• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    18-24 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    0.1 pg/mL
  • Assay Range
    0.2 - 10 pg/mL (Cell Culture Supernates), 0.3 - 16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-7
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.51.496.860.782.138.81
Standard Deviation0.040.140.610.080.160.64
CV%89.48.910.37.57.3

Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.465.7911.180.516.3812.95
Standard Deviation0.030.190.440.060.51.3
CV%6.53.33.911.87.810

Recovery

The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 103 89-113
Citrate Plasma (n=5) 100 92-110
EDTA Plasma (n=5) 112 83-132
Serum (n=5) 100 91-113
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-7 Quantikine HS ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
    • Long Name
      Interleukin 7
    • Entrez Gene IDs
      3574 (Human); 16196 (Mouse); 25647 (Rat);
    • Alternate Names
      IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 14-20 hours.
    7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 6 times.

    11. 50 µL Substrate Solution
    12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes. Do not wash the plate.

    13. 50 µL Amplifier Solution
    14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    33 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis
      Authors: C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen
      PLoS Pathog., 2017;13(6):e1006425.
      Species: Human
      Sample Type: Plasma
    2. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
      Authors: NP Kumar, VV Banurekha, D Nair, S Babu
      PLoS ONE, 2017;12(4):e0176495.
      Species: Human
      Sample Type: Plasma
    3. Establishment of IL-7 Expression Reporter Human Cell Lines, and Their Feasibility for High-Throughput Screening of IL-7-Upregulating Chemicals
      PLoS ONE, 2016;11(9):e0161899.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning.
      Authors: Hannon M, Beguin Y, Ehx G, Servais S, Seidel L, Graux C, Maertens J, Kerre T, Daulne C, De Bock M, Fillet M, Ory A, Willems E, Gothot A, Humblet-Baron S, Baron F
      Clin Cancer Res, 2015;21(14):3131-9.
      Species: Human
      Sample Type: Plasma
    5. Long-term control of simian immunodeficiency virus (SIV) in cynomolgus macaques not associated with efficient SIV-specific CD8+ T-cell responses.
      Authors: Bruel T, Hamimi C, Dereuddre-Bosquet N, Cosma A, Shin S, Corneau A, Versmisse P, Karlsson I, Malleret B, Targat B, Barre-Sinoussi F, Le Grand R, Pancino G, Saez-Cirion A, Vaslin B
      J Virol, 2015;89(7):3542-56.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Plasma
    6. Recycled IL-7 Can Be Delivered to Neighboring T Cells.
      Authors: Bazdar D, Kalinowska M, Panigrahi S, Sieg S
      J Immunol, 2015;194(10):4698-704.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques.
      Authors: Okoye A, Rohankhedkar M, Konfe A, Abana C, Reyes M, Clock J, Duell D, Sylwester A, Sammader P, Legasse A, Park B, Axthelm M, Nikolich-Zugich J, Picker L
      J Immunol, 2015;195(9):4292-305.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Plasma
    8. T cell reconstitution in allogeneic haematopoietic stem cell transplantation: prognostic significance of plasma interleukin-7.
      Authors: Kielsen K, Jordan K, Uhlving H, Pontoppidan P, Shamim Z, Ifversen M, Heilmann C, Nielsen C, Sengelov H, Ryder L, Muller K
      Scand J Immunol, 2015;81(1):72-80.
      Species: Human
      Sample Type: Plasma
    9. IFN-alpha exerts opposing effects on activation-induced and IL-7-induced proliferation of T cells that may impair homeostatic maintenance of CD4+ T cell numbers in treated HIV infection.
      Authors: Cha L, de Jong E, French M, Fernandez S
      J Immunol, 2014;193(5):2178-86.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis.
      Authors: Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M
      PLoS Pathog, 2014;10(3):e1003976.
      Species: Human
      Sample Type: Serum
    11. The systemic cytokine environment is permanently altered in multiple myeloma.
      Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
      PLoS ONE, 2013;8(3):e58504.
    12. Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
      Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
      PLoS ONE, 2013;8(12):e81983.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Up-regulation of intestinal epithelial cell derived IL-7 expression by keratinocyte growth factor through STAT1/IRF-1, IRF-2 pathway.
      Authors: Cai, Yu-Jiao, Wang, Wen-Shen, Yang, Yang, Sun, Li-Hua, Teitelbaum, Daniel H, Yang, Hua
      PLoS ONE, 2013;8(3):e58647.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
      Genes Immun, 2012;13(7):587-92.
      Species: Human
      Sample Type: Plasma
    15. Treatment with IL-7 prevents the decline of circulating CD4+ T cells during the acute phase of SIV infection in rhesus macaques.
      Authors: Vassena L, Miao H, Cimbro R, Malnati MS, Cassina G, Proschan MA, Hirsch VM, Lafont BA, Morre M, Fauci AS, Lusso P
      PLoS Pathog., 2012;8(4):e1002636.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Plasma
    16. Altered T cell memory and effector cell development in chronic lymphatic filarial infection that is independent of persistent parasite antigen.
      Authors: Steel C, Nutman TB
      PLoS ONE, 2011;6(4):e19197.
      Species: Human
      Sample Type: Serum
    17. Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD.
      Authors: Thiant S, Labalette M, Trauet J, Coiteux V, de Berranger E, Dessaint JP, Yakoub-Agha I
      Bone Marrow Transplant., 2010;46(10):1374-81.
      Species: Human
      Sample Type: Plasma
    18. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
      Authors: Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A
      Clin. Exp. Immunol., 2010;162(3):447-59.
      Species: Human
      Sample Type: Plasma
    19. Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse.
      Authors: Thiant S, Yakoub-Agha I, Magro L, Trauet J, Coiteux V, Jouet JP, Dessaint JP, Labalette M
      Bone Marrow Transplant., 2010;45(10):1546-52.
      Species: Human
      Sample Type: Plasma
    20. Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection.
      Authors: Sugalski JM, Rodriguez B, Moir S
      J. Immunol., 2010;185(5):3019-27.
      Species: Human
      Sample Type: Serum
    21. Dual mechanism of impairment of interleukin-7 (IL-7) responses in human immunodeficiency virus infection: decreased IL-7 binding and abnormal activation of the JAK/STAT5 pathway.
      Authors: Juffroy O, Bugault F, Lambotte O, Landires I, Viard JP, Niel L, Fontanet A, Delfraissy JF, Theze J, Chakrabarti LA
      J. Virol., 2010;84(1):96-108.
      Species: Human
      Sample Type: Plasma
    22. Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients.
      Authors: Louis CU, Straathof K, Bollard CM, Gerken C, Huls MH, Gresik MV, Wu MF, Weiss HL, Gee AP, Brenner MK, Rooney CM, Heslop HE, Gottschalk S
      Blood, 2009;113(11):2442-50.
      Species: Human
      Sample Type: Plasma
    23. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
      Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
      Int. J. Cancer, 2009;125(5):1092-101.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
      Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
      J. Immunol., 2009;182(12):7389-97.
      Species: Human
      Sample Type: Plasma
    25. Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
      Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
      Arthritis Rheum., 2008;58(11):3430-5.
      Species: Human
      Sample Type: Synovial Fluid
    26. Longitudinal analysis of immune function in the first 3 years of life in thymectomized neonates during cardiac surgery.
      Authors: Mancebo E, Clemente J, Sanchez J, Ruiz-Contreras J, De Pablos P, Cortezon S, Romo E, Paz-Artal E, Allende LM
      Clin. Exp. Immunol., 2008;154(3):375-83.
      Species: Human
      Sample Type: Plasma
    27. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
      Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
      J. Pathol., 2007;212(1):47-55.
      Species: Human
      Sample Type: Serum
    28. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
      Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
      J. Immunol., 2007;178(8):5340-50.
      Species: Human
      Sample Type: Serum
    29. Sleep enhances serum interleukin-7 concentrations in humans.
      Authors: Benedict C, Dimitrov S, Marshall L, Born J
      Brain Behav. Immun., 2007;21(8):1058-62.
      Species: Human
      Sample Type: Serum
    30. IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation.
      Authors: Koesters SA, Alimonti JB, Wachihi C, Matu L, Anzala O, Kimani J, Embree JE, Plummer FA, Fowke KR
      Eur. J. Immunol., 2006;36(2):336-44.
      Species: Human
      Sample Type: Plasma
    31. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
      Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
      J. Immunol., 2005;175(11):7325-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Elevated interleukin-7 levels not sufficient to maintain T-cell homeostasis during simian immunodeficiency virus-induced disease progression.
      Authors: Muthukumar A, Wozniakowski A, Gauduin MC, Paiardini M, McClure HM, Johnson RP, Silvestri G, Sodora DL
      Blood, 2004;103(3):973-9.
      Species: Primate - Cebus capucinus (White-headed Capuchin Monkey)
      Sample Type: Plasma
    33. Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys.
      Authors: Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP
      Blood, 2003;101(10):4209-18.
      Species: Primate - Papio cynocephalus (Yellow Baboon)
      Sample Type: Serum
    Expand to show all 33 Citations
    Average Rating: 5 (Based on 1 review)

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