Human IL-17 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY317
DY317-05
Ancillary Products Available
Human IL-17 / IL-17A ELISA Standard Curve
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Product Details
Procedure
Citations (37)
FAQs
Supplemental Products
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Human IL-17 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-17. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

  

Data Example

Human IL-17 / IL-17A ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Alternate Names:
CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human IL-17 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Berberine exerts an anti-inflammatory role in ocular Behcet's disease
    Mol Med Rep, 2017;15(1):97-102.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017;12(4):e0175109.
    Species: Human
    Sample Types: Serum
  3. TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
    Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
    Clin. Vaccine Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE)
    Authors: V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso
    Arthritis Res. Ther., 2017;19(1):139.
    Species: Human
    Sample Types: Serum
  5. Angiogenic T cell expansion correlates with severity of peripheral vascular damage in systemic sclerosis
    Authors: M Manetti, S Pratesi, E Romano, S Bellando-R, I Rosa, S Guiducci, BS Fioretto, L Ibba-Manne, E Maggi, M Matucci-Ce
    PLoS ONE, 2017;12(8):e0183102.
    Species: Human
    Sample Types: Serum
  6. The heterogeneity of systemic inflammation in bronchiectasis
    Authors: AD Saleh, JD Chalmers, A De Soyza, TC Fardon, SO Koustas, J Scott, AJ Simpson, JS Brown, JR Hurst
    Respir Med, 2017;127(0):33-39.
    Species: Human
    Sample Types: Serum
  7. Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion
    Authors: Mélissa Noack
    Arthritis Res Ther, 2016;18(0):148.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016;6(0):26662.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
    Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Interleukin-23 Receptor Gene Polymorphism May Enhance Expression of the IL-23 Receptor, IL-17, TNF-alpha and IL-6 in Behcet's Disease.
    Authors: Jiang Z, Hennein L, Tao Y, Tao L
    PLoS ONE, 2015;10(7):e0134632.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Cooperation between IL-7 Receptor and Integrin alpha2beta1 (CD49b) Drives Th17-Mediated Bone Loss.
    Authors: El Azreq M, Arseneault C, Boisvert M, Page N, Allaeys I, Poubelle P, Tessier P, Aoudjit F
    J Immunol, 2015;195(9):4198-209.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Cytokine profiles at admission can be related to outcome in AIDS patients with cryptococcal meningitis.
    Authors: Mora D, Fortunato L, Andrade-Silva L, Ferreira-Paim K, Rocha I, Vasconcelos R, Silva-Teixeira D, Nascentes G, Silva-Vergara M
    PLoS ONE, 2015;10(3):e0120297.
    Species: Human
    Sample Types: Serum
  13. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014;9(5):e96943.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. HIF-1alpha- and hypoxia-dependent immune responses in human CD4+CD25high T cells and T helper 17 cells.
    Authors: Bollinger T, Gies S, Naujoks J, Feldhoff L, Bollinger A, Solbach W, Rupp J
    J Leukoc Biol, 2014;96(2):305-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. IFN- alpha blocks IL-17 production by peripheral blood mononuclear cells in patients with chronic active hepatitis B Infection.
    Authors: Cui F, Meng J, Luo P, Chen P
    BMC Infect Dis, 2014;14(0):55.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014;165(1):134-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014;5(0):3199.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels.
    Authors: Arndts, Kathrin, Specht, Sabine, Debrah, Alexande, Tamarozzi, Francesc, Klarmann Schulz, Ute, Mand, Sabine, Batsa, Linda, Kwarteng, Alexande, Taylor, Mark, Adjei, Ohene, Martin, Coralie, Layland, Laura E, Hoerauf, Achim
    PLoS Negl Trop Dis, 2014;8(2):e2679.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. The role of interleukin-1 receptor-associated kinases in Vogt-Koyanagi-Harada disease.
    Authors: Sun M, Yang P, DU L, Yang Y, Ye J
    PLoS ONE, 2014;9(4):e93214.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A
    J Immunol, 2013;190(7):3225-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(8):3001-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. CCR2+CCR5+ T Cells Produce Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis.
    Authors: Sato W, Tomita A, Ichikawa D, Lin Y, Kishida H, Miyake S, Ogawa M, Okamoto T, Murata M, Kuroiwa Y, Aranami T, Yamamura T
    J Immunol, 2012;189(10):5057-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
    Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2011;52(10):7084-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease.
    Authors: Liu X, Yang P, Wang C, Li F, Kijlstra A
    Rheumatology (Oxford), 2011;50(2):293-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.
    Authors: Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y
    Mol. Vis., 2011;17(0):1425-30.
    Species: Human
    Sample Types: Serum
  28. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2011;25(1):409-19.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. IL-27 mediates the response to IFN-beta therapy in multiple sclerosis patients by inhibiting Th17 cells.
    Authors: Sweeney CM, Lonergan R, Basdeo SA, Kinsella K, Dungan LS, Higgins SC, Kelly PJ, Costelloe L, Tubridy N, Mills KH, Fletcher JM
    Brain Behav. Immun., 2010;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  30. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
    Authors: Yu RY, Gallagher G
    J. Immunol., 2010;185(12):7302-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  32. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Characterization of the interleukin-17 isoforms and receptors in lesional psoriatic skin.
    Authors: Johansen C, Usher PA, Kjellerup RB
    Br. J. Dermatol., 2009;160(2):319-24.
    Species: Human
    Sample Types: Tissue Homogenates
  34. Diminished frequency and function of CD4+CD25high regulatory T cells associated with active uveitis in Vogt-Koyanagi-Harada syndrome.
    Authors: Chen L, Yang P, Zhou H, He H, Ren X, Chi W, Wang L, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008;49(8):3475-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Cutting edge: Human Th17 cells are identified as bearing CCR2+CCR5- phenotype.
    Authors: Sato W, Aranami T, Yamamura T
    J. Immunol., 2007;178(12):7525-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
    Authors: Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM
    Cytokine, 2006;35(5):235-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004;173(2):1463-76.
    Species: Human
    Sample Types: Cell Culture Supernates

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