
      Sample Type: Cell Culture Supernates
    71. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
      Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
      J. Interferon Cytokine Res., 2008;28(5):297-310.
      Species: Human
      Sample Type: Cell Culture Supernates
    72. Decreased lubricin concentrations and markers of joint inflammation in the synovial fluid of patients with anterior cruciate ligament injury.
      Authors: Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD
      Arthritis Rheum., 2008;58(6):1707-15.
      Species: Human
      Sample Type: Synovial Fluid
    73. Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
      Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
      Anal. Chem., 2008;80(12):4741-51.
      Species: Human
      Sample Type: Reference Standard
    74. Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
      Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
      Arthritis Rheum., 2008;58(11):3430-5.
      Species: Human
      Sample Type: Synovial Fluid
    75. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
      Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
      FEBS Lett., 2007;581(9):1928-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    76. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
      Authors: Rao DA, Tracey KJ, Pober JS
      J. Immunol., 2007;179(10):6536-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    77. C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
      Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
      Atherosclerosis, 2007;196(1):129-35.
      Species: Human
      Sample Type: Cell Culture Supernates
    78. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
      Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
      Am. J. Respir. Crit. Care Med., 2007;177(7):763-70.
      Species: Human
      Sample Type: Whole Cells
    79. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
      Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
      Fertil. Steril., 2007;90(1):60-4.
      Species: Human
      Sample Type: Follicular Fluid
    80. Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
      Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
      Int. J. Oncol., 2007;31(1):97-102.
      Species: Human
      Sample Type:
    81. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
      Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
      Stem Cells, 2007;26(1):279-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    82. Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
      Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
      Species: Human
      Sample Type: BALF
    83. Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
      Authors: Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
      J. Immunol., 2006;176(9):5284-92.
      Species: Human
      Sample Type: Cell Culture Supernates
    84. The potential of adiponectin in driving arthritis.
      Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
      J. Immunol., 2006;176(7):4468-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    85. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
      Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
      Int. J. Cancer, 2006;119(10):2313-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    86. Dental plaque, gingival inflammation, and elevated levels of interleukin-6 and cortisol in gingival crevicular fluid from women with stress-related depression and exhaustion.
      Authors: Johannsen A, Rylander G, Soder B, Asberg M
      J. Periodontol., 2006;77(8):1403-9.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    87. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
      Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
      Clin. Cancer Res., 2006;12(7):2055-60.
      Species: Human
      Sample Type: Plasma
    88. Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
      Authors: Peterson ML, Schlievert PM
      Biochemistry, 2006;45(7):2387-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    89. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
      Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
      Arch. Intern. Med., 2006;166(3):366-8.
      Species: Human
      Sample Type: Plasma
    90. Kinin receptor expression during Staphylococcus aureus infection.
      Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
      Blood, 2006;108(6):2055-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    91. Non-esterified fatty acid concentrations are independently associated with hepatic steatosis in obese subjects.
      Authors: Holt HB, Wild SH, Wood PJ, Zhang J, Darekar AA, Dewbury K, Poole RB, Holt RI, Phillips DI, Byrne CD
      Diabetologia, 2006;49(1):141-8.
      Species: Human
      Sample Type: Serum
    92. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
      Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
      Cell, 2006;124(6):1225-39.
      Species: Human
      Sample Type: Cell Culture Supernates
    93. The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
      Authors: Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
      Proc. Natl. Acad. Sci. U.S.A., 2006;103(26):9982-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    94. Humoral and cellular factors responsible for coronary collateral formation.
      Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
      Am. J. Cardiol., 2006;98(9):1194-7.
      Species: Human
      Sample Type: Plasma
    95. Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells.
      Authors: Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P
      Cytokine, 2006;36(5):211-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    96. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
      Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
      Blood, 2006;108(3):947-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    97. Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
      Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
      Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(3):276-84.
      Species: Human
      Sample Type: Synovial Fluid
    98. Interleukin-1beta tear concentration in glaucomatous and ocular hypertensive patients treated with preservative-free nonselective beta-blockers.
      Authors: Manni G, Centofanti M, Oddone F, Parravano M, Bucci MG
      Am. J. Ophthalmol., 2005;139(1):72-7.
      Species: Human
      Sample Type: Tears
    99. Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.
      Authors: Bedaiwy MA, Falcone T, Goldberg JM, Attaran M, Sharma R, Miller K, Nelson DR, Agarwal A
      J. Assist. Reprod. Genet., 2005;22(4):161-5.
      Species: Human
      Sample Type: Hypersalpingeal Fluid (HSF)
    100. Aseptic peritonitis due to peptidoglycan contamination of pharmacopoeia standard dialysis solution.
      Authors: Martis L, Patel M, Giertych J, Mongoven J, Taminne M, Perrier MA, Mendoza O, Goud N, Costigan A, Denjoy N, Verger C, Owen WF
      Lancet, 2005;365(9459):588-94.
      Species: Human
      Sample Type: Complex Sample Type
    101. Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition.
      Authors: Witte RP, Kao WJ
      Biomaterials, 2005;26(17):3673-82.
      Species: Human
      Sample Type: Whole Cells
    102. Mucosal wound healing in a rabbit model of subglottic stenosis: biochemical analysis of secretions.
      Authors: Branski RC, Sandulache VC, Dohar JE, Hebda PA
      Arch. Otolaryngol. Head Neck Surg., 2005;131(2):153-7.
      Species: Rabbit
      Sample Type: Tissue Secretion
    103. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
      Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
      J. Orthop. Res., 2005;23(2):397-405.
      Species: Human
      Sample Type: Synovial Fluid
    104. Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
      Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
      J. Allergy Clin. Immunol., 2005;116(4):827-35.
      Species: Human
      Sample Type: Cell Culture Supernates
    105. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
      Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
      J. Periodont. Res., 2004;39(3):158-67.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    106. The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases.
      Authors: Wei PF, Ho KY, Ho YP, Wu YM, Yang YH, Tsai CC
      J. Periodont. Res., 2004;39(5):287-93.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    107. Markers of wound healing in vocal fold secretions from patients with laryngeal pathology.
      Authors: Branski RC, Rosen CA, Verdolini K, Hebda PA
      Ann. Otol. Rhinol. Laryngol., 2004;113(1):23-9.
      Species: Human
      Sample Type: Tissue Secretion
    108. Tissue adhesiveness and host response of in situ photopolymerizable interpenetrating networks containing methylprednisolone acetate.
      Authors: Zilinski JL, Kao WJ
      J Biomed Mater Res A, 2004;68(2):392-400.
      Species: Human
      Sample Type: Tissue Perfusion Fluid
    109. Guiding the vaginal microbicide trials with biomarkers of inflammation.
      Authors: Fichorova RN
      J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
      Species: Human
      Sample Type: Vaginal Lavage
    110. Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury.
      Authors: Hayakata T, Shiozaki T, Tasaki O, Ikegawa H, Inoue Y, Toshiyuki F, Hosotubo H, Kieko F, Yamashita T, Tanaka H, Shimazu T, Sugimoto H
      Shock, 2004;22(2):102-7.
      Species: Human
      Sample Type: Serum
    111. Determinants of plasma leptin concentration in postmenopausal women.
      Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
      Mech. Ageing Dev., 2004;125(2):117-9.
      Species: Human
      Sample Type: Whole Cells
    112. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
      Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
      J. Androl., 2004;25(2):250-4.
      Species: Human
      Sample Type: Seminal Plasma
    113. Shifts in biochemical markers associated with wound healing in laryngeal secretions following phonotrauma: a preliminary study.
      Authors: Verdolini K, Rosen CA, Branski RC, Hebda PA
      Ann. Otol. Rhinol. Laryngol., 2003;112(12):1021-5.
      Species: Human
      Sample Type: Tissue Secretion
    114. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
      Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
      Int. J. Cancer, 2002;101(2):183-9.
      Species: Human
      Sample Type: Ascites Fluid
    115. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
      Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
      J. Allergy Clin. Immunol., 2002;110(4):624-33.
      Species: Human
      Sample Type: Cell Culture Supernates
    116. Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
      Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
      Eur. J. Immunol., 2002;32(5):1464-71.
      Species: Human
      Sample Type: Cell Culture Supernates
    117. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
      Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
      Blood, 2001;97(9):2758-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    118. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
      Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
      J. Immunol., 2001;167(8):4693-700.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    119. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
      Authors: Carstanjen D, Regenfus M, Muller C, Salama A
      Exp. Hematol., 2001;29(7):812-21.
      Species: Human
      Sample Type: Plasma
    120. Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis.
      Authors: Rasmussen L, Hanstrom L, Lerner UH
      J. Clin. Periodontol., 2000;27(1):41-52.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    121. Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins.
      Authors: Attur MG, Dave MN, Clancy RM, Patel IR, Abramson SB, Amin AR
      J. Immunol., 2000;164(5):2684-91.
      Species: Human
      Sample Type: Cell Culture Supernates
    122. Luminal neutrophil migration in ileoanal pouches studied by whole gut lavage.
      Authors: Evgenikos NL, Bartolo DC, Hamer-Hodges DW, Ghosh S
      Eur J Gastroenterol Hepatol, 2000;12(5):553-7.
      Species: Human
      Sample Type: Gut Lavage Fluid
    123. Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.
      Authors: Welty-Wolf KE, Carraway MS, Ghio A, Kantrow SP, Huang YC, Piantadosi CA
      Shock, 2000;13(5):404-9.
      Species: Primate - Papio cynocephalus (Yellow Baboon)
      Sample Type: Serum
    124. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    125. Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
      Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
      Eur. J. Oral Sci., 1998;106(3):778-87.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    126. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
      Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
      J Clin Invest, 1998;101(4):890-8.
      Species: Human
      Sample Type: Cell Culture Supernates
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     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
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      Anonymous 06/20/2017
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     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
    : Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50]

    Summary

    Sample TestedTHP-1 human acute monocytic leukemia cell line
     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
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      Anonymous 04/24/2017
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     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
    : Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50]

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    Sample TestedSerum

    Other Experimental Details

    Other Experimental DetailsThe Human IL-1beta Quantikine ELISA kit is used for diagnostic use.
     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
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      Anonymous 03/15/2017
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     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
    : Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50]

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    Sample TestedPeripheral blood mononuclear cells (PBMCs)

    Other Experimental Details

    Other Experimental DetailsFreshly isolated PBMCs from buffy coats stimulated with LPS (1ug/mL) for 3h together with Simvastatin. Total amount of 3 million cells/well. Volume supernatant used: 100uL. Two donors. Great production of IL1B in supernatant. Increased when statins are present as expected. Experiment used as a positive control
     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
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      Anonymous 04/13/2016
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     Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
    : Human IL-1 beta/IL-1F2 Quantikine ELISA Kit [DLB50]

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    Sample Testeduman plasma

    FAQs

    1. Does the Human IL-1 beta/IL-1F2 Quantikine® ELISA kit (Catalog # DLB50) detect pro or mature IL-1 beta?

      • The standard included in the kit is mature recombinant IL-1 beta (p17). This is also the protein used as the immunogen for the antibodies in the kit.

        For these reasons, the kit has been designed to measure mature IL-1 beta. In theory, since the same amino acids present in mature IL-1 beta are also present in pro IL-1 beta (p31), it may be possible for the assay to detect pro IL-1 beta, either fully or partially. However, recombinant pro IL-1 beta is not available for R&D Systems to perform cross-reactivity testing.

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