Detects human Integrin beta 1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin beta 1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, rmIntegrin beta 6, and rmIntegrin beta 7 is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A Gln21-Asp728 Accession # P05556
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human Integrin beta 1/CD29
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta 1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least
ten different integrin alpha subunits to form various VLA complexes. The beta 1
subunit has a broad tissue distribution except erythrocytes.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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