Human IL-32 Antibody (373821)

R&D Systems | Catalog # MAB30402

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 373821
View all IL-32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-32 alpha
Met1-Asn131
Accession # P24001

Specificity

Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human IL-32 Antibody (373821)

Detection of IL-32 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑32 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 Monoclonal Antibody (Catalog # MAB30402, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL-32 Antibody (373821)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin,  fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-32

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or delta at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).

References

  1. Netea, M.G. et al. (2006) PloS Med. 3:e277.
  2. Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
  3. Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
  4. Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
  5. Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
  6. Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
  7. Hong, J. et al. (2010) Cytokine 49:171.
  8. Kim, S-H. et al. (2005) Immunity 22:131.
  9. Dahl, C.A. et al. (1992) J. Immunol. 148:597.
  10. Choi, J-D. et al. (2009) Immunology 126:535.
  11. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
  12. Kim, S. et al. (2008) BMB Rep. 41:814.
  13. Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
  14. Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
  15. Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
  16. Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
  17. Nold, M.F. et al. (2008) J. Immunol. 181:557.

Long Name

Interleukin 32

Alternate Names

IL32, NK4, TAIF

Entrez Gene IDs

9235 (Human)

Gene Symbol

IL32

UniProt

P24001

Additional IL-32 Products

Product Documents for Human IL-32 Antibody (373821)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-32 Antibody (373821)

For research use only

Citations for Human IL-32 Antibody (373821)

Customer Reviews for Human IL-32 Antibody (373821)

There are currently no reviews for this product. Be the first to review Human IL-32 Antibody (373821) and earn rewards!

Have you used Human IL-32 Antibody (373821)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies