Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-21R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R Subunit
Cys20-Pro236
Accession # Q9HBE5

Specificity

Detects human IL-21 R Subunit in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse IL‑21 R Subunit is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human IL‑21R Subunit Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood CD19+ B cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)

Reviewed Applications

Read 1 review rated 5 using BAF991 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma  chain ( gamma c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21 Receptor

Alternate Names

CD360, IL-21 R, IL21R, NILR

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Gene Symbol

IL21R

UniProt

Q9HBE5

Additional IL-21R Products

Product Documents for Human IL‑21R Subunit Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑21R Subunit Biotinylated Antibody

For research use only

Citations for Human IL‑21R Subunit Biotinylated Antibody

Customer Reviews for Human IL‑21R Subunit Biotinylated Antibody (1)

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  • Human IL‑21R Subunit Biotinylated Antibody
    Name: Kurt Shanebeck
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/17/2019
    Human IL‑21R Subunit Biotinylated Antibody BAF991

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