Human IL-7R alpha/CD127 Antibody

Name: Human IL-7R alpha/CD127 Antibody
Brand: R&D Systems
SKU: MAB306-500
Rating: 5 (1 reviews)

Catalog #: MAB306
14 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB306-SP
MAB306-100
MAB306-500

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry.

1 Image

View All Products for IL-7R alpha/CD127

Product Details

Citations (14)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-7R alpha/CD127 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human
(rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.

Source

Monoclonal Mouse IgG₁ Clone # 40131

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (Catalog # 306-IR)

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-/CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
Goodwin, R.G. et al. (1990) Cell 60:941.
Noguchi, M. et al. (1993) Science 262:1877.
Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
Jahn, T. et al. (2007) Blood 110:1840.
Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Alternate Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-7R alpha/CD127 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes
Authors: P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth
European Journal of Immunology, 2021-11-01;0(0):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture
Synergism between IL7R and CXCR4 drives BCR-ABL induced transformation in Philadelphia chromosome-positive acute lymphoblastic leukemia
Authors: H Abdelrasou, A Vadakumche, M Werner, L Lenk, A Khadour, M Young, O El Ayoubi, F Vogiatzi, M Krämer, V Schmid, Z Chen, Y Yousafzai, G Cario, M Schrappe, M Müschen, C Halsey, MA Mulaw, DM Schewe, E Hobeika, A Alsadeq, H Jumaa
Nat Commun, 2020-06-24;11(1):3194.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
IL-7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection
Authors: SC Côté, SCB Schinkel, TK Berthoud, PO Barros, M Sanchez-Vi, AM Davidson, AM Crawley, JB Angel
Eur. J. Immunol., 2020-05-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele
Authors: H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax
Nat Commun, 2019-10-08;10(1):4575.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Macrophages are the primary effector cells in IL-7-induced arthritis
Authors: SJ Kim, HJ Chang, MV Volin, S Umar, K Van Raemdo, A Chevalier, K Palasiewic, JW Christman, S Volkov, S Arami, M Maz, A Mehta, RK Zomorrodi, DA Fox, N Sweiss, S Shahrara
Cell. Mol. Immunol., 2019-06-13;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
CD56(bright) NK IL-7R? expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections
Authors: CJ Judge, L Kostadinov, KE Sherman, AA Butt, Y Falck-Ytte, NT Funderburg, AL Landay, MM Lederman, SF Sieg, JK Sandberg, DD Anthony
J. Leukoc. Biol., 2017-04-11;0(0):.
Species: Human
Sample Types: Serum
Applications: ELISA Development
IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study
PLoS Med, 2016-12-20;13(12):e1002200.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation.
Authors: Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont
Scientific Reports, 2016-02-26;0(0):2045-2322.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The clinical correlation of regulatory T cells and cyclic adenosine monophosphate in enterovirus 71 infection.
Authors: Wang, Shih-Min, Chen, I-Chun, Liao, Yu-Ting, Liu, Ching-Ch
PLoS ONE, 2014-07-10;9(7):e102025.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
Genes Immun, 2012-08-23;13(7):587-92.
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
J. Biol. Chem., 2010-02-18;285(20):14898-908.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot

Functionally significant differences in expression of disease-associated IL-7 receptor alpha haplotypes in CD4 T cells and dendritic cells.
Authors: Hoe E, McKay FC, Schibeci SD, Gandhi K, Heard RN, Stewart GJ, Booth DR
J. Immunol., 2010-01-22;184(5):2512-7.
Species: Human
Sample Types: Serum
Applications: ELISA Development

Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009-06-15;182(12):7389-97.
Species: Human
Sample Types: Plasma
Applications: ELISA Development, Immunoprecipitation

Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma.
Authors: Srivastava BI, Srivastava MD
Leuk. Res., 2005-07-01;29(7):771-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC