Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

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HSLB00D
PHSLB00D
SSLB00D
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Human IL-1 beta/IL-1F2 Standard Curve
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Product Details
Citations (27)
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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary

Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL)
Sensitivity
0.063 pg/mL
Assay Range
0.1 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human IL-1 beta. This assay also recognizes recombinant rhesus macaque IL-1 beta (aa 117-269).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.315 1.3 2.52 0.27 1.36 2.6
Standard Deviation 0.014 0.05 0.06 0.029 0.101 0.163
CV% 4.4 3.9 2.4 10.7 7.4 6.3

Recovery

The recovery of human IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 87-106
Heparin Plasma (n=4) 92 89-99
Serum (n=4) 91 86-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-1 beta/IL-1F2 ELISA Linearity

Data Examples

Human IL-1 beta/IL-1F2 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat
    Authors: M Le Dinh, S Carreira, J Obert, G Gayan-Rami, B Riou, M Beuvin, T Similowski, C Coirault, A Demoule
    PLoS ONE, 2018;13(8):e0200429.
    Species: Human
    Sample Types: Plasma
  2. Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression
    Authors: M Hollborn, C Ackmann, H Kuhrt, F Doktor, L Kohen, P Wiedemann, A Bringmann
    Mol. Vis., 2018;24(0):518-535.
    Species: Human
    Sample Types: Cell Lysates
  3. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2017;4(1):56-65.
    Species: Human
    Sample Types: Serum
  4. Inflammation and Arterial Stiffness in Chronic Kidney Disease: Findings From the CRIC Study
    Authors: E Peyster, J Chen, HI Feldman, AS Go, J Gupta, N Mitra, Q Pan, A Porter, M Rahman, D Raj, M Reilly, MR Wing, W Yang, RR Townsend,
    Am. J. Hypertens., 2017;30(4):400-408.
    Species: Human
    Sample Types: Plasma
  5. Aberrant Inflammasome Activation Characterizes Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome
    Authors: HY Tan, YK Yong, EM Shankar, G Paukovics, R Ellegård, M Larsson, A Kamarulzam, MA French, SM Crowe
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses.
    Authors: Kim H, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, Lee S, Kim Y, Suzuki K, Sakamoto S
    PLoS ONE, 2015;10(9):e0137567.
    Species: Human
    Sample Types: Plasma
  7. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
    Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
    Respir Res, 2014;15(0):114.
    Species: Human
    Sample Types: Sputum
  8. Endothelial activation by platelets from sickle cell anemia patients.
    Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
    PLoS ONE, 2014;9(2):e89012.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans.
    Authors: Zhou X, Yuan F, Ji W, Guo Z, Zhang L, Lu R, Liu X, Liu H, Zhang W, Jiang T, Zhang Z, Li Y
    Obesity (Silver Spring), 2014;22(6):1470-6.
    Species: Human
    Sample Types: Serum
  10. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013;10(0):29.
    Species: Human
    Sample Types: Serum
  11. Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational study.
    Authors: Beghe B, Verduri A, Bottazzi B, Stendardo M, Fucili A, Balduzzi S, Leuzzi C, Papi A, Mantovani A, Fabbri L, Ceconi C, Boschetto P
    PLoS ONE, 2013;8(11):e80166.
    Species: Human
    Sample Types: Plasma
  12. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
    Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
    BMC Pulm Med, 2012;12(0):3.
    Species: Human
    Sample Types: Plasma
  13. Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome.
    Authors: Schneider MP, Schlaich MP, Harazny JM, Raff U, Ritt M, Ott C, Schmieder RE
    Obesity (Silver Spring), 2011;19(5):960-7.
    Species: Human
    Sample Types: Plasma
  14. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
    Authors: Verma S, Kumar M, Nerurkar VR
    J. Gen. Virol., 2011;92(0):507-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
    Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
    PLoS ONE, 2011;6(7):e22748.
    Species: Human
    Sample Types: Plasma
  16. Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
    Authors: Backstrom E, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2010;42(2):149-60.
    Species: Human
    Sample Types: Plasma
  17. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
    Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
    Inflamm. Res., 2010;59(5):339-47.
    Species: Human
    Sample Types: Serum
  18. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2010;210(2):536-41.
    Species: Human
    Sample Types: Plasma
  19. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
    J. Interferon Cytokine Res., 2010;30(11):835-42.
    Species: Human
    Sample Types: Serum
  20. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009;283(1):29-35.
    Species: Human
    Sample Types: Serum
  21. Positive affect and inflammation during radiation treatment for breast and prostate cancer.
    Authors: Sepah SC, Bower JE
    Brain Behav. Immun., 2009;23(8):1068-72.
    Species: Human
    Sample Types: Serum
  22. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
    Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
    J. Exp. Med., 2009;206(5):1029-36.
    Species: Human
    Sample Types: Plasma
  23. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
    Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
    Transplantation, 2009;88(4):582-8.
    Species: Human
    Sample Types: Serum
  24. Correlation between salivary IL-1beta levels and periodontal clinical status.
    Authors: Tobon-Arroyave S, Jaramillo-Gonzalez P, Isaza-Guzman D
    Arch Oral Biol, 2008;53(4):346-52.
    Species: Human
    Sample Types: Saliva
  25. Defining cancer cachexia in head and neck squamous cell carcinoma.
    Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
    Clin. Cancer Res., 2007;13(22):6561-7.
    Species: Human
    Sample Types: Serum
  26. The influence of anger expression on wound healing.
    Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
    Brain Behav. Immun., 2007;22(5):699-708.
    Species: Human
    Sample Types: Tissue Secretion
  27. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Types: Plasma

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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 08/04/2017
Sample Tested: Peripheral blood neutrophils

100uL of sample (neat for my experiments) is required per well. Easy to follow instructions.