• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    6.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL)
  • Sensitivity
    0.22 pg/mL
  • Assay Range
    0.3 - 16 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant human IL-4
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
Product Summary
The Quantikine HS Human IL-4 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.050.30.940.060.290.9


The recovery of IL-4 was determined by spiking to levels throughout the range of the assay in various matrices.

Sample Type Average % Recovery Range %
Cell Culture Media (n=9) 106 87-118
Serum (n=4) 102 95-108
To assess linearity of the assay, the following samples, spiked with high concentrations of IL-4, were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-4 Quantikine HS ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
    • Long Name
      Interleukin 4
    • Entrez Gene IDs
      3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit);
    • Alternate Names
      B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 50 µL Substrate Solution
    12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.

    13. 50 µL Amplifier Solution
    14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 21
    Filter your results:

    Sample Type
    1. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men.
      Authors: Weng T, Huang S, Chuang Y, Wang J
      PLoS ONE, 2013;8(11):e80248.
      Species: Human
      Sample Type: Plasma
    2. Subnormal cytokine profile in the tear fluid of keratoconus patients.
      Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
      PLoS ONE, 2011;6(1):e16437.
      Species: Human
      Sample Type: Tears
    3. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
      Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
      J. Immunol., 2011;186(9):5254-60.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
      Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
      Mol. Cell Proteomics, 2010;9(2):327-35.
      Species: Human
      Sample Type: Plasma
    5. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    6. Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
      Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
      Proteomics, 2008;8(11):2211-9.
      Species: Human
      Sample Type: Serum
    7. Dysregulated monocyte iron homeostasis and erythropoietin formation in patients with anemia of chronic disease.
      Authors: Theurl I, Mattle V, Seifert M, Mariani M, Marth C, Weiss G
      Blood, 2006;107(10):4142-8.
      Species: Human
      Sample Type: Serum
    8. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
      Species: Human
      Sample Type: Plasma
    9. Anti-inflammatory cytokines in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis: a preliminary report.
      Authors: Bozkurt FY, Yetkin Ay Z, Berker E, Tepe E, Akkus S
      Cytokine, 2006;35(3):180-5.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    10. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
      Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
      Transpl. Int., 2005;18(2):177-85.
      Species: Human
      Sample Type: Plasma
    11. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
      Authors: Jensen J, Krakauer M, Sellebjerg F
      Cytokine, 2005;29(1):24-30.
      Species: Human
      Sample Type: Plasma
    12. Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
      Authors: Bartosik-Psujek H, Stelmasiak Z
      J Neural Transm, 2005;112(6):797-803.
      Species: Human
      Sample Type: Serum
    13. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    14. Antifungal immune reactivity in nasal polyposis.
      Authors: Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L
      Infect. Immun., 2004;72(12):7275-81.
      Species: Human
      Sample Type: nasal lavage
    15. Immunodetection of a hepatitis C virus (HCV) antigen and Thl/Th2 cytokines in cerebrospinal fluid of meningitis patients.
      Authors: Attallah AM, Ibrahim GG
      J Immunoassay Immunochem, 2004;25(4):313-20.
      Species: Human
      Sample Type: Serum
    16. Exhaled breath condensate in children: pearls and pitfalls.
      Authors: Rosias PP, Dompeling E, Hendriks HJ, Heijnens JW, Donckerwolcke RA, Jobsis Q
      Pediatr Allergy Immunol, 2004;15(1):4-19.
      Species: Human
      Sample Type: Exhaled Breath Condensate (EBC)
    17. A double-blind, placebo-controlled immunotherapy dose-response study with standardized cat extract.
      Authors: Ewbank PA, Murray J, Sanders K, Curran-Everett D, Dreskin S, Nelson HS
      J. Allergy Clin. Immunol., 2003;111(1):155-61.
      Species: Human
      Sample Type: Nasal Fluid
    18. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
      Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
      Scand. J. Rheumatol., 2002;31(4):205-10.
      Species: Human
      Sample Type: Serum
    19. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
      Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
      Infect. Immun., 2001;69(1):288-96.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
      Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
      Pediatr Allergy Immunol, 2000;11(1):20-8.
      Species: Human
      Sample Type: nasal lavage
    21. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
      Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
      Oral Microbiol Immunol, 2000;15(2):74-81.
      Species: Human
      Sample Type: Saliva
    Expand to show all
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    1. Why is plasma a validated sample type for the Human IL-4 Quantikine® ELISA (Catalog # D4050), but not in the high sensitivity version (Catalog # HS400)?

      • The recovery and linearity obtained with plasma samples assayed with the high sensitivity kit HS400 show too much variability and did not meet our specifications, unlike the D4050 which shows reliable recovery and linearity data with plasma samples.

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