Human IL-21R Antibody

Catalog # Availability Size / Price Qty
AF991
AF991-SP
Product Details
Citations (6)
FAQs
Supplemental Products
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Human IL-21R Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑21 R Subunit in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse IL‑21 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑21 R Subunit
Cys20-Pro236
Accession # Q9HBE5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)
Flow Cytometry
2.5 µg/106 cells
Human whole blood CD19+ B cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha  and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells and cell lines of T, B and NK lineage. The common gamma  chain ( gamma c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

References
  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21 Receptor
Entrez Gene IDs
50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names
CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

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Citations for Human IL-21R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
    Authors: Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe J, Harter G, Gruner B, Fabricius D, Lotfi R, Schwarz K, Schutz C, Honig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdorfer B
    J Immunol, 2015-03-16;194(8):3768-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells.
    Authors: de Totero D, Capaia M, Fabbi M, Croce M, Meazza R, Cutrona G, Zupo S, Loiacono F, Truini M, Ferrarini M, Ferrini S
    Exp. Hematol., 2010-03-01;38(5):373-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21.
    Authors: Ansen S, Butler MO, Berezovskaya A, Murray AP, Stevenson K, Nadler LM, Hirano N
    Clin. Cancer Res., 2008-10-01;14(19):6125-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interleukin-21: an interleukin-2 dependent player in rejection processes.
    Authors: Baan CC, Balk AH, Dijke IE, Korevaar SS, Peeters AM, de Kuiper RP, Klepper M, Zondervan PE, Maat LA, Weimar W
    Transplantation, 2007-06-15;83(11):1485-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007-05-01;178(9):5957-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Expression of interleukin-21 receptor, but not interleukin-21, in synovial fibroblasts and synovial macrophages of patients with rheumatoid arthritis.
    Authors: Jungel A, Distler JH, Kurowska-Stolarska M, Seemayer CA, Seibl R, Forster A, Michel BA, Gay RE, Emmrich F, Gay S, Distler O
    Arthritis Rheum., 2004-05-01;50(5):1468-76.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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