Human IL-24 Biotinylated Antibody

Catalog #: BAF1965
3 Citations Datasheet / COA / SDS

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BAF1965

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Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-24 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-24 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL‑24 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑24 (R&D Systems, Catalog # 1965-IL)
Gln50-Leu206
Accession # Q2YHE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑24 (Catalog # 1965-IL)

Human IL-24 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human IL‑24 Antibody (Catalog # MAB19652)

Standard: Recombinant Human IL-24 Protein (Catalog # 1965-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-24

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4⁺ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.

References

Jiang, H. et al. (1995) Oncogene 11:2477.
Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
Wang, M. et al. (2002) J. Biol Chem. 227:7341.
Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Chen, J. et al. (2003) Molec. Ther. 8:220.

Long Name

Interleukin 24

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Alternate Names

C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-24 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Induction of phenotype modifying cytokines by FERMT1 mutations.
Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
Hum. Mutat., 2011-03-01;32(4):397-406.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
Blood, 2009-11-24;115(9):1718-26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24.
Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T
Mol. Ther., 2007-06-05;15(10):1805-11.
Species: Human
Sample Types: Plasma
Applications: ELISA Development