Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 34141
View all IL-1 RII Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930

Specificity

Detects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI,  recombinant mouse IL-1ra,  or recombinant rat IL-1ra.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑1 RII PE‑conjugated Antibody

Detection of IL‑1 RII in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-1 RII antibody in Human PBMCs stimulated to induce Tregs antibody by Flow Cytometry.

Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs), were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P). Cells were gated on CD4. View our protocol for Staining Membrane-associated Proteins.

Detection of IL‑1 RII in HDLM-2 cells by Flow Cytometry

HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII PE‑conjugated Monoclonal Antibody (Catalog # FAB663P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL‑1 RII PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: -  Human peripheral blood mononuclear cells (PBMCs), resting, and stimulated to induce Regulatory T Cells (Tregs), treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Human IL‑2 (Catalog # 202-IL).
- PBMC monocytes
- HDLM-2 cells

Reviewed Applications

Read 1 review rated 5 using FAB663P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor II

Alternate Names

CD121b, IL-1 R beta, IL-1RII, IL1R2, IL1RII

Entrez Gene IDs

7850 (Human); 16178 (Mouse)

Gene Symbol

IL1R2

UniProt

P27930

Additional IL-1 RII Products

Product Documents for Human IL‑1 RII PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑1 RII PE‑conjugated Antibody

For research use only

Citations for Human IL‑1 RII PE‑conjugated Antibody

Customer Reviews for Human IL‑1 RII PE‑conjugated Antibody (1)

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  • Human IL-1 RII PE-conjugated Antibody
    Name: Vincent Giacalone
    Application: Flow Cytometry
    Sample Tested: peripheral blood eosinophils
    Species: Human
    Verified Customer | Posted 07/23/2020
    This antibody was used to stain human peripheral blood for analysis by flow cytometry. Eosinophils were identified from CD45+ leukocytes and IL-1RII expression was measured in the PE channel. This antibody generates consistent fluorescence which is maintained after fixation. Can be used for various cell types.
    Human IL‑1 RII PE‑conjugated Antibody FAB663P

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail