Human ITM2B Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ITM2B in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ITM2A and rhITM2C is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ITM2B
    Ala60-Ser266
    Accession # Q9Y287
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
ITM2B in Human Brain. ITM2B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human ITM2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7015) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ITM2B

ITM2B (Integral membrane protein 2B; also protein E25B and BRI/BRI2) is a 50 kDa member of the ITM2 family of proteins. It is widely expressed, and is involved in APP processing. ITM2B is expressed in the Golgi and on the cell surface, and forms a noncovalent complex with A beta PP. Although both molecules are susceptible to secretase processing, ITM2B appears to block A beta PP cleavage by alpha - and gamma -secretase. Human ITM2B is a 266 amino acid (aa) type II transmembrane protein. The ECD (aa 76-266) contains a propeptide sequence (aa 244-266), one BRICHOS domain (aa 137-231), and a Cys at position 89 that participates in homodimerization. Multiple enzymes can cleave ITM2B. Cleavage of the propeptide by furin generates a 3-4 kDa soluble fragment and a 41-45 kDa transmembrane molecule. Further cleavage of the 44 kDa form in the juxtamembrane and intramembrane regions generates a soluble 24 kDa BRICHOS-containing protein, a 22 kDa N-terminal transmembrane peptide, and a soluble 10 kDa cytoplasmic fragment. There are multiple potential splice variants. Two add different 11 aa sequences to the propeptide, resulting in two different 4-5 kDa, 34 aa fragments being released by furin activity. There is also a deletion of aa 83-188, and a substitution of 21 aa for aa 116-266. Over aa 60-266, human ITM2B shares 96% aa identity with mouse ITM2B.

  • Long Name:
    Integral Membrane Protein 2B
  • Entrez Gene IDs:
    9445 (Human); 16432 (Mouse); 290364 (Rat)
  • Alternate Names:
    ABRI; ABri/ADan amyloid peptide; BRI; BRI2; BRICD2B; BRICHOS domain containing 2B; BRIFBD; E25B; E3-16; FBD; integral membrane protein 2B; ITM2B; Protein E25B; Transmembrane protein BRI
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