Human Leukotriene B4 R1 Antibody (203/14F11)

R&D Systems | Catalog # MAB099

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Assay Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 203/14F11
View all Leukotriene B4 R1 Primary Antibodies »

Product Specifications

Immunogen

HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1

Specificity

Recognizes human Leukotriene B4 R1 (Pettersson, A. et al. (2000) Biochem. Biophys. Res. Commun.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Leukotriene B4 R1 Antibody (203/14F11)

Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Monoclonal Antibody (Catalog # MAB099) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Leukotriene B4 R1 Antibody (203/14F11)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood monocytes

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9).

References

  1. Samuelsson, B. et al. (1987) Science 237:1171.
  2. Brom, J. and W. Konig (1989) Immunology 68:479.
  3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
  4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
  5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
  6. Yokomizo, T. et al. (1997) Nature 387:620.
  7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
  8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
  9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.

Long Name

Leukotriene B4 Receptor 1

Alternate Names

BLT1, Leukotriene B4R1, LTB4R, LTB4R1

Entrez Gene IDs

1241 (Human)

Gene Symbol

LTB4R

Additional Leukotriene B4 R1 Products

Product Documents for Human Leukotriene B4 R1 Antibody (203/14F11)

Certificate of Analysis

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Product Specific Notices for Human Leukotriene B4 R1 Antibody (203/14F11)

For research use only

Citations for Human Leukotriene B4 R1 Antibody (203/14F11)

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