Human IL-1 alpha /IL-1F1 Antibody

Catalog # Availability Size / Price Qty
AF-200-NA
AF-200-SP
Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody.
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 alpha /IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) IL-1 alpha and recombinant rat IL‑1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Ser113-Ala271
Accession # Q53QF9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.75-4.5 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody.

Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat  Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND50 is typically 0.75-4.5 ng/mL.

Immunocytochemistry IL‑1 alpha /IL‑1F1 in Human PBMCs. View Larger

IL‑1 alpha /IL‑1F1 in Human PBMCs.

IL‑1 alpha /IL‑1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

Product Datasheets

Citations for Human IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
    PLoS ONE, 2016;11(9):e0162908.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007;151(1):115-27.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: WB
  4. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
    Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
    Cytokine, 2005;31(2):153-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
    Authors: Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF
    Circ. Res., 2003;93(6):515-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IL-1 alpha /IL-1F1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human IL-1 alpha /IL-1F1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-1 alpha /IL-1F1 Antibody
By Anonymous on 04/02/2018
Application: ICC/IF Sample Tested: Pancreatic cancer cells,Pancreatic cancer tissue Species: Human