Human IL-5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF205

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Human IL-5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-5 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-5 is observed under non-reducing conditions.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-5
Ile20-Ser134
Accession # P05113
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑5 (Catalog # 205-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3.

Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Reviews for Human IL-5 Biotinylated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human IL-5 Biotinylated Antibody
By Alisha Freeman on 04/05/2018
Application: ELISA Sample Tested: 4T1.2 mouse mammary carcinoma cells Species: Mouse

Human IL-5 Biotinylated Antibody
By Alisha Freeman on 11/04/2017
Application: ELISA Sample Tested: Breast cancer cells Species: Human