Human IRAK4 Antibody

Catalog # Availability Size / Price Qty
MAB39191-100
MAB39191-SP
Human IRAK4 Antibody in Intracellular Staining by Flow Cytometry
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Human IRAK4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRAK4 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 413613
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of IRAK4 in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of IRAK4 in K562 Human Cell Line by Flow Cytometry. K562 human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of IRAK4 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of IRAK4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry IRAK4 in U937 Human Cell Line. View Larger

IRAK4 in U937 Human Cell Line. IRAK4 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human IRAK4 Monoclonal Antibody (Catalog # MAB39191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRAK4

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Long Name
Interleukin-1 Receptor-Associated Kinase 4
Entrez Gene IDs
51135 (Human); 266632 (Mouse); 300177 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; interleukin-1 receptor-associated kinase 4; IPD1; IRAK4; IRAK-4; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG1 Isotype Control

Reconstitution Buffers

Secondary Antibodies

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