Human IL-1 alpha /IL-1F1 Antibody

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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody.
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Human IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Detects human IL-1 alpha /IL-1F1 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Accession # Q53QF9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA)
5-15 µg/mL
See below
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.75-4.5 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody. Recombinant Human IL-1a/IL-1F1 (200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND50 is typically 0.75-4.5 ng/mL.

Immunocytochemistry IL-1a/IL-1F1 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 alpha /IL‑1F1 in Human PBMCs. IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Functional Detection of Human IL-1 alpha/IL-1F1 by Functional View Larger

Detection of Human IL-1 alpha/IL-1F1 by Functional TPM1 and TPM4 expression in response to IL-1 alpha.High molecular weight tropomyosin isoform expression in THP1 cells in response to elevated concentrations of IL-1 alpha. Image collected and cropped by CiteAb from the following open publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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Citations for Human IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. The Pseudomonas aeruginosa protease LasB directly activates IL-1&beta
    Authors: J Sun, DL LaRock, EA Skowronski, JM Kimmey, J Olson, Z Jiang, AJ O'Donoghue, V Nizet, CN LaRock
    EBioMedicine, 2020-09-23;60(0):102984.
  2. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer
    Authors: Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al.
    Cell Death & Disease
  3. Discovery of senolytics using machine learning
    Authors: Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Human TH17 cells engage gasdermin E pores to release IL-1alpha on NLRP3 inflammasome activation
    Authors: YY Chao, A Puhach, D Frieser, M Arunkumar, L Lehner, T Seeholzer, A Garcia-Lop, M van der Wa, S Fibi-Smeta, A Dietschman, T Sommermann, T ?ikovi?, L Taher, MS Gresnigt, SJ Vastert, F van Wijk, G Panagiotou, D Krappmann, O Gro beta, CE Zielinski
    Nature Immunology, 2023-01-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation
    Authors: Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al.
  6. Wu-Zi-Yan-Zong-Wan protects mouse blood-testis barrier from Tripterygium wilfordii Hook. f. multiglycoside-induced disruption by regulating proinflammatory cytokines
    Authors: Z Pan, Y Gao, S Liu, Z Ke, J Guo, W Ma, T Cui, B Liu, X Zhang
    Journal of ethnopharmacology, 2021-07-19;280(0):114440.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  7. Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
    Authors: D Liu, A Steins, R Klaassen, AP van der Za, RJ Bennink, G van Tienho, MG Besselink, MF Bijlsma, HWM van Laarho
    Biomedicines, 2020-10-22;8(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Inhibition
  8. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017-01-01;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
    PLoS ONE, 2016-09-20;11(9):e0162908.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Tissue and serum immune response in chronic hepatitis C with mild histological lesions
    Authors: A T R-Viso, M I S Duarte, C Pagliari, E R Fernandes, R A Brasil, G Benard et al.
    Memórias do Instituto Oswaldo Cruz
  11. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007-03-12;151(1):115-27.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  12. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006-03-24;124(6):1225-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
    Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
    Cytokine, 2005-07-21;31(2):153-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003-12-21;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
    Authors: Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF
    Circ. Res., 2003-08-28;93(6):515-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Human IL-1 alpha /IL-1F1 Antibody
By Anonymous on 04/02/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreatic cancer cells,Pancreatic cancer tissue Species: Human