Human IL-4 Quantikine HS ELISA Kit

Catalog # Availability Size / Price Qty
HS400
PHS400
SS400
Control Products Available
Human IL-4 High Sensitivity ELISA Cell Culture Supernate Standard Curve
3 Images
Product Details
Procedure
Citations (24)
FAQs
Supplemental Products
Reviews

Human IL-4 Quantikine HS ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
6.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL)
Sensitivity
0.22 pg/mL
Assay Range
0.3 - 16 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant human IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine HS Human IL-4 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.68 3.79 12.3 0.61 3.56 11.1
Standard Deviation 0.05 0.3 0.94 0.06 0.29 0.9
CV% 7.4 7.9 7.6 9.8 8.1 8.1

Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.75 5.56 10.7 0.59 3.32 10.7
Standard Deviation 0.05 0.28 0.29 0.06 0.19 0.79
CV% 2.9 5 2.7 10.2 5.7 7.4

Recovery

The recovery of IL-4 was determined by spiking to levels throughout the range of the assay in various matrices.

Sample Type Average % Recovery Range %
Cell Culture Media (n=9) 106 87-118
Serum (n=4) 102 95-108

Linearity

To assess linearity of the assay, the following samples, spiked with high concentrations of IL-4, were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-4 High Sensitivity ELISA Linearity

Data Examples

Human IL-4 High Sensitivity ELISA Cell Culture Supernate Standard Curve

Human IL-4 High Sensitivity ELISA Serum Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 50 µL Substrate Solution
  12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.

  13. 50 µL Amplifier Solution
  14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

  15. 50 µL Stop Solution
  16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.

Citations for Human IL-4 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Metabolites of L-ARG in Exhaled Breath Condensate and Serum Are Not Biomarkers of Bronchial Asthma in Children
    Authors: J Po?omska, B Soza?ska
    Journal of Clinical Medicine, 2022;11(1):.
    Species: Human
    Sample Types: Serum
  2. Accuracy of an experimental whole-blood test for detecting reactivation of echinococcal cysts
    Authors: L Petrone, F Tamarozzi, A Vola, MA Gomez Mora, A Ludovisi, S Najafi Far, M Mariconti, E Brunetti, D Goletti
    PloS Neglected Tropical Diseases, 2021;15(8):e0009648.
    Species: Human
    Sample Types: Plasma
  3. Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression
    Authors: K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz
    International Journal of Molecular Sciences, 2021;22(22):.
    Species: Human
    Sample Types: Plasma
  4. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men.
    Authors: Weng T, Huang S, Chuang Y, Wang J
    PLoS ONE, 2013;8(11):e80248.
    Species: Human
    Sample Types: Plasma
  5. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
    Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
    J. Immunol., 2011;186(9):5254-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Subnormal cytokine profile in the tear fluid of keratoconus patients.
    Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
    PLoS ONE, 2011;6(1):e16437.
    Species: Human
    Sample Types: Tears
  7. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
    Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
    Mol. Cell Proteomics, 2010;9(2):327-35.
    Species: Human
    Sample Types: Plasma
  8. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  9. Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
    Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
    Proteomics, 2008;8(11):2211-9.
    Species: Human
    Sample Types: Serum
  10. Dysregulated monocyte iron homeostasis and erythropoietin formation in patients with anemia of chronic disease.
    Authors: Theurl I, Mattle V, Seifert M, Mariani M, Marth C, Weiss G
    Blood, 2006;107(10):4142-8.
    Species: Human
    Sample Types: Serum
  11. Anti-inflammatory cytokines in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis: a preliminary report.
    Authors: Bozkurt FY, Yetkin Ay Z, Berker E, Tepe E, Akkus S
    Cytokine, 2006;35(3):180-5.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  12. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Types: Plasma
  13. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  14. Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
    Authors: Bartosik-Psujek H, Stelmasiak Z
    J Neural Transm, 2005;112(6):797-803.
    Species: Human
    Sample Types: Serum
  15. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Types: Serum
  16. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  17. Immunodetection of a hepatitis C virus (HCV) antigen and Thl/Th2 cytokines in cerebrospinal fluid of meningitis patients.
    Authors: Attallah AM, Ibrahim GG
    J Immunoassay Immunochem, 2004;25(4):313-20.
    Species: Human
    Sample Types: Serum
  18. Antifungal immune reactivity in nasal polyposis.
    Authors: Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L
    Infect. Immun., 2004;72(12):7275-81.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  19. Exhaled breath condensate in children: pearls and pitfalls.
    Authors: Rosias PP, Dompeling E, Hendriks HJ, Heijnens JW, Donckerwolcke RA, Jobsis Q
    Pediatr Allergy Immunol, 2004;15(1):4-19.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  20. A double-blind, placebo-controlled immunotherapy dose-response study with standardized cat extract.
    Authors: Ewbank PA, Murray J, Sanders K, Curran-Everett D, Dreskin S, Nelson HS
    J. Allergy Clin. Immunol., 2003;111(1):155-61.
    Species: Human
    Sample Types: Nasal Fluid
  21. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002;31(4):205-10.
    Species: Human
    Sample Types: Serum
  22. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
    Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
    Infect. Immun., 2001;69(1):288-96.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
    Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
    Pediatr Allergy Immunol, 2000;11(1):20-8.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  24. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
    Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
    Oral Microbiol Immunol, 2000;15(2):74-81.
    Species: Human
    Sample Types: Saliva

FAQs

  1. Why is plasma a validated sample type for the Human IL-4 Quantikine® ELISA (Catalog # D4050), but not in the high sensitivity version (Catalog # HS400)?

    • The recovery and linearity obtained with plasma samples assayed with the high sensitivity kit HS400 show too much variability and did not meet our specifications, unlike the D4050 which shows reliable recovery and linearity data with plasma samples.

View all ELISA FAQs

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