Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 413613
View all IRAK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3

Specificity

Detects human IRAK4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human IRAK4 Antibody

Detection of IRAK4 antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of IRAK4 in K562 Human Cell Line by Flow Cytometry.

K562 human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IRAK4 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of IRAK4 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
IRAK4 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

IRAK4 in U937 Human Cell Line.

IRAK4 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human IRAK4 Monoclonal Antibody (Catalog # MAB39191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human IRAK4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: K562 or Jurkat human cell line

Reviewed Applications

Read 1 review rated 5 using MAB39191 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRAK4

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Entrez Gene IDs

51135 (Human); 266632 (Mouse); 300177 (Rat)

Gene Symbol

IRAK4

UniProt

Q9NWZ3

Additional IRAK4 Products

Product Documents for Human IRAK4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRAK4 Antibody

For research use only

Customer Reviews for Human IRAK4 Antibody (1)

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  • Human IRAK4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 11/24/2021
    Human IRAK4 Antibody MAB39191

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Protocols

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