Human LAMP2/CD107b Antibody
Human LAMP2/CD107b Antibody Summary
Accession # P13473
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAMP2/CD107b Monoclonal Antibody (Catalog # MAB6228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LAMP2/CD107b at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LAMP2/CD107b in HEK293 Human Cell Line. LAMP2/CD107b was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAMP2/CD107b Monoclonal Antibody (Catalog # MAB6228) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of LAMP2/CD107b in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LAMP2/CD107b Monoclonal Antibody (Catalog # MAB6228, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).
- Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
- Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
- Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
- Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
- Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
- Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
- Tanaka, Y. et al. (1990) Nature 406:902.
- Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
- Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
- Grutzkau, A. et al. (2004) Cytometry 61:62.
- Kannan, K. et al. (1996) Cell. Immunol. 171:10.
- Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
- Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
- Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
- Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human LAMP2/CD107b Antibody
There are currently no reviews for this product. Be the first to review Human LAMP2/CD107b Antibody and earn rewards!
Have you used Human LAMP2/CD107b Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image