Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D: R&D Systems

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

(34 citations)
(1 Review)
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL)
  • Sensitivity
    0.063 pg/mL
  • Assay Range
    0.1 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human IL-1 beta. This assay also recognizes recombinant rhesus macaque IL-1 beta (aa 117-269).
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC41, Quantikine Immunoassay Control Group 10 - Please Inquire
Product Summary
The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0140.050.060.0290.1010.163

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0290.0960.140.0320.1210.29


The recovery of human IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 87-106
Heparin Plasma (n=4) 92 89-99
Serum (n=4) 91 86-101
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
    • Long Name
      Interleukin 1 beta
    • Entrez Gene IDs
      3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig);
    • Alternate Names
      catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    34 Citations: Showing 1 - 10
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    Sample Type
    1. Interleukin-1? levels predict long-term mortality and need for heart transplantation in ambulatory patients affected by idiopathic dilated cardiomyopathy
      Authors: A Aleksova, AP Beltrami, C Carriere, G Barbati, P Lesizza, M Perrieri-M, M Isola, P Gentile, E Salvioni, T Not, P Agostoni, G Sinagra
      Oncotarget, 2017;8(15):25131-25140.
      Species: Human
      Sample Type: Plasma
    2. Aberrant Inflammasome Activation Characterizes Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome
      Authors: HY Tan, YK Yong, EM Shankar, G Paukovics, R Ellegård, M Larsson, A Kamarulzam, MA French, SM Crowe
      J Immunol, 2016;0(0):.
      Species: Human
      Sample Type: Plasma
    3. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses.
      Authors: Kim H, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, Lee S, Kim Y, Suzuki K, Sakamoto S
      PLoS ONE, 2015;10(9):e0137567.
      Species: Human
      Sample Type: Plasma
    4. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
      Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
      PLoS ONE, 2015;10(4):e0124772.
      Species: Human
      Sample Type: Plasma
    5. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
      Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
      Eur J Immunol, 2014;44(5):1552-62.
      Species: Human
      Sample Type: Plasma
    6. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
      Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
      Obesity (Silver Spring), 2014;22(5):1359-66.
      Species: Human
      Sample Type: Plasma
    7. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
      Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
      Respir Res, 2014;15(0):114.
      Species: Human
      Sample Type: Sputum
    8. Endothelial activation by platelets from sickle cell anemia patients.
      Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
      PLoS ONE, 2014;9(2):e89012.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans.
      Authors: Zhou X, Yuan F, Ji W, Guo Z, Zhang L, Lu R, Liu X, Liu H, Zhang W, Jiang T, Zhang Z, Li Y
      Obesity (Silver Spring), 2014;22(6):1470-6.
      Species: Human
      Sample Type: Serum
    10. Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational study.
      Authors: Beghe B, Verduri A, Bottazzi B, Stendardo M, Fucili A, Balduzzi S, Leuzzi C, Papi A, Mantovani A, Fabbri L, Ceconi C, Boschetto P
      PLoS ONE, 2013;8(11):e80166.
      Species: Human
      Sample Type: Plasma
    11. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
      Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
      J Neuroinflammation, 2013;10(0):29.
      Species: Human
      Sample Type: Serum
    12. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
      Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
      BMC Pulm Med, 2012;12(0):3.
      Species: Human
      Sample Type: Plasma
    13. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
      Authors: Verma S, Kumar M, Nerurkar VR
      J. Gen. Virol., 2011;92(0):507-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    15. Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome.
      Authors: Schneider MP, Schlaich MP, Harazny JM, Raff U, Ritt M, Ott C, Schmieder RE
      Obesity (Silver Spring), 2011;19(5):960-7.
      Species: Human
      Sample Type: Plasma
    16. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
      Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
      Inflamm. Res., 2010;59(5):339-47.
      Species: Human
      Sample Type: Serum
    17. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
      Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
      J. Interferon Cytokine Res., 2010;30(11):835-42.
      Species: Human
      Sample Type: Serum
    18. Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
      Authors: Backstrom E, Lappalainen U, Bry K
      Am. J. Respir. Cell Mol. Biol., 2010;42(2):149-60.
      Species: Human
      Sample Type: Plasma
    19. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    20. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
      Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
      Transplantation, 2009;88(4):582-8.
      Species: Human
      Sample Type: Serum
    21. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
      Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
      J. Exp. Med., 2009;206(5):1029-36.
      Species: Human
      Sample Type: Plasma
    22. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
      Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
      J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
      Species: Human
      Sample Type: Plasma
    23. Positive affect and inflammation during radiation treatment for breast and prostate cancer.
      Authors: Sepah SC, Bower JE
      Brain Behav. Immun., 2009;23(8):1068-72.
      Species: Human
      Sample Type: Serum
    24. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
      Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
      Cancer Lett., 2009;283(1):29-35.
      Species: Human
      Sample Type: Serum
    25. Correlation between salivary IL-1beta levels and periodontal clinical status.
      Authors: Tobon-Arroyave S, Jaramillo-Gonzalez P, Isaza-Guzman D
      Arch Oral Biol, 2008;53(4):346-52.
      Species: Human
      Sample Type: Saliva
    26. The influence of anger expression on wound healing.
      Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
      Brain Behav. Immun., 2007;22(5):699-708.
      Species: Human
      Sample Type: Tissue Secretion
    27. Defining cancer cachexia in head and neck squamous cell carcinoma.
      Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
      Clin. Cancer Res., 2007;13(22):6561-7.
      Species: Human
      Sample Type: Serum
    28. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
      Species: Human
      Sample Type: Plasma
    29. Increased interleukin-18 in patients with juvenile idiopathic arthritis and early attachment loss.
      Authors: Miranda LA, Fischer RG, Sztajnbok FR, Johansson A, Figueredo CM, Gustafsson A
      J. Periodontol., 2005;76(1):75-82.
      Species: Human
      Sample Type: Serum
    30. Markers of inflammation in exhaled breath condensate of young healthy smokers.
      Authors: Garey KW, Neuhauser MM, Robbins RA, Danziger LH, Rubinstein I
      Chest, 2004;125(1):22-6.
      Species: Human
      Sample Type: Exhaled Breath Condensate (EBC)
    31. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
      Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
      Immunity, 2003;18(1):87-96.
      Species: Human
      Sample Type: Serum
    32. Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection.
      Authors: Geisbert TW, Hensley LE, Larsen T, Young HA, Reed DS, Geisbert JB, Scott DP, Kagan E, Jahrling PB, Davis KJ
      Am. J. Pathol., 2003;163(6):2347-70.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    33. Impact of an exercise-induced increase in cardiac troponin I in chronic heart failure secondary to ischemic or idiopathic dilated cardiomyopathy.
      Authors: Schulz O, Sigusch HH
      Am. J. Cardiol., 2002;90(5):547-50.
      Species: Human
      Sample Type: Serum
    34. Cytokine production in rhesus monkeys infected with Plasmodium coatneyi.
      Authors: Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE
      Am. J. Trop. Med. Hyg., 1999;61(2):226-9.
      Species: Primate
      Sample Type: Serum
    Expand to show all 34 Citations
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     Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
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     Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
    : Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit [HSLB00D]


    Sample TestedPeripheral blood neutrophils

    Other Experimental Details

    Other Experimental Details100uL of sample (neat for my experiments) is required per well. Easy to follow instructions.

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