Human IL-7 R alpha /CD127 PE-conjugated Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.
  • Source
    Monoclonal Mouse IgG1 Clone # 40131
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha
    Extracellular domain
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human
IL‑7 R alpha /CD127 PE‑conjugated Monoclonal Antibody
(Catalog # FAB306P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-7 R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

  • References:
    1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
    2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
    3. Goodwin, R.G. et al. (1990) Cell 60:941.
    4. Noguchi, M. et al. (1993) Science 262:1877.
    5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
    6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
    7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
    8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
    9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
    10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
    11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
    13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
    14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
    15. Jahn, T. et al. (2007) Blood 110:1840.
    16. Lai, L. et al. (2006) Blood 107:1776.
  • Long Name:
    Interleukin 7 Receptor alpha
  • Entrez Gene IDs:
    3575 (Human); 16197 (Mouse); 294797 (Rat)
  • Alternate Names:
    CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
    Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
    J. Immunol., 2007;178(8):5340-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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