Human IL-15 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 34559
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL-15
    Accession # P40933
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑15 in LPS-treated Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes, resting (open histogram), or treated overnight with 1 μg/mL LPS (filled histogram), were stained with Mouse Anti-Human IL‑15 APC‑conjugated Monoclonal Antibody (Catalog # IC2471A) or isotype control antibody (Catalog # IC002A, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
  • References:
    1. Grabstein, K. et al. (1994) Science 264:965.
    2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
    3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    5. Giri, J.G. et al. (1995) EMBO 14:3654.
    6. Giri, J. et al. (1994) EMBO J. 13:2822.
    7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
    8. Dubois, S. et al. (2002) Immunity 17:537.
    9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
    10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
    14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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