Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry, Blocking, Cell Culture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 34559
View all IL-15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933

Specificity

Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑15 Antibody

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.

Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB2471). The ND50 is typically 0.1-0.5 µg/mL.

Detection of IL‑15 in Human peripheral blood mononuclear cells (PBMCs) treated with 1µg/mL LPS for 16 hrs by Flow Cytometry

Human peripheral blood mononuclear cells (PBMCs) treated with 1µg/mL LPS for 16 hrs were stained with Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB2471, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL‑15 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: See below

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑15 (Catalog # 247-IL)

Neutralization

Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑15.

Reviewed Applications

Read 3 reviews rated 5 using MAB2471 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References

  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

Long Name

Interleukin 15

Alternate Names

IL15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Gene Symbol

IL15

UniProt

P40933

Additional IL-15 Products

Product Documents for Human IL‑15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL‑15 Antibody

For research use only

Citations for Human IL‑15 Antibody

Customer Reviews for Human IL‑15 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human IL‑15 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-15 Antibody
    Name: Anonymous
    Application: I use the GBM cells lines which overexpression with mIL15, and test by the MAB247 IL15 antibody, the immunostaining results shows strong signal of IL15 located on the cell membrane, I am excited about that.
    Sample Tested: A172 human glioblastoma cell line
    Species: Human
    Verified Customer | Posted 05/01/2024
    Human IL‑15 Antibody MAB2471
  • Human IL-15 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: NK cells
    Species: Human
    Verified Customer | Posted 09/30/2021
    Human IL‑15 Antibody MAB2471
  • Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 02/08/2021
    Human IL‑15 Antibody MAB2471

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

FAQs for Human IL‑15 Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q: What concentration of LPS was used to stimulate PBMCs prior to staining with Human IL-15 Antibody, Catalog #s MAB2471 and IC2471?

    A: PBMCs were stimulated overnight with 1 μg/mL LPS prior to staining with Human IL-15 Antibody. 3mM Monesin was added for the last 3-4 hours of stimulation.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies