Human IL-9 APC-conjugated Antibody Summary
Accession # P15248
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑9 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Preparation and Storage
Interleukin-9 (IL-9) is a 28-30 kDa monomeric glycosylated polypeptide that belongs to the IL-7/IL-9 family of proteins. Although multiple cell types are reported to express IL-9, including Th17 cells, eosinophils, basophils, NK T cells and mast cells, the two cell types possibly most central to the process are Th9 and ILC-2 (or innate lymphoid cell type 2) cells. IL-9 supports the growth of multiple hematopoietic cell types including germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. It also promotes IgE production, the activation and chemoattraction of mast cells, mucus production by epithelium, Th17 cell proliferation and TGF-beta production by dendritic cells. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.
Citation for Human IL-9 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The Immunogenicity of Branded and Biosimilar Infliximab in Rheumatoid Arthritis According to Th9-Related Responses
Authors: R Talotta, A Berzi, A Doria, A Batticciot, MC Ditto, F Atzeni, P Sarzi-Putt, D Trabattoni
Int J Mol Sci, 2017;18(10):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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