Human IL-24 Antibody Summary
Gln50-Leu206
Accession # Q2YHE5
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody. Recombinant Human IL-24 (Catalog # 1965-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-24 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965). The ND50 is typically < 0.6 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-24
Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.
- Jiang, H. et al. (1995) Oncogene 11:2477.
- Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
- Wang, M. et al. (2002) J. Biol Chem. 227:7341.
- Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Chen, J. et al. (2003) Molec. Ther. 8:220.
Product Datasheets
Citations for Human IL-24 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment
Authors: SC Chang, BX Zhang, EC Su, WC Wu, TH Hsieh, AM Salazar, YK Lin, JL Ding
International Journal of Molecular Sciences, 2021;22(4):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Effects of melanoma differentiation associated gene-7 (MDA-7/IL-24) on apoptosis of liver cancer cells via regulating the expression of B-cell lymphoma-2
Authors: Q Mo, L Liu, G Bao, T Li
Oncol Lett, 2019;18(1):29-34.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Effects of melanoma differentiation associated gene-7 (MDA-7/IL-24) on apoptosis of liver cancer cells via regulating the expression of B-cell lymphoma-2
Authors: Q Mo, L Liu, G Bao, T Li
Oncol Lett, 2019;18(1):29-34.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced tumor growth inhibition by mesenchymal stem cells derived from iPSCs with targeted integration of interleukin24 into rDNA loci
Authors: B Liu, F Chen, Y Wu, X Wang, M Feng, Z Li, M Zhou, Y Wang, L Wu, X Liu, D Liang
Oncotarget, 2017;8(25):40791-40803.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
T cell-derived microvesicles induce mast cell production of IL-24: relevance to inflammatory skin diseases.
Authors: Shefler I, Pasmanik-Chor M, Kidron D, Mekori Y, Hershko A
J Allergy Clin Immunol, 2014;133(1):217-24.e1-3.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expansion of parasite-specific CD4+ and CD8+ T cells expressing IL-10 superfamily cytokine members and their regulation in human lymphatic filariasis.
Authors: Anuradha R, George P, Hanna L, Kumaran P, Chandrasekaran V, Nutman T, Babu S
PLoS Negl Trop Dis, 2014;8(4):e2762.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction of phenotype modifying cytokines by FERMT1 mutations.
Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
Hum. Mutat., 2011;32(4):397-406.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
Blood, 2010;115(9):1718-26.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24.
Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T
Mol. Ther., 2007;15(10):1805-11.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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