Human IL-1 RII APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB663A
Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry.
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Citations (1)
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Human IL-1 RII APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI,  recombinant mouse IL-1ra,  or recombinant rat IL-1ra.
Source
Monoclonal Mouse IgG1 Clone # 34141
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-1 RII antibody in Human PBMCs stimulated to induce Tregs antibody by Flow Cytometry. View Larger

Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs) and gated on CD4+, were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP PE-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL-1 RII APC-conjugated Monoclonal Antibody (Catalog # FAB663A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of IL‑1 RII in HDLM-2 cells by Flow Cytometry HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII APC‑conjugated Monoclonal Antibody (Catalog # FAB663A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor II
Entrez Gene IDs
7850 (Human); 16178 (Mouse)
Alternate Names
beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725

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Citation for Human IL-1 RII APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Authors: C Spano, G Grisendi, G Golinelli, F Rossignoli, M Prapa, M Bestagno, O Candini, T Petrachi, A Recchia, F Miselli, G Rovesti, G Orsi, A Maiorana, P Manni, E Veronesi, MS Piccinno, A Murgia, M Pinelli, EM Horwitz, S Cascinu, P Conte, M Dominici
    Sci Rep, 2019-02-11;9(1):1788.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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