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Human IL-10 Fluorescein-conjugated Antibody
- Species ReactivityHuman
- SpecificityDetects human IL-10 in ELISAs. In sandwich immunoassays, less than 5% cross-reactivity with recombinant equine IL-10 is observed and less than 0.5% cross-reactivity with recombinant canine IL-10, recombinant mouse IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant cotton rat IL-10, and recombinant feline IL-10 is observed.
- SourceMonoclonal Mouse IgG1 Clone # 127107
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelFluorescein
Applications
-
Recommended
ConcentrationSample
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Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
|
Intracellular Staining by Flow Cytometry
|
Detection of IL‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL‑10 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2172F) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-10
Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
- Long Name:Interleukin 10
- Entrez Gene IDs:3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
- Alternate Names:CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
- B Cell Development and Differentiation
- B Cell Markers
- Conventional/Classical Dendritic Cells
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Angiogenesis
- Cytokines and Receptors in Tfh Cell Differentiation and Function
- gamma delta T Cells
- IL-10/Interferon Family
- Inflammatory Mediators
- Isolation, Culture, and Identification of Natural Killer Cells
- Lymphoma
- Macrophage Activation Markers
- Monocyte Markers
- Multiple Sclerosis and Myelin Related Molecules
- Myeloid-derived Suppressor Cells (MDSC)
- Natural Killer T (NKT) Cells
- Neuroinflammation
- Plasmacytoid Dendritic Cells
- Regulatory T Cells (Tregs)
- T Cell Cytokine Signaling
- Th1 Cells
- Th2 Cells
- Th22 Cells
- Th9 Cells
Citations:
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Mouse IgG1 Fluorescein-conjugated Antibody
Ctrl IC002F 9View Sizes & Prices
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Staining Reagents
Description
Application
Cat#
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Flow Cytometry Permeabilization/Wash Buffer I (1X)
Flow FC005 4View Sizes & Prices
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Flow Cytometry Fixation Buffer (1X)
Flow FC004 3View Sizes & Prices
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Flow Cytometry Fixation & Permeabilization Buffer Kit I
Flow FC009View Sizes & Prices
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)
Flow FC007View Sizes & Prices
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