Detects human Integrin beta 1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin beta 1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, rmIntegrin beta 6, and rmIntegrin beta 7 is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A Gln21-Asp728 Accession # P05556
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human Integrin beta 1/CD29
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta 1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least
ten different integrin alpha subunits to form various VLA complexes. The beta 1
subunit has a broad tissue distribution except erythrocytes.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.