Human IL-6 Magnetic Luminex® Performance Assay
Build your own Luminex Assay with our Luminex Assay Customization Tool.
U.S. orders usually ship in 2-3 business days
Build Now
Human IL-6 Magnetic Luminex® Performance Assay Summary
| Panel | Human Cytokine Panel A Human Obesity Panel |
| Assay Type | Magnetic bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and Magnetic antibody-coated beads |
| Species | Human |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. |
Make your selections below to configure your assay
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human IL-6 with up to analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.
- User-mix antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. |
||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 25 | 25 | 25 |
| Mean (pg/mL) | 30.2 | 180 | 903 | 34 | 189 | 900 |
| Standard deviation | 1.43 | 7.78 | 41.9 | 3.00 | 13.0 | 53.0 |
| CV (%) | 4.7 | 4.3 | 4.6 | 7.9 | 6.7 | 5.9 |
Recovery
|
Samples containing and/or spiked with human IL-6 were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range (%) | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 96 | 91-100 | ||||
| Serum | 108 | 95-118 | ||||
| EDTA plasma | 102 | 95-116 | ||||
| Heparin plasma | 109 | 96-129 | ||||
Linearity
|
Samples containing and/or spiked with human IL-6 were serially diluted to evaluate assay linearity. |
|||||||||
| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 101 | 106 | 103 | 99 | ||||
| Range (%) | 87-117 | 104-109 | 82-124 | 86-110 | |||||
| 1:4 | Average % of Expected | 103 | 112 | 122 | 101 | ||||
| Range (%) | 86-127 | 103-117 | 116-129 | 85-114 | |||||
| 1:8 | Average % of Expected | 102 | 115 | 126 | 100 | ||||
| Range (%) | 82-130 | 97-126 | 121-131 | 87-110 | |||||
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Scientific Data
View Larger
Quantist software graph of signal intensity versus concentration for IL-6 in the Human Cytokine A Luminex Panel
Background: IL-6
Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.
IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor.
IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.
Citations for Human IL-6 Magnetic Luminex® Performance Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Insulin Resistance in Women Correlates with Chromatin Histone Lysine Acetylation, Inflammatory Signaling, and Accelerated Aging
Authors: Vidal, CM;Alva-Ornelas, JA;Chen, NZ;Senapati, P;Tomsic, J;Robles, VM;Resto, C;Sanchez, N;Sanchez, A;Hyslop, T;Emwas, N;Aljaber, D;Bachelder, N;Martinez, E;Ann, D;Jones, V;Winn, RA;Miele, L;Ochoa, AC;Dietze, EC;Natarajan, R;Schones, D;Seewaldt, VL;
Cancers
Species: Human
Sample Types: Serum
-
Coronary Artery Disease in Persons With Human Immunodeficiency Virus Without Detectable Viral Replication
Authors: Knudsen, AD;Fuchs, A;Benfield, T;Gerstoft, J;Køber, L;Trøseid, M;Kofoed, KF;Nielsen, SD;
Open forum infectious diseases
Species: Human
Sample Types: Plasma
-
25-hydroxycholesterol exacerbates vascular leak during acute lung injury
Authors: JH Madenspach, ED Morrell, JG McDonald, BM Thompson, Y Li, KG Birukov, AA Birukova, RD Stapleton, A Alejo, PW Karmaus, JM Meacham, P Rai, C Mikacenic, MM Wurfel, MB Fessler
JCI Insight, 2023-04-10;0(0):.
Species: Human
Sample Types: BALF
-
SARS-CoV-2 Infection Modulates ACE2 Function and Subsequent Inflammatory Responses in Swabs and Plasma of COVID-19 Patients
Authors: L Gutiérrez-, E Riveira-Mu, C Barrios, V Palau, M Nevot, S Pedreño-Ló, J Senserrich, M Massanella, B Clotet, C Cabrera, O Mitjà, M Crespo, J Pascual, M Riera, E Ballana
Viruses, 2021-08-28;13(9):.
Species: Human
Sample Types: Plasma
-
IRE1 Alpha/XBP1 Axis Sustains Primary Effusion Lymphoma Cell Survival by Promoting Cytokine Release and STAT3 Activation
Authors: R Gonnella, MS Gilardini, L Guttieri, MA Romeo, R Santarelli, M Cirone
Biomedicines, 2021-01-27;9(2):.
Species: Human
Sample Types: Cell Culture Supernates
-
Disharmonic Inflammatory Signatures in COVID-19: Augmented Neutrophils' but Impaired Monocytes' and Dendritic Cells' Responsiveness
Authors: Z Parackova, I Zentsova, M Bloomfield, P Vrabcova, J Smetanova, A Klocperk, G Mesežnikov, LF Casas Mend, T Vymazal, A Sediva
Cells, 2020-09-29;9(10):.
Species: Human
Sample Types: Cell Culture Supernates
-
Endo-1,3(4)-beta-Glucanase-Treatment of Oat beta-Glucan Enhances Fermentability by Infant Fecal Microbiota, Stimulates Dectin-1 Activation and Attenuates Inflammatory Responses in Immature Dendritic Cells
Authors: R Akkerman, MJ Logtenberg, R An, MA Van Den Be, BJ de Haan, MM Faas, E Zoetendal, P de Vos, HA Schols
Nutrients, 2020-06-03;12(6):.
Species: Human
Sample Types: Cell Culture Supernates
-
Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor
Authors: J Ho, X Yang, SA Nikou, N Kichik, A Donkin, NO Ponde, JP Richardson, RL Gratacap, LS Archambaul, CP Zwirner, C Murciano, R Henley-Smi, S Thavaraj, CJ Tynan, SL Gaffen, B Hube, RT Wheeler, DL Moyes, JR Naglik
Nat Commun, 2019-05-24;10(1):2297.
Species: Human
Sample Types: Cell Culture Supernates
-
The importance of inter-individual Kupffer cell variability in the governance of hepatic toxicity in a 3D primary human liver microtissue model
Authors: A Kermanizad, DM Brown, W Moritz, V Stone
Sci Rep, 2019-05-13;9(1):7295.
Species: Human
Sample Types: Cell Culture Supernates
-
A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis
Authors: D Devos, C Moreau, M Kyheng, G Garçon, AS Rolland, H Blasco, P Gelé, T Timothée L, C Veyrat-Dur, P Corcia, M Dutheil, P Bede, A Jeromin, P Oeckl, M Otto, V Meninger, V Danel-Brun, JC Devedjian, JA Duce, PF Pradat
Sci Rep, 2019-02-27;9(1):2918.
Species: Human
Sample Types: Plasma
-
A randomised study in young subjects of the effects of eating extra fruit or nuts on periodontal inflammation
BDJ Open, 2018-02-09;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Processing of Candida albicans Ece1p Is Critical for Candidalysin Maturation and Fungal Virulence
Authors: JP Richardson, S Mogavero, DL Moyes, M Blagojevic, T Krüger, AH Verma, BM Coleman, J De La Cruz, D Schulz, NO Ponde, G Carrano, O Kniemeyer, D Wilson, O Bader, SI Enoiu, J Ho, N Kichik, SL Gaffen, B Hube, JR Naglik
MBio, 2018-01-23;9(1):.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
No product specific FAQs exist for this product, however you may
View all Luminex Assay FAQsReviews for Human IL-6 Magnetic Luminex® Performance Assay
Average Rating: 5 (Based on 3 Reviews)
Have you used Human IL-6 Magnetic Luminex® Performance Assay?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
IL-6 performed as expected with Low % CVs
