Human IL-9 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC209A
Detection of IL‑9 in Human PBMCs by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human IL-9 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat IL-9 or recombinant mouse IL-9 is observed.
Source
Monoclonal Mouse IgG2B Clone # 623153
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑9 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-9

Interleukin-9 (IL-9) is a 28-30 kDa monomeric glycosylated polypeptide that belongs to the IL-7/IL-9 family of proteins. Although multiple cell types are reported to express IL-9, including Th17 cells, eosinophils, basophils, NK T cells and mast cells, the two cell types possibly most central to the process are Th9 and ILC-2 (or innate lymphoid cell type 2) cells. IL-9 supports the growth of multiple hematopoietic cell types including germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. It also promotes IgE production, the activation and chemoattraction of mast cells, mucus production by epithelium, Th17 cell proliferation and TGF-beta production by dendritic cells. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Product Datasheets

Citation for Human IL-9 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The Immunogenicity of Branded and Biosimilar Infliximab in Rheumatoid Arthritis According to Th9-Related Responses
    Authors: R Talotta, A Berzi, A Doria, A Batticciot, MC Ditto, F Atzeni, P Sarzi-Putt, D Trabattoni
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human IL-9 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human IL-9 APC-conjugated Antibody and earn rewards!

Have you used Human IL-9 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review