Human IL-15 Antibody

Catalog # Availability Size / Price Qty
MAB2471-SP
MAB2471-100
MAB2471-500
Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)

Human IL-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
Source
Monoclonal Mouse IgG1 Clone # 34559
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑15 (Catalog # 247-IL)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB2471). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References
  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Citations for Human IL-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15
    Authors: Y Rodríguez-, LG Batista-Ro, A Llopiz-Arz, P Puente-Pér, R Martínez-C, J Castro-Vel, A Santos-Sav
    BMC immunology, 2021-12-18;22(1):79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. CX3CL1 and IL-15 Promote CD8 T cell chemoattraction in HIV and in atherosclerosis
    Authors: S Panigrahi, B Chen, M Fang, D Potashniko, AA Komissarov, A Lebedeva, GM Michaelson, JM Wyrick, SR Morris, SF Sieg, M Paiardini, FJ Villinger, K Harth, VS Kashyap, MJ Cameron, CM Cameron, E Vasilieva, L Margolis, SA Younes, NT Funderburg, DA Zidar, MM Lederman, ML Freeman
    PLoS Pathog, 2020-09-25;16(9):e1008885.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Directed Differentiation of Mobilized Hematopoietic Stem and Progenitor Cells into Functional NK cells with Enhanced Antitumor Activity
    Authors: P Oberoi, K Kamenjarin, JFV Ossa, B Uherek, H Bönig, WS Wels
    Cells, 2020-03-27;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis
    Authors: H Touil, A Kobert, N Lebeurrier, A Rieger, P Saikali, C Lambert, L Fawaz, CS Moore, A Prat, J Gommerman, JP Antel, Y Itoyama, I Nakashima, A Bar-Or
    J Neuroinflammation, 2018-04-19;15(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling
    Authors: YE Du, G Tu, G Yang, G Li, D Yang, L Lang, L Xi, K Sun, Y Chen, K Shu, H Liao, M Liu, Y Hou
    Theranostics, 2017-09-15;7(16):3972-3988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use
    Authors: JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion
    J Immunol Res, 2017-07-13;2017(0):1975902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A Refractory Celiac Patient Successfully Treated With Mesenchymal Stem Cell Infusions
    Authors: R Ciccociopp, A Gallia, MA Avanzini, E Betti, C Picone, A Vanoli, C Paganini, F Biagi, R Maccario, GR Corazza
    Mayo Clin Proc, 2016-04-14;0(0):.
    Species: Human
    Sample Types: BALF
    Applications: Western Blot
  8. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
    Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
    PLoS ONE, 2015-05-07;10(5):e0123340.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus.
    Authors: Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S
    J Immunol, 2015-03-02;194(8):3890-900.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers.
    Authors: Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A
    Cancer Res, 2014-06-30;74(17):4908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  11. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
    Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
    Blood, 2010-04-14;115(25):5170-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Juxtacrine function of interleukin-15/interleukin-15 receptor system in tumour derived human B-cell lines.
    Authors: Tsukamoto K, Huang YC, Dorsey WC, Carns B, Sharma V
    Clin. Exp. Immunol., 2006-12-01;146(3):559-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002-01-01;168(1):505-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. What concentration of LPS was used to stimulate PBMCs prior to staining with Human IL-15 Antibody, Catalog #s MAB2471 and IC2471?

    • PBMCs were stimulated overnight with 1 μg/mL LPS prior to staining with Human IL-15 Antibody. 3mM Monesin was added for the last 3-4 hours of stimulation.

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Human IL-15 Antibody
By Anonymous on 09/30/2021
Application: WB Sample Tested: NK cells Species: Human

Human IL‑15 Antibody
By Anonymous on 02/08/2021
Application: ELISA Sample Tested: Recombinant protein Species: Human