Human Leukotriene B4 R1 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB099V-100UG
Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.
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Human Leukotriene B4 R1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Leukotriene B4 R1 (Pettersson, A. et al. (2000) Biochem. Biophys. Res. Commun. 279:520).
Source
Monoclonal Mouse IgG1 Clone # 203/14F11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

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Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Leukotriene B4 R1 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB099V) or (B) Mouse IgG1 Alexa Fluor® 405 Isotype Control (Catalog # IC002V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9).

References
  1. Samuelsson, B. et al. (1987) Science 237:1171.
  2. Brom, J. and W. Konig (1989) Immunology 68:479.
  3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
  4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
  5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
  6. Yokomizo, T. et al. (1997) Nature 387:620.
  7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
  8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
  9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
Long Name
Leukotriene B4 Receptor 1
Entrez Gene IDs
1241 (Human)
Alternate Names
BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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