Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 40131
View all IL-7R alpha/CD127 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha
Extracellular domain

Specificity

Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-7R alpha/CD127 PE-conjugated Antibody

Detection of IL-7 Ra/CD127 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB306P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-7R alpha/CD127 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Reviewed Applications

Read 2 reviews rated 4 using FAB306P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Gene Symbol

IL7R

Additional IL-7R alpha/CD127 Products

Product Documents for Human IL-7R alpha/CD127 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-7R alpha/CD127 PE-conjugated Antibody

For research use only

Citations for Human IL-7R alpha/CD127 PE-conjugated Antibody

Customer Reviews for Human IL-7R alpha/CD127 PE-conjugated Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

Have you used Human IL-7R alpha/CD127 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-7 R alpha /CD127 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: CD8 T lymphocytes
    Species: Human
    Verified Customer | Posted 02/08/2019
    CD127 staining of activated primary human CD8+ T-cells.
    Primary human CD8+ T-cells were isolated from PBMCs and activated with anti-CD3/anti-CD28 coated beads in culture.
    Human IL-7R alpha/CD127 PE-conjugated Antibody FAB306P
  • Human IL-7 R alpha /CD127 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 07/07/2017

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies