Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 283161
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑24
Gln50-Leu206
Accession # Q13007
Gln50-Leu206
Accession # Q13007
Specificity
Detects human IL-24 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human IL-24 Antibody (283161)
Human IL-24 ELISA Standard Curve
Recombinant Human IL‑24 (Catalog # 1965-IL) was serially diluted and captured by Mouse Anti-Human IL‑24 Monoclonal Antibody (Catalog # MAB19652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human IL-24 Antibody (283161)
Human IL-24 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using MAB19652 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-24
References
- Jiang, H. et al. (1995) Oncogene 11:2477.
- Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
- Wang, M. et al. (2002) J. Biol Chem. 227:7341.
- Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Chen, J. et al. (2003) Molec. Ther. 8:220.
Long Name
Interleukin 24
Alternate Names
C49A, FISP, IL24, MDA-7, MOB-5, ST16
Gene Symbol
IL24
UniProt
Additional IL-24 Products
Product Documents for Human IL-24 Antibody (283161)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-24 Antibody (283161)
For research use only
Related Research Areas
Citations for Human IL-24 Antibody (283161)
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Customer Reviews for Human IL-24 Antibody (283161) (3)
4.3 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 02/07/2020Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/07/2018
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