Human IL-6 Luminex Performance Assay

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Citations (48)
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Human IL-6 Luminex Performance Assay Summary

Panel Luminex Performance Human Obesity Panel
Luminex Performance Human Cytokine Panel A
Assay Type Polystyrene bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Polystyrene antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

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Product Features

Polystyrene Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human IL-6 with up to analytes of your choice in one sample
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One filter-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Polystyrene beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Polystyrene Bead-based Multiplex Kits For The Luminex® Platform

Polystyrene Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaPolystyrene microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Product Datasheets

Citations for Human IL-6 Luminex Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

48 Citations: Showing 1 - 10
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  1. Plasma inflammatory cytokines and survival of pancreatic cancer patients
    Authors: A Babic, N Schnure, NP Neupane, MM Zaman, N Rifai, MW Welch, LK Brais, DA Rubinson, V Morales-Oy, C Yuan, S Zhang, EM Poole, BM Wolpin, MH Kulke, DA Barbie, K Wong, CS Fuchs, K Ng
    Clin Transl Gastroenterol, 2018;9(4):145.
    Species: Human
    Sample Types: Plasma
    Applications: Premixed Magnetic Luminex Assa
  2. C-reactive protein (CRP), interferon gamma-inducible protein 10 (IP-10), and lipopolysaccharide (LPS) are associated with risk of tuberculosis after initiation of antiretroviral therapy in resource-limited settings.
    Authors: Tenforde M, Gupte N, Dowdy D, Asmuth D, Balagopal A, Pollard R, Sugandhavesa P, Lama J, Pillay S, Cardoso S, Pawar J, Santos B, Riviere C, Mwelase N, Kanyama C, Kumwenda J, Hakim J, Kumarasamy N, Bollinger R, Semba R, Campbell T, Gupta A
    PLoS ONE, 2015;10(2):e0117424.
    Species: Human
    Sample Types: Plasma
  3. Polycystin deficiency induces dopamine-reversible alterations in flow-mediated dilatation and vascular nitric oxide release in humans.
    Authors: Lorthioir A, Joannides R, Remy-Jouet I, Freguin-Bouilland C, Iacob M, Roche C, Monteil C, Lucas D, Renet S, Audrezet M, Godin M, Richard V, Thuillez C, Guerrot D, Bellien J
    Kidney Int, 2015;87(2):465-72.
    Species: Human
    Sample Types: Plasma
  4. Differential cytokine profiles upon comparing selective versus classic glucocorticoid receptor modulation in human peripheral blood mononuclear cells and inferior turbinate tissue.
    Authors: Beck I, Van Crombruggen K, Holtappels G, Daubeuf F, Frossard N, Bachert C, De Bosscher K
    PLoS ONE, 2015;10(4):e0123068.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. The role of NOD2 in murine and human melioidosis.
    Authors: Myers N, Chantratita N, Berrington W, Chierakul W, Limmathurotsakul D, Wuthiekanun V, Robertson J, Liggitt H, Peacock S, Skerrett S, West T
    J Immunol, 2014;192(1):300-7.
    Species: Human
    Sample Types: Plasma
  6. Dietary factors associated with subclinical inflammation among girls.
    Authors: Del Mar Bibiloni M, Maffeis C, Llompart I, Pons A, Tur J
    Eur J Clin Nutr, 2013;67(12):1264-70.
    Species: Human
    Sample Types: Serum
  7. Survey of innate immune responses to Burkholderia pseudomallei in human blood identifies a central role for lipopolysaccharide.
    Authors: Chantratita N, Tandhavanant S, Myers N, Seal S, Arayawichanont A, Kliangsa-Ad A, Hittle L, Ernst R, Emond M, Wurfel M, Day N, Peacock S, West T
    PLoS ONE, 2013;8(11):e81617.
    Species: Human
    Sample Types: Plasma
  8. ROS-induced autophagy in cancer cells assists in evasion from determinants of immunogenic cell death.
    Authors: Garg, Abhishek, Dudek, Aleksand, Ferreira, Gabriela, Verfaillie, Tom, Vandenabeele, Peter, Krysko, Dmitri V, Mathieu, Chantal, Agostinis, Patrizia
    Autophagy, 2013;9(9):1292-307.
    Species: Human
    Sample Types: Whole Cells
  9. Sputum IgE and cytokines in asthma: relationship with sputum cellular profile.
    Authors: Manise M, Holtappels G, Van Crombruggen K, Schleich F, Bachert C, Louis R
    PLoS ONE, 2013;8(0):U111.
    Species: Human
    Sample Types: Sputum
  10. REDD1 protects osteoblast cells from gamma radiation-induced premature senescence.
    Authors: Li XH, Ha CT, Fu D, Xiao M
    PLoS ONE, 2012;7(5):e36604.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Inflammatory markers and metabolic syndrome among adolescents.
    Eur J Clin Nutr, 2012;66(10):1141-5.
    Species: Human
    Sample Types: Serum
  12. Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
    Authors: Lund TC, Stadem PS, Panoskaltsis-Mortari A, Raymond G, Miller WP, Tolar J, Orchard PJ
    PLoS ONE, 2012;7(2):e32218.
    Species: Human
    Sample Types: Serum
  13. Effects of methylprednisolone infusion on markers of inflammation, coagulation, and angiogenesis in early acute respiratory distress syndrome.
    Authors: Seam N, Meduri GU, Wang H, Nylen ES, Sun J, Schultz MJ, Tropea M, Suffredini AF
    Crit. Care Med., 2012;40(2):495-501.
    Species: Human
    Sample Types: Plasma
  14. Role of toll like receptors in irritable bowel syndrome: differential mucosal immune activation according to the disease subtype.
    Authors: Belmonte L, Beutheu Youmba S, Bertiaux-Vandaele N, Antonietti M, Lecleire S, Zalar A, Gourcerol G, Leroi A, Dechelotte P, Coeffier M, Ducrotte P
    PLoS ONE, 2012;7(8):e42777.
    Species: Human
    Sample Types: Tissue Homogenates
  15. The degree of leukocytosis and urine GATA-3 mRNA levels are risk factors for severe acute kidney injury in Puumala virus nephropathia epidemica.
    Authors: Libraty DH, Makela S, Vlk J, Hurme M, Vaheri A, Ennis FA, Mustonen J
    PLoS ONE, 2012;7(4):e35402.
    Species: Human
    Sample Types: Plasma
  16. Evaluation of the role of Candida albicans agglutinin-like sequence (Als) proteins in human oral epithelial cell interactions.
    Authors: Murciano C, Moyes DL, Runglall M, Tobouti P, Islam A, Hoyer LL, Naglik JR
    PLoS ONE, 2012;7(3):e33362.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Macrophage infiltration predicts a poor prognosis for human ewing sarcoma.
    Authors: Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y
    Am. J. Pathol., 2011;179(3):1157-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Anti-Inflammatory Cytokines Predominate in Acute Human Plasmodium knowlesi Infections.
    Authors: Cox-Singh J, Singh B, Daneshvar C, Planche T, Parker-Williams J, Krishna S
    PLoS ONE, 2011;6(6):e20541.
    Species: Human
    Sample Types: Serum
  19. The novel antisense Bcl-2 inhibitor SPC2996 causes rapid leukemic cell clearance and immune activation in chronic lymphocytic leukemia.
    Authors: Durig J, Duhrsen U, Klein-Hitpass L, Worm J, Hansen JB, Orum H, Wissenbach M
    Leukemia, 2011;25(4):638-47.
    Species: Human
    Sample Types: Serum
  20. Pro- and anti-inflammatory cytokines in latent autoimmune diabetes in adults, type 1 and type 2 diabetes patients: Action LADA 4.
    Authors: Pham MN, Hawa MI, Pfleger C, Roden M, Schernthaner G, Pozzilli P, Buzzetti R, Scherbaum W, Seissler J, Kolb H, Hunter S, Leslie RD, Schloot NC
    Diabetologia, 2011;54(7):1630-8.
    Species: Human
    Sample Types: Serum
  21. Candida albicans yeast and hyphae are discriminated by MAPK signaling in vaginal epithelial cells.
    Authors: Moyes DL, Murciano C, Runglall M, Islam A, Thavaraj S, Naglik JR
    PLoS ONE, 2011;6(11):e26580.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Lipid-heparin infusion suppresses the IL-10 response to trauma in subcutaneous adipose tissue in humans.
    Authors: Ikeoka DT, Pachler C, Mader JK, Bock G, Neves AL, Svehlikova E, Feichtner F, Koehler G, Wrighton CJ, Pieber TR, Ellmerer M
    Obesity (Silver Spring), 2011;19(4):715-21.
    Species: Human
    Sample Types: Complex Sample Type
  23. HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma.
    Authors: Buglio D, Khaskhely NM, Voo KS, Martinez-Valdez H, Liu YJ, Younes A
    Blood, 2011;117(10):2910-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
    Authors: Liang X, Moseman EA, Farrar MA
    Blood, 2010;115(24):5041-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Serum IL-6: a candidate biomarker for intracranial pressure elevation following isolated traumatic brain injury.
    Authors: Hergenroeder GW, Moore AN, McCoy JP
    J Neuroinflammation, 2010;7(0):19.
    Species: Human
    Sample Types: Serum
  26. Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component.
    Authors: O'Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, Bakke J, Mirsalis J, d'Andrea AL, Hume SD, Bratcher B, Saucedo CJ, McMahon JB, Pogue GP, Palmer KE
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(15):6099-104.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers.
    Authors: Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF
    Am. J. Respir. Crit. Care Med., 2009;179(12):1107-14.
    Species: Human
    Sample Types: BALF
  28. Endothelial dysfunction and specific inflammation in obesity hypoventilation syndrome.
    Authors: Borel JC, Roux-Lombard P, Tamisier R, Arnaud C, Monneret D, Arnol N, Baguet JP, Levy P, Pepin JL
    PLoS ONE, 2009;4(8):e6733.
    Species: Human
    Sample Types: Serum
  29. The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells.
    Authors: Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stockl J
    J. Immunol., 2009;183(7):4440-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Clinical efficacy and immune regulation with peanut oral immunotherapy.
    Authors: Jones SM, Pons L, Roberts JL, Scurlock AM, Perry TT, Kulis M, Shreffler WG, Steele P, Henry KA, Adair M, Francis JM, Durham S, Vickery BP, Zhong X, Burks AW
    J. Allergy Clin. Immunol., 2009;124(2):292-300, 300..
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Cigarette smoke facilitates allergen penetration across respiratory epithelium.
    Authors: Gangl K, Reininger R, Bernhard D, Campana R, Pree I, Reisinger J, Kneidinger M, Kundi M, Dolznig H, Thurnher D, Valent P, Chen KW, Vrtala S, Spitzauer S, Valenta R, Niederberger V
    Allergy, 2009;64(3):398-405.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Effect of systemic inflammation on inspiratory and limb muscle strength and bulk in cystic fibrosis.
    Authors: Dufresne V, Knoop C, Van Muylem A, Malfroot A, Lamotte M, Opdekamp C, Deboeck G, Cassart M, Stallenberg B, Casimir G, Duchateau J, Estenne M
    Am. J. Respir. Crit. Care Med., 2009;180(2):153-8.
    Species: Human
    Sample Types: Serum
  33. Critical and differential roles of NKp46- and NKp30-activating receptors expressed by uterine NK cells in early pregnancy.
    Authors: El Costa H, Casemayou A, Aguerre-Girr M, Rabot M, Berrebi A, Parant O, Clouet-Delannoy M, Lombardelli L, Jabrane-Ferrat N, Rukavina D, Bensussan A, Piccinni MP, Le Bouteiller P, Tabiasco J
    J. Immunol., 2008;181(0):3009-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
    Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
    J. Virol., 2008;82(7):3736-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Simvastatin attenuates release of neutrophilic and remodeling factors from primary bronchial epithelial cells derived from stable lung transplant recipients.
    Authors: Murphy DM, Forrest IA, Corris PA, Johnson GE, Small T, Jones D, Fisher AJ, Egan JJ, Cawston TE, Ward C, Lordan JL
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(3):L592-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. An evaluation of commercial fluorescent bead-based luminex cytokine assays.
    Authors: Djoba Siawaya JF, Roberts T, Babb C, Black G, Golakai HJ, Stanley K, Bapela NB, Hoal E, Parida S, van Helden P, Walzl G
    PLoS ONE, 2008;3(7):e2535.
    Species: Human
    Sample Types: Serum
  37. Defining cancer cachexia in head and neck squamous cell carcinoma.
    Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
    Clin. Cancer Res., 2007;13(22):6561-7.
    Species: Human
    Sample Types: Serum
  38. Circulating levels of cytokines during pregnancy: thrombopoietin is elevated in miscarriage.
    Authors: Whitcomb BW, Schisterman EF, Klebanoff MA, Baumgarten M, Luo X, Chegini N
    Fertil. Steril., 2007;89(6):1795-802.
    Species: Human
    Sample Types: Serum
  39. Haemophilus influenzae induces neutrophil necrosis: a role in chronic obstructive pulmonary disease?
    Authors: Naylor EJ, Bakstad D, Biffen M, Thong B, Calverley P, Scott S, Hart CA, Moots RJ, Edwards SW
    Am. J. Respir. Cell Mol. Biol., 2007;37(2):135-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Pronounced elevation of resistin correlates with severity of disease in severe sepsis and septic shock.
    Authors: Sunden-Cullberg J, Nystrom T, Lee ML, Mullins GE, Tokics L, Andersson J, Norrby-Teglund A, Treutiger CJ
    Crit. Care Med., 2007;35(6):1536-42.
    Species: Human
    Sample Types: Serum
  41. Peroxidated squalene induces the production of inflammatory mediators in HaCaT keratinocytes: a possible role in acne vulgaris.
    Authors: Ottaviani M, Alestas T, Flori E, Mastrofrancesco A, Zouboulis CC, Picardo M
    J. Invest. Dermatol., 2006;126(11):2430-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Sensitization of IL-2 signaling through TLR-7 enhances B lymphoma cell immunogenicity.
    Authors: Tomic J, White D, Shi Y, Mena J, Hammond C, He L, Miller RL, Spaner DE
    J. Immunol., 2006;176(6):3830-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers.
    Authors: Alexis NE, Lay JC, Zeman K, Bennett WE, Peden DB, Soukup JM, Devlin RB, Becker S
    J. Allergy Clin. Immunol., 2006;117(6):1396-403.
    Species: Human
    Sample Types: Saliva
  44. Coengagement of CD16 and CD94 receptors mediates secretion of chemokines and induces apoptotic death of naive natural killer cells.
    Authors: Jewett A, Cacalano NA, Head C, Teruel A
    Clin. Cancer Res., 2006;12(7):1994-2003.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Secretion of proinflammatory cytokines and chemokines during amphotericin B exposure is mediated by coactivation of toll-like receptors 1 and 2.
    Authors: Razonable RR, Henault M, Lee LN, Laethem C, Johnston PA, Watson HL, Paya CV
    Antimicrob. Agents Chemother., 2005;49(4):1617-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.
    Authors: Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, Blazar BR, Chen W
    J. Immunol., 2004;173(7):4433-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers.
    Authors: Khan SS, Smith MS, Reda D, Suffredini AF, McCoy JP
    Cytometry B Clin Cytom, 2004;61(1):35-9.
    Species: Human
    Sample Types: Serum


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