Human/Canine Integrin beta 1/CD29 Antibody

Name: Human/Canine Integrin beta 1/CD29 Antibody
Brand: R&D Systems
SKU: AF1778
Price: 519 USD
Availability: InStock

R&D Systems | Catalog # AF1778

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Citations (11)

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Canine

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Western Blot, Neutralization, Immunocytochemistry, Functional Assay, Migration Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556

Specificity

Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Canine Integrin beta 1/CD29 Antibody

Detection of Human Integrin beta 1/CD29 by Western Blot.

Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Integrin beta 1/CD29 by Simple Western^TM.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta 1/CD29 at approximately 198 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Detection of Integrin beta 1/CD29 in Canine PBMCs by Flow Cytometry.

Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells by Flow Cytometry.

Canine mesenchymal stem cells were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells.

Integrin beta 1/CD29 was detected in immersion fixed canine mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Integrin beta 1/CD29 in Human Mesenchymal Stem Cells.

Integrin beta 1/CD29 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Human Integrin beta 1/CD29 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Integrin beta 1/CD29 at approximately 157-176 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Integrin beta 1/CD29 in A549 Human Cell Line.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Shows Human Integrin beta 1/CD29 Specificity by Using Knockout Cell Line.

Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin beta 1/CD29 knockout A549 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 105 kDa and 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Applications for Human/Canine Integrin beta 1/CD29 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), canine peripheral blood mononuclear cells (PBMCs), and canine mesenchymal stem cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed canine mesenchymal stem cells, human mesenchymal stem cells, and A549 human lung carcinoma cell line

Knockout Validated

Integrin beta 1/CD29 is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.

Simple Western

10-20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line

Western Blot

1 µg/mL
Sample: MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes. Over aa 21-720, human Integrin beta 1 shares 95% aa identity with canine Integrin beta 1.