Human IL-15 Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 34559
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL-15
    Asn49-Ser162
    Accession # P40933
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑15 (Catalog # 247-IL)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
  • Neutralization
    Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑15.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB2471). The ND50 is typically 0.1-0.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
  • References:
    1. Grabstein, K. et al. (1994) Science 264:965.
    2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
    3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    5. Giri, J.G. et al. (1995) EMBO 14:3654.
    6. Giri, J. et al. (1994) EMBO J. 13:2822.
    7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
    8. Dubois, S. et al. (2002) Immunity 17:537.
    9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
    10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
    14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use
    Authors: JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion
    J Immunol Res, 2017;2017(0):1975902.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. A Refractory Celiac Patient Successfully Treated With Mesenchymal Stem Cell Infusions
    Authors: R Ciccociopp, A Gallia, MA Avanzini, E Betti, C Picone, A Vanoli, C Paganini, F Biagi, R Maccario, GR Corazza
    Mayo Clin Proc, 2016;0(0):.
    Species: Human
    Sample Type: BALF
    Application: WB
  3. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
    Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
    PLoS ONE, 2015;10(5):e0123340.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus.
    Authors: Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S
    J Immunol, 2015;194(8):3890-900.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers.
    Authors: Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A
    Cancer Res, 2014;74(17):4908-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  6. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
    Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
    Blood, 2010;115(25):5170-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Juxtacrine function of interleukin-15/interleukin-15 receptor system in tumour derived human B-cell lines.
    Authors: Tsukamoto K, Huang YC, Dorsey WC, Carns B, Sharma V
    Clin. Exp. Immunol., 2006;146(3):559-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002;168(1):505-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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