Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-24 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑24
Gln50-Leu206
Accession # Q2YHE5

Specificity

Detects human IL-24 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑24 Antibody

Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody.

Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody.

Recombinant Human IL-24 (Catalog # 1965-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-24 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965). The ND50 is typically < 0.6 µg/mL.

Applications for Human IL‑24 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑24 (Catalog # 1965-IL)

Neutralization

Measured by its ability to neutralize IL‑24-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically < 0.6 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑24.

Reviewed Applications

Read 1 review rated 5 using AF1965 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-24

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.

References

  1. Jiang, H. et al. (1995) Oncogene 11:2477.
  2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
  3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  6. Chen, J. et al. (2003) Molec. Ther. 8:220.

Long Name

Interleukin 24

Alternate Names

C49A, FISP, IL24, MDA-7, MOB-5, ST16

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Gene Symbol

IL24

UniProt

Q2YHE5

Additional IL-24 Products

Product Documents for Human IL‑24 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑24 Antibody

For research use only

Citations for Human IL‑24 Antibody

Customer Reviews for Human IL‑24 Antibody (1)

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  • Human IL-24 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 11/04/2016
    Human IL‑24 Antibody AF1965

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